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Antigen Responsive Antibody–Receptor Kinase Chimera

Bio/Technology volume 10, pages 430–433 (1992)Cite this article

Abstract

We have constructed chimeric receptors, combining murine IgM and the cytoplasmic portion of human epidermal growth factor receptor (EGFR), with the aim of developing a novel immunosensor with antigen–dependent phosphorylation activity. When intact IgM was used, the chimeric receptor showed both antigen binding and protein tyrosine kinase activity, but the kinase activity was constitutive and independent of antigen binding. However, with 1gM lacking the CH2 domain, the autophosphorylation activity increased with increasing concentrations of anti–IgM or hapten–BSA conjugate. Monovalent hapten could not induce phosphorylation but inhibited stimulation by hapten–conjugated BSA.

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Author notes

  1. Hajime Nishimura: Corresponding author.

Authors and Affiliations

  1. Department of Chemical Engineering, Faculty of Engineering, University of Tokyo, 7–3–1 Hongo, Bunkyo-ku Tokyo, 113, Japan

    Hiroshi Ueda & Hajime Nishimura

  2. Institute of Research and Innovation, 1201 Takada, Kashiwa-City, Chiba, 277, Japan

    Masako Kikuchi

  3. Tonen Fundamental Research, 1–3–1 Nishi-Tsurugaoka, Ohi-Machi, Saitama, 354, Japan

    Shintaro Yagi

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  1. Hiroshi Ueda
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  4. Hajime Nishimura
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Ueda, H., Kikuchi, M., Yagi, S. et al. Antigen Responsive Antibody–Receptor Kinase Chimera. Nat Biotechnol 10, 430–433 (1992). https://doi.org/10.1038/nbt0492-430

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  • DOI: https://doi.org/10.1038/nbt0492-430

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