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Fiber-optic Sensor for Continuous Monitoring of Fermentation pH

Bio/Technology volume 11, pages 726–729 (1993)Cite this article

Abstract

We have developed a fiber-optic chemical sensor for on-line monitoring of fermentation pH. The sensor is based on a covalently bound fluorescent dye immobilized within a water-permeable polymer layer on an optical fiber. Measurements were performed on a portable fluorimeter and employed a ratiometric approach to account for system instabilities. We show that the use of this fiber-optic sensor provides fast, accurate and reliable measurements during E. coli fermentation in a complex medium.

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Author notes

  1. David R. Walt: Corresponding author.

Authors and Affiliations

  1. Department of Chemistry, Max Tishler Laboratory for Organic Chemistry, Tufts University, Medford, MA, 02155

    Venetka I. Agayn & David R. Walt

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  1. Venetka I. Agayn
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  2. David R. Walt
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Agayn, V., Walt, D. Fiber-optic Sensor for Continuous Monitoring of Fermentation pH. Nat Biotechnol 11, 726–729 (1993). https://doi.org/10.1038/nbt0693-726

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