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Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis

Bio/Technology volume 11, pages 194–200 (1993)Cite this article

Abstract

We introduced a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis into immature embryos of an elite line of maize using microprojectile bombardment. This gene was expressed using either the CaMV 35S promoter or a combination of two tissue specific promoters derived from maize. High levels of CryIA(b) protein were obtained using both promoter configurations. Hybrid maize plants resulting from crosses of transgenic elite inbred plants with commercial inbred lines were evaluated for resistance to European corn borer under field conditions. Plants expressing high levels of the insecticidal protein exhibited excellent resistance to repeated heavy infestations of this pest.

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Author notes

  1. Michael G. Koziel: Corresponding author.

Authors and Affiliations

  1. CIBA-GEIGY Agricultural Biotechnology Research Unit, Research Triangle Park, NC, 27709

    Michael G. Koziel, Cindy Bowman, Nadine B. Carozzi, Rebecca Crenshaw, Lyle Crossland, John Dawson, Nalini Desai, Martha Hill, Sue Kadwell, Karen Launis, Kelly Lewis, Daryl Maddox, Kathryn McPherson, Ellis Merlin, Richard Rhodes, Gregory W. Warren, Martha Wright & Stephen V. Evola

  2. CIBA-GEIGY Seeds, 1301 West Washington, Bloomington, IL, 61702

    Gary L. Beland & Moez R. Meghji

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  1. Michael G. Koziel
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Koziel, M., Beland, G., Bowman, C. et al. Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis. Nat Biotechnol 11, 194–200 (1993). https://doi.org/10.1038/nbt0293-194

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