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Trasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells

Nature Biotechnology volume 16, pages 642–646 (1998)Cite this article

Abstract

We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endo-derm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.

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Authors and Affiliations

  1. Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, 01003

    Jose B. Cibelli, Joseph Jerry & James M. Robl

  2. Advanced Cell Technology, Inc., Worcester, MA, 01605

    Jose B. Cibelli, Stevem L. Stice, Paul J. Golueke, Jeff J. Kane, Cathy Blackwell, F. Abel Ponce de León & James M. Robl

  3. Animal Sciences, College of Agricultural, Food and Environmental Sciences, University of Minnesota, St Paul, MN, 55108

    F. Abel Ponce de León

Authors
  1. Jose B. Cibelli
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  2. Stevem L. Stice
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  3. Paul J. Golueke
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  4. Jeff J. Kane
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  5. Joseph Jerry
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  6. Cathy Blackwell
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  7. F. Abel Ponce de León
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  8. James M. Robl
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Correspondence to James M. Robl.

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Cibelli, J., Stice, S., Golueke, P. et al. Trasgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. Nat Biotechnol 16, 642–646 (1998). https://doi.org/10.1038/nbt0798-642

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