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Molecular basis of the copulatory plug polymorphism in Caenorhabditis elegans

Nature 454, 1019–1022 (21 August 2008) | Download Citation

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Abstract

Heritable variation is the raw material for evolutionary change, and understanding its genetic basis is one of the central problems in modern biology. We investigated the genetic basis of a classic phenotypic dimorphism in the nematode Caenorhabditis elegans. Males from many natural isolates deposit a copulatory plug after mating, whereas males from other natural isolates?including the standard wild-type strain (N2 Bristol) that is used in most research laboratories?do not deposit plugs1. The copulatory plug is a gelatinous mass that covers the hermaphrodite vulva, and its deposition decreases the mating success of subsequent males2. We show that the plugging polymorphism results from the insertion of a retrotransposon into an exon of a novel mucin-like gene, plg-1, whose product is a major structural component of the copulatory plug. The gene is expressed in a subset of secretory cells of the male somatic gonad, and its loss has no evident effects beyond the loss of male mate-guarding. Although C. elegans descends from an obligate-outcrossing, male?female ancestor3,4, it occurs primarily as self-fertilizing hermaphrodites5,6,7. The reduced selection on male?male competition associated with the origin of hermaphroditism may have permitted the global spread of a loss-of-function mutation with restricted pleiotropy.

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Acknowledgements

We thank the Caenorhabditis Genetics Center, M. Ailion, E. Dolgin, A. Barrière, M.-A. Félix and P. McGrath for strains and reagents. Work at Bowdoin College was supported by college funds, by the NIH (grant P20 RR-016463 from the INBRE Program of the National Center for Research Resources), the NSF (grant 0110994), and by an award to Bowdoin College by the Howard Hughes Medical Institute under the Undergraduate Science Education Program. Work at Princeton University was supported by grants from the NIH (R01 HG004321 to L.K. and P50 GM071508 to the Lewis-Sigler Institute), a James S. McDonnell Foundation Centennial Fellowship (L.K.), and a Jane Coffin Childs Fellowship (M.V.R.). We thank S. Civillico for assistance in the laboratory, F. Hagen for experimental advice, and E. Anderson, H. Coller, L. Gordon and H. Seidel for comments on the manuscript.

Author information

    • Michael F. Palopoli
    •  & Matthew V. Rockman

    These authors contributed equally to this work.

Affiliations

  1. Department of Biology, Bowdoin College, 6500 College Station, Brunswick, Maine 04011, USA

    • Michael F. Palopoli
    • , Aye TinMaung
    • , Camden Ramsay
    • , Stephen Curwen
    • , Andrea Aduna
    •  & Jason Laurita

  2. Lewis-Sigler Institute for Integrative Genomics and Department of Ecology and Evolutionary Biology, Carl Icahn Laboratory, Princeton University, Princeton, New Jersey 08544, USA

    • Matthew V. Rockman
    •  & Leonid Kruglyak

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Corresponding author

Correspondence to Michael F. Palopoli.

Supplementary information

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    Supplementary Information 1

    The file contains Supplementary Methods and Supplementary Figures S1-S5 with Legends.

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    Supplementary Information 2

    The file contains Supplementary Movie 1. Three-dimensional reconstruction of a stack of confocal images of an F1 male from a cross between CB4856 and QX1193, which carries the integrated plg-1::GFP transgene qqIs1.

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    Supplementary Information 3

    The file contains Supplementary Table 1 including natural isolate strains, localities, phenotypes, and genotypes.

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DOI

https://doi.org/10.1038/nature07171

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