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Expression of Human Anti-Hemophilic Factor IX in the Milk of Transgenic Sheep

Bio/Technology volume 7, pages 487–492 (1989)Cite this article

Abstract

Transgenic livestock may prove useful for the large scale production of valuable proteins. By targeting expression to the mammary gland these proteins could be harvested from milk. To this end, we have designed a hybrid gene to direct the synthesis of human anti-hemophilic factor IX to the mammary gland, and introduced it into sheep. Two transgenic ewes, each carrying about 10 copies of the foreign gene, have been analysed for expression. Both animals express human factor IX RNA in the mammary gland and secrete the corresponding protein into their milk.

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Author notes

  1. A. J. Clark: Corresponding author.

Authors and Affiliations

  1. AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, West Mains Road, Edinburgh, EH9 3JQ, Scotland

    A. J. Clark, P. Brown, S. Harris, M. McClenaghan, C. B. A. Whitelaw & I. Wilmut

  2. Scottish National Blood Transfusion Service, Royal Infirmary Edinburgh, EH3 9HB, Scotland

    H. Bessos, C. Prowse & J. P. Simons

  3. Department of Genetics, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JN, Scotland

    J. O. Bishop

  4. LGME-CNRS & U184-INSERM, 11 Rue Humann, 67085, Strasbourg Cedex, France

    R. Lathe

Authors
  1. A. J. Clark
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  2. H. Bessos
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  3. J. O. Bishop
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  4. P. Brown
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  5. S. Harris
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  6. R. Lathe
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  7. M. McClenaghan
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  8. C. Prowse
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  9. J. P. Simons
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  10. C. B. A. Whitelaw
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  11. I. Wilmut
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Clark, A., Bessos, H., Bishop, J. et al. Expression of Human Anti-Hemophilic Factor IX in the Milk of Transgenic Sheep. Nat Biotechnol 7, 487–492 (1989). https://doi.org/10.1038/nbt0589-487

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