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Tomato transgene structure and silencing

Nature Biotechnology volume 23, pages 287–289 (2005)Cite this article

To the editor:

In last year's March issue (Nat. Biotechnol. 22, 267–268, 2004), Frank Rainer Schmidt provided an example of past research relevant to the current topic of RNA interference (RNAi). As a follow-up, we would like to briefly review previous work at Calgene (Davis, CA, USA; now part of Monsanto, St. Louis, MO, USA) on the use of antisense technology to silence the gene encoding tomato polygalacturonase (PG). These data were published over the period 1988–1992, and on inspection may have involved the production of double-stranded RNA (dsRNA) and mechanisms now termed RNAi or post-transcriptional gene silencing. In particular, we would like to emphasize the importance of determining transgene insert structure and the resulting transcription patterns in understanding mechanisms responsible for gene silencing.

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Figure 1: Structures of the T-DNA inserts for each of the eight selected tomato events, as determined by Southern analysis.

Bob Crimi

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  1. Monsanto Company, Calgene Campus, Davis, 95616, California, USA

    Rick A Sanders & William Hiatt

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  1. Rick A Sanders
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  2. William Hiatt
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Correspondence to William Hiatt.

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Sanders, R., Hiatt, W. Tomato transgene structure and silencing. Nat Biotechnol 23, 287–289 (2005). https://doi.org/10.1038/nbt0305-287b

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