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High Expression in Mammalian Cells Without Amplification

Bio/Technology volume 7, pages 359–362 (1989)Cite this article

Abstract

We have used mammalian cell lines, constructed with the expression system described, to express high levels of a heterologous gene product (human growth hormone) without recourse to gene amplification. The system employs commonly available components that combine efficiently to yield high expression. These components include the human metallothionein IIA promoter, Chinese hamster ovary cells and a protein free medium perfected for them. The system is suitable for large scale production during which growth hormone accumulates in the nutrient medium to levels above 200 mg/liter and purity of up to 90 percent.

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Author information

Author notes

  1. Jeffrey S. Friedman

    Present address: School of Medicine, Stanford University, Palo Alto, CA, 94305

Authors and Affiliations

  1. California Biotechnology Inc., 2450 Bayshore Parkway, Mountain View, CA, 94043

    Claire L. Cofer, Cheryl L. Anderson, Jake A. Kushner & Peter J. Kushner

  2. Dept. of Biotechnology, University of New South Wales, Sydney, Australia

    Peter P. Gray

  3. Garvan Institute of Medical Research, Sydney, Australia

    George E. Chapman, Margaret C. Stuart, Leslie Lazarus & John Shine

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  1. Jeffrey S. Friedman
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  2. Claire L. Cofer
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  3. Cheryl L. Anderson
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  4. Jake A. Kushner
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  5. Peter P. Gray
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  6. George E. Chapman
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  7. Margaret C. Stuart
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Friedman, J., Cofer, C., Anderson, C. et al. High Expression in Mammalian Cells Without Amplification. Nat Biotechnol 7, 359–362 (1989). https://doi.org/10.1038/nbt0489-359

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  • DOI: https://doi.org/10.1038/nbt0489-359

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