Abstract

Understanding the biology that underlies histologically similar but molecularly distinct subgroups of cancer has proven difficult because their defining genetic alterations are often numerous, and the cellular origins of most cancers remain unknown1,2,3. We sought to decipher this heterogeneity by integrating matched genetic alterations and candidate cells of origin to generate accurate disease models. First, we identified subgroups of human ependymoma, a form of neural tumour that arises throughout the central nervous system (CNS). Subgroup-specific alterations included amplifications and homozygous deletions of genes not yet implicated in ependymoma. To select cellular compartments most likely to give rise to subgroups of ependymoma, we matched the transcriptomes of human tumours to those of mouse neural stem cells (NSCs), isolated from different regions of the CNS at different developmental stages, with an intact or deleted Ink4a/Arf locus (that encodes Cdkn2a and b). The transcriptome of human supratentorial ependymomas with amplified EPHB2 and deleted INK4A/ARF matched only that of embryonic cerebral Ink4a/Arf−/− NSCs. Notably, activation of Ephb2 signalling in these, but not other, NSCs generated the first mouse model of ependymoma, which is highly penetrant and accurately models the histology and transcriptome of one subgroup of human supratentorial tumour. Further, comparative analysis of matched mouse and human tumours revealed selective deregulation in the expression and copy number of genes that control synaptogenesis, pinpointing disruption of this pathway as a critical event in the production of this ependymoma subgroup. Our data demonstrate the power of cross-species genomics to meticulously match subgroup-specific driver mutations with cellular compartments to model and interrogate cancer subgroups.

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Accessions

Primary accessions

Gene Expression Omnibus

Data deposits

The microarray data generated during this study has been deposited in GEO under the accession number GSE21687.

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Acknowledgements

R.J.G. holds the Howard C. Schott Research Chair from the Malia’s Cord Foundation, and is supported by grants from the National Institutes of Health (R01CA129541, P01CA96832 and P30CA021765), the Collaborative Ependymoma Research Network (CERN) and by the American Lebanese Syrian Associated Charities (ALSAC). K.D.W. is supported by NRSA Training Grant T32 CA070089. We are grateful to N. Heintz for providing the Blbp–eGFP mouse, T. Pawson for providing mouse Ephb2 cDNA, M. Roussel for the pCX4-IRES-RFP virus, J. Downing and M. Relling for access to 500K SNP profiles of normal human DNA, and the staff of the Hartwell center for Bioinformatics and Biotechnology, Vector Production Core, ARC, AIC and Cell and Tissue Imaging at St Jude Children’s Research Hospital for technical assistance.

Author information

Author notes

    • Robert A. Johnson
    •  & Karen D. Wright

    These authors contributed equally to this work.

Affiliations

  1. Department of Developmental Neurobiology, St Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA

    • Robert A. Johnson
    • , Karen D. Wright
    • , Helen Poppleton
    • , Kumarasamypet M. Mohankumar
    • , Elsie White
    • , Christopher Eden
    • , Twala Hogg
    • , Jennifer Atkinson
    • , Tanya A. Kranenburg
    •  & Richard J. Gilbertson
  2. Department of Oncology, St Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA

    • Karen D. Wright
    • , Mariko DeWire
    • , Amar Gajjar
    •  & Richard J. Gilbertson
  3. Hartwell Centre for Bioinformatics and Biotechnology, St Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA

    • David Finkelstein
    • , Geoffrey Neale
    •  & Yong-Dong Wang
  4. Department of Biostatistics, St Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA

    • Stanley B. Pounds
  5. Northern Institute for Cancer Research, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, UK

    • Vikki Rand
  6. Hematology-Oncology, Seattle Children's Hospital, Seattle, Washington 98105, USA

    • Sarah E. S. Leary
  7. Division of Neurosurgery, Arthur and Sonia Labatt Brain Tumour Research Centre, Hospital for Sick Children, Toronto, Ontario M4N 1X8, Canada

    • Paul Northcott
    • , Stephen Mack
    •  & Michael D. Taylor
  8. Children’s Brain Tumour Research Centre, University of Nottingham, Nottingham NG7 2RD, UK

    • Beth Coyle
    •  & Richard G. Grundy
  9. Department of Surgery, University of Alabama, 1900 University Boulevard, Birmingham, Alabama 35294, USA

    • Yancey Gillespie
  10. New York University, Langone Medical Center, 550 First Avenue, New York, New York 10016, USA

    • Jeffrey C. Allen
  11. Department of Radiological Sciences, St Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA

    • Thomas Merchant
  12. Department of Surgery, St Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA

    • Fredrick A. Boop
    •  & Robert. A. Sanford
  13. Department of Pathology, St Jude Children’s Research Hospital, 262 Danny Thomas Place, Memphis, Tennessee 38105, USA

    • David W. Ellison

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Contributions

R.J.G., R.G.G. and M.D.T. conceived the research and planned experiments. R.A.J. and K.D.W. also planned and conducted the great majority of the experiments under the direction of R.J.G.; A.G., Y.G., J.C.A., M.D.T. and R.G.G. provided human tumour material. All authors contributed experimental expertise and participated in the writing of the manuscript. D.F., S.B.P., Y.-D.W. and G.N. provided bioinformatic expertise. D.W.E. provided pathology review and iFISH analysis.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Richard J. Gilbertson.

Supplementary information

PDF files

  1. 1.

    Supplementary Information

    This file contains Supplementary Methods, References, Supplementary Figures 1-6 with legends, Supplementary Table legends 1-6 (see pages 32-33) and Supplementary Tables 1-6.

  2. 2.

    Supplementary Information

    This file contains gene cards, which contain a comprehensive summary of the DNA alteration and gene expression within each validated focal amplification and deletion identified in Figure 1 of the main paper.

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