Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Targeted mutagenesis in zebrafish

Nature Biotechnology volume 26, pages 650–651 (2008)Cite this article

Directed gene knockout in a vertebrate has been achieved using zinc-finger nucleases.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Targeted mutagenesis of zebrafish genes with zinc-finger nucleases (ZFNs).

Katie Ris-Vicari

References

  1. Amsterdam, A. & Hopkins, N. Trends Genet. 22, 473–478 (2006).

    Article  CAS  PubMed  Google Scholar 

  2. Meng, X. et al. Nat. Biotechnol. 26, 695–701 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Doyon, Y. et al. Nat. Biotechnol. 26, 702–708 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Porteus, M.H. & Baltimore, D. Science 300, 763 (2003).

    Article  PubMed  Google Scholar 

  5. Bibikova, M., Beumer, K., Trautman, J.K. & Carroll, D. Science 300, 764 (2003).

    Article  CAS  PubMed  Google Scholar 

  6. Wu, J., Kandavelou, K. & Chandrasegaran, S. Cell. Mol. Life Sci. 64, 2933–2944 (2007).

    Article  CAS  Google Scholar 

  7. Santiago, Y. et al. Proc. Natl. Acad. Sci. USA 105, 5809–5814 (2008).

    Article  PubMed  PubMed Central  Google Scholar 

  8. Bibikova, M., Golic, M., Golic, K.G. & Carroll, D. Genetics 161, 1169–1175 (2002).

    CAS  PubMed  PubMed Central  Google Scholar 

  9. Urnov, F.D. et al. Nature 435, 646–651 (2005).

    Article  CAS  PubMed  Google Scholar 

  10. Mandell, J.G. & Barbas, C.F. Nucleic Acids Res. 34, W516–523 (2006).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Carroll, D., Morton, J.J., Beumer, K.J. & Segal, D.J. Nat. Protocols 1, 1329–1341 (2006).

    Article  CAS  PubMed  Google Scholar 

  12. Ramirez, C.L. et al. Nat. Methods 5, 374–375 (2008).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Ian G. Woods and Alexander F. Schier are in the Department of Molecular and Cellular Biology, Broad Institute, Center for Brain Science, Harvard Stem Cell Institute, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA. schier@fas.harvard.edu or ianwoods@mcb.harvard.edu,

    Ian G Woods & Alexander F Schier

Authors
  1. Ian G Woods
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexander F Schier
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Woods, I., Schier, A. Targeted mutagenesis in zebrafish. Nat Biotechnol 26, 650–651 (2008). https://doi.org/10.1038/nbt0608-650

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0608-650

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing