Abstract
An isolate of Bacillus thuringiensis has been identified with toxic activity against coleopteran insects but not against lepidopteran or dipteran species. Toxin crystals from this strain contain a polypeptide of approximately 64,000 molecular weight possessing insecticidal activity. This toxin differs in polypeptide composition from other reported Bacillus thuringiensis toxins and does not appear to be processed from a larger precursor form. Insecticidal activity against the Colorado potato beetle and the boll weevil suggests the possibility of commercial applications of this strain.
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References
Miller, D. 1985. Crop protection chemicals reference 1st edition, p. 500–511. Chemical and Pharmaceutical Publishing Corp, NY.
Dulmage, H.T. 1981. Insecticidal activity of isolates of Bacillus thuringiensis and their potential for pest control, p. 193–222. In: Microbial Control of Pests and Plant Diseases 1970–1980. H. D. Burgess (ed), Academic Press, NY.
Goldberg, L.J. and Margalit, J. 1977. A bacterial spore demonstrating rapid larvacidal activity against Anopheles sergentii, Uranotaenia unguiculata, Culex univitattus, Aedes aegypti, and Culex pipiens. Mosquito News 37:355–358.
Hall, I.M., Arakawa, K.Y., Dulmage, H.T., and Correa, J. 1977. The pathogenicity of strains of Bacillus thuringiensis to larvae of Aedes and to Culex mosquitos. Mosquito News 37:246–251.
Yamamoto, T. and McLaughlin, R.E. 1981. Isolation of a protein from the parasporal crystal of Bacillus thuringiensis var. kurstaki toxic to mosquito larvae Aedes taeniorhynchus. Biochem. Biophys. Res. Comm. 103:414–421.
Schnepf, H.E. and Whiteley, H.R. 1981. Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli. Proc. Nat. Acad. Sci. USA 78:2893–2897.
Klier, A., Fargette, F., Ribier, J., and Rappaport, G. 1982. Cloning and expression of the crystal protein gene from Bacillus thuringiensis strain berliner 1715. EMBO J. 1:791–799.
Adang, M.J., Staver, M.J., Rocheleau, T.A., Leighton, J., Barker, R.F., and Thompson, D.V. 1985. Characterization of full length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta. Gene 36:289–300.
Schnepf, H.E., Wong, H.C., and Whitely, H.R. 1985. The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence. J. Biol. Chem. 260:6257–6264.
Shibano, Y., Yamagata, A., Nakamura, N., Izuka, T., Sugisaki, H., and Takanami, M. 1985. Nucleotide sequence coding for the insecticidal fragment of the Bacillus thuringiensis crystal protein. Gene 34:243–251.
Krieg, A., Huger, A.M., Langenbrook, G.A., and Schnetter, W. 1983. Bacillus thuringiensis var. tenebrionis: ein neuer gegenuber Larven von Coleopteren wirksamer Pathotyp. Z. Ang. Ent. 96:500–508.
Krieg, A., Huger, A.M., Langenbruch, G.A., and Schnetter, W. 1984. Neue Ergebniss uber Bacillus thuringiensis var. tenebrionis unter besonderer Berucksichtigung seiner Wirkung auf den Kartoffelkafer (Leptinotarsa decemlineata). Ang. Schadlingskde., Pflanzenschutz, Umweltschutz. 57:145–150.
Bulla, Jr., L.A., Bechtel, D.B., Kramer, K.J., Shethna, Y.I., Aronson, A.I., and Fitz-James, P.C. 1980. Ultrastructure, physiology, and biochemistry of Bacillus thuringiensis var. kurstaki. CRC Crit. Rev. Microbiol. 8:147–204.
Finney, D.J. 1971. Probit Analysis 3d edition. Cambridge University Press, Cambridge.
Burnette, W.N. 1981. “Western Blotting”: Electrolytic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112:195–203.
Maniatis, T., Fritsch, E.F., and Sambrook, J. 1982. Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Cuatrecases, P. and Anfinsen, C.B. 1971. Affinity chromatography. In: Methods in Enzymology, Vol. 22. W.B. Jacoby (ed). Academic Press, NY.
Hanahan, D. and Meselson, M. 1980. Plasmid screening at high colony density. Gene 10:63–67.
Dulmage, H.T., et al. 1982. Bacillus thuringiensi cultures available from the U.S. Department of Agriculture. Agricultural Research Service, New Orleans.
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Herrnstadt, C., Soares, G., Wilcox, E. et al. A New Strain of Bacillus thuringiensis with Activity Against Coleopteran Insects. Nat Biotechnol 4, 305–308 (1986). https://doi.org/10.1038/nbt0486-305
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DOI: https://doi.org/10.1038/nbt0486-305