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Saccharomyces Cerevisiae Cells Secreting an Aspergillus Niger β-Galactosidase Grow on Whey Permeate

Bio/Technology volume 10, pages 82–85 (1992)Cite this article

Abstract

We describe the construction of a lactose-utilizing Saccharomyces cerevisiae that expresses the cDNA for a secreted, thermostable β-galactosidase (lacA) from Aspergillus niger. Yeast cells expressing the lacA gene from the yeast ADH1 promoter on a multicopy plasmid secrete up to 40% of the total P-galactosidase activity into the growth medium. The secreted product is extensively N-glycosylated, and cells expressing the lacA gene grow on whey permeate (4% w/v lactose) with a doubling time of 1.6 hours. Such strains may offer a solution to the increasing problem of waste whey disposal.

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Authors and Affiliations

  1. Centre For Biotechnology, Imperial College of Science and Technology, London, SW7 2AZ, U.K.

    Vijay Kumar, Sundaram Ramakrishnan & Brian S. Hartley

  2. V.T.T Biotechnical Laboratory, P.O. Box 202, SF-02151, Espoo, Finland

    Tuula T. Teeri

  3. Glaxo Institute of Molecular Biology, 14 Chemin des Anlx, Case Postale 674, 1228 Plan-les-Ouates, Geneva, Switzerland

    Jonathan K. C. Knowles

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  1. Vijay Kumar
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  2. Sundaram Ramakrishnan
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  3. Tuula T. Teeri
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  4. Jonathan K. C. Knowles
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  5. Brian S. Hartley
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Kumar, V., Ramakrishnan, S., Teeri, T. et al. Saccharomyces Cerevisiae Cells Secreting an Aspergillus Niger β-Galactosidase Grow on Whey Permeate. Nat Biotechnol 10, 82–85 (1992). https://doi.org/10.1038/nbt0192-82

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  • DOI: https://doi.org/10.1038/nbt0192-82

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