Abstract
Hyaline vascular Castleman disease is traditionally regarded as a reactive hyperplastic process. Occasional cases, however, have been reported with cytogenetic anomalies bringing this concept into question. In this study, we used conventional and methylation-specific polymerase chain reaction methods to assess the human androgen receptor α (HUMARA) gene in 29 female patients with hyaline vascular Castleman disease and compared the results with three cases of plasma cell Castleman disease and 20 cases of age-matched lymphoid hyperplasia. We also assessed for immunoglobulin gene and T-cell receptor gene rearrangements, and conventional cytogenetic analysis was performed in three cases of hyaline vascular Castleman disease. In cases with informative results, conventional and methylation-specific human androgen receptor α gene analyses yielded a monoclonal pattern in 10 of 19 (53%) and 17 of 23 (74%) cases of hyaline vascular Castleman disease, respectively. A monoclonal pattern was also detected in three cases of plasma cell Castleman disease but not in cases of lymphoid hyperplasia. The frequency of monoclonality was higher for lesions >5 cm in size (100%) and for the stromal-rich variant (91%). Cytogenetic abnormalities in stromal cells were revealed in two cases of hyaline vascular Castleman disease and no cases showed monoclonal immunoglobulin or T-cell receptor gene rearrangements. Follow-up data showed persistent disease in 4 of 23 (17%) patients. We conclude that hyaline vascular Castleman disease is often a monoclonal proliferation, most likely of lymph node stromal cells.
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In 1956, Castleman et al1 described the hyaline vascular variant of Castleman disease as a large, benign, asymptomatic mass involving mediastinal lymph nodes. Castleman disease has also been designated as ‘angiofollicular lymph node hyperplasia’ or ‘giant cell lymph node hyperplasia.’ Subsequently, Castleman and co-workers2 expanded the concept to include plasma cell variant cases and others recognized multicentric disease and the role of human herpes virus 8 (HHV-8) in some cases.3, 4, 5 Recent studies have focused on the pathogenetic role of overexpression of interleukin-6, a B-lymphocyte stimulator, and subsequent plasma cell proliferations in plasma-cell-type and multicentric Castleman disease.6, 7 However, the etiology of hyaline vascular variant remains unclear and there are limited studies regarding this issue.
Hyaline vascular Castleman disease, the most common form of Castleman disease, is characterized clinically by a solitary, slow-growing mass, most typically in the mediastinum or neck, without associated constitutional symptoms.8 The histological features characteristic of hyaline vascular Castleman disease are abnormal, lymphocyte-depleted follicles with sclerotic radial arterioles, vessel-rich interfollicular tissues, and compressed or absent subcapsular sinuses. Hyaline vascular Castleman disease is traditionally regarded as a hyperplastic or reactive process and surgical excision is adequate for treatment for most patients.5, 9
In addition to characteristic follicular changes, overgrowth of stromal cells in hyaline vascular Castleman disease is a well-documented phenomenon.10 Although stromal overgrowth is usually considered secondary, several authors have stressed the possible pathogenetic role of dysplastic follicular dendritic cells in this disorder.11, 12, 13 Furthermore, there are emerging data demonstrating clonal cytogenetic abnormalities in stromal cells of hyaline vascular Castleman disease without overt stromal cell overgrowth. Pauwels et al14 reported a case of hyaline vascular Castleman disease with dysplastic follicular dendritic cells associated with a clonal t(1;16)(p11; p11).14 Cokelaere et al15 described another case of hyaline vascular Castleman disease and showed HMGIC gene rearrangement in follicular dendritic cells of the lesion by combined immunofluorescence and FISH analysis. In a previous report of hyaline vascular Castleman disease, we showed stromal cells that had features of myoid cells in short-term culture associated with a clonal t(1;22)(p22;q13).16 The finding of genetic anomalies in hyaline vascular Castleman disease suggests that it is a monoclonal proliferation.
In this study, we have analyzed the human androgen receptor α (HUMARA) gene in female patients with hyaline vascular Castleman disease to assess for monoclonality. Our results suggest that hyaline vascular Castleman disease is commonly a monoclonal lesion, most likely arising in lymph node stromal cells.
Materials and methods
Female Cases of Hyaline Vascular Castleman Disease
Female patients with hyaline vascular Castleman disease were enrolled from the archival files of National Cheng Kung University Hospital, Taiwan (N1–N12), the MD Anderson Cancer Center, USA (M1–M7), and Chang Gung Memorial Hospital-Kaohsiung Medical Center (C1–C13). Studies were carried out under a laboratory protocol approved by our institutional review board (NCKUH-BR-99-116-C) and were in accordance with the Declaration of Helsinki of 1975, as revised in 1983. The diagnosis was reviewed by three hematopathologists and the hyaline vascular Castleman disease cases were further subclassified as classical, lymphoid,8 and stromal-rich variant.9, 11 The classical cases of hyaline vascular Castleman disease showed regressed and confluent follicles with radially penetrating sclerotic arterioles (‘lollipop lesion’), hyaline deposits in germinal centers, concentric layering of mantle zone lymphocytes (‘onion skin appearance’), inapparent sinuses, and interfollicular vascular hyperplasia (Figures 1a and b). The lymphoid variant was characterized by mantle zone expansion around the atrophic, inconspicuous germinal center that may mimic follicular lymphoma or mantle cell lymphoma (Figure 1c). The stromal-rich variant was defined as a prominent interfollicular zone occupying 50% or more of the nodal area.11 The interfollicular zones had increased stromal cells and blood vessels and were associated with residual lymphoid follicles (Figure 1d). The frequent components of stromal cells were actin-positive ‘myoid’ cells and follicular dendritic cells. No clinical evidence of IgG4-related diseases or lymphoma association was noted. For comparison, three cases of plasma-cell-type Castleman disease (N8, M2, and C4) and 20 cases of lymphoid hyperplasia (LH1–LH20) were included. All specimens were fixed in 10% neutral formalin and embedded in paraffin. Clinical data and follow-up information were obtained by chart review.
Histological and Immunohistochemical Stains
Hematoxylin and eosin (H&E) stain and immunohistochemical (IHC) studies were performed on formalin-fixed, paraffin-embedded tissue sections. IHC analysis was performed on 4-μm-thick sections with a mouse anti-HHV-8 (1:50 dilution; Novocastra, Leica Biosystems Newcastle, Melbourne, Australia) as the primary antibody after tissues were deparaffinized with xylene and pretreated with the Epitope Retrieval Solution 2 (EDTA, pH 9.0). The procedures were carried out with the Bond-Max Automated IHC stainer (Leica Biosystems Newcastle). Counterstaining was carried out with hematoxylin.
Conventional HUMARA Gene Assay and HUMARA Methylation-Specific Polymerase Chain Reaction
Genomic DNA from ten 8-μm-thick sections of paraffin-embedded, formalin-fixed tissues was isolated with the QIAamp genomic DNA kit (Qiagen, Studio City, CA, USA) according to the manufacturer’s instructions. Only tissues involved by Castleman disease were sectioned. Erdheim–Chester disease and peripheral blood mononuclear cells were used as positive and negative controls, respectively, as our previous study.17 The clonality analysis was performed by conventional HUMARA and methylation-specific polymerase chain reaction (PCR) HUMARA.
For conventional HUMARA,17, 18 aliquots of 25 ng of HhaI-digested or -undigested genomic DNA were amplified with primers (HUMARA 1A, 5′-AGGTGGAAGATTCAGCCAAG-3′; and HUMARA 1B, 5′-TGTGAAGGTTGCTGTTCCTC-3′) in a 20 μl of PCR mixture. All samples were digested with HhaI for at least 3 h. The XE169 gene fold reduction was measured to monitor the efficiency of restriction enzyme digestion as described previously.19 After 10 min of denaturation at 95 °C, PCRs were then perfomed using the following conditions for 45 cycles: 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s. PCR products were electrophoresed on a 11% acrylamide gel and visulaized by staining with ethidium bromide.
For methylation-specific PCR HUMARA analysis,20 500 ng of DNA was modified with sodium bisulfate by EpiTect Fast DNA Bisulfide kit (Qiagen) according to the manufacturer’s instructions. Aliquots of 25 ng of modified DNA were subjected to PCR in a total of 20 μl mixture with primers specific for unmethylated (U: US2, 5′-GGTTGTGAGTGTAGTATTTTTTGGT-3′; and HUMARA 1B) or methylated (M: MS2, 5′-CGAGCGTAGTATTTTTCGGC-3′; and HUMARA 1B) DNA. After denaturation at 95 °C for 10 min, PCRs were perfomed using the following conditions for 45 cycles: 95 °C for 45 s, 55 °C for 45 s, and 72 °C for 60 s. PCR products of methylation-specific HUMARA were analyzed on a 1.8% agarose gel and visualized by staining with ethidium bromide.
PCR Studies for B- and T-Cell Clonality
To detect possible monoclonal proliferations of lymphoid cells, the BIOMED-2 protocols were performed from paraffin blocks for the rearrangement of immunoglobulin heavy- and light-chain genes and the T-cell receptor γ and β genes, as described in our previous study.21
Conventional Cytogenetic Analysis
Conventional cytogenetic analysis was performed in three cases (case N5 and two additional males) of hyaline vascular Castleman disease to test any cytogenetic abnormalities. All the three cases showed no evidence of stromal overgrowth. The G-banding method was used and two cases had been reported previously.16
Statistical Analysis
Appropriate statistical tests were used to examine the relationships and correlations between variables, including Student t-test, and χ2-test or Fisher’s exact test. The P-value referred to is two-sided. The analyses were carried out using SPSS 13.0 statistical software (SPSS, Chicago, IL, USA).
Results
Female Cases of Hyaline Vascular Castleman Disease
The study group included 29 patients with hyaline vascular Castleman disease (Table 1) with a mean age of 32.8 years (range, 5–65 years). All cases received total or partial excision. These patients were followed for a mean of 40 months (range, 0.4–120.5 months). All 29 cases of hyaline vascular Castleman disease as well as a comparison group of three cases of plasma cell Castleman disease were negative for immunostaining of HHV-8 latent nuclear antigen. Persistent disease was found in 4 of 23 (17%) cases of hyaline vascular Castleman disease (Table 2).
HUMARA Gene Analysis and Immunoglobulin and T-Cell Receptor Gene Rearrangement
The results are listed in Table 1. In hyaline vascular Castleman diseases with informative results, conventional HUMARA analysis (Figure 2a) showed a monoclonal pattern in 10 of 19 (53%) cases, and methylation-specific PCR HUMARA analysis (Figure 2b) yielded a monoclonal pattern in 17 of 23 (74%) cases. The overall positive rate for monoclonality was 79% (22/28) (Table 2). The monoclonal rates for three variants of hyaline vascular Castleman disease were: classical 69%, lymphoid 75%, and stromal-rich 91%. Interestingly, along with the incremental increase in the frequency of monoclonality, the mean age for the three variants of hyaline vascular Castleman disease was also increased: classical 30.6 years, lymphoid 32.3 years and stromal-rich 35.4 years (Table 2). Although the monoclonal rate was higher for lesions in older patients, in central lymph nodes, and with stromal proliferations (cases N7, M3, and M7), only larger lesion size (>5 cm) showed statistical significance (P=0.034; Table 2). In comparison, all LH cases with informative results showed a polyclonal pattern of HUMARA gene (Supplementary Table S1 and Supplementary Figure S1).
In contrast to the frequent monoclonality for HUMARA gene, all 29 cases of hyaline vascular Castleman disease were negative for monoclonal immunoglobulin and T-cell receptor gene rearrangements (Table 1). One case of plasma cell Castleman disease (C4) was positive for monoclonal immunoglobulin gene rearrangements (Supplementary Figure S2). The other two cases lacked immunoglobulin gene rearrangements and all three were negative for T-cell receptor gene rearrangements. All LH cases were negative for B- and T-cell clonality except one (LH10) being equivocal for immunoglobulin gene rearrangement (Supplementary Table S1).
Conventional Cytogenetic Analysis
Conventional cytogenetic studies were carried out for three cases (N5 and two male patients, 39 and 45 years), which showed no evidence of stromal overgrowth. Two of them showed cytogenetic abnormalities with t(1;22)(p22;q13) and t(7;8)(qter;q12) in each (Figure 3).
Discussion
In this study, we provide evidence that most cases of hyaline vascular Castleman disease are monoclonal when assessed by HUMARA gene analysis, but no cases had monoclonal immunoglobulin or T-cell receptor gene rearrangements. Interestingly, this monoclonal HUMARA gene pattern was more frequently found in larger lesions (>5 cm), in stromal-rich variant, and in lesions with stromal overproliferations. In addition, two cases without stromal overgrowth carried cytogenetic anomalies. These findings suggest that hyaline vascular Castleman disease is a monoclonal proliferative lesion. Although monoclonality is not equal to neoplasia, it is an early event in the multistep neoplastic process,19 and therefore the findings suggest that hyaline vascular Castleman disease is a benign neoplasm, which has been favored by few authors.22, 23 This suggestion is in contrast to traditional ideas about the pathogenesis of hyaline vascular Castleman disease, which is largely unknown but regarded to be most likely hyperplastic or hamartomatous in nature.9, 12
Hyaline vascular Castleman diseases with genetic anomalies have been reported previously (Supplementary Table S2) and the cell origin responsible for the abnormal genetics has been demonstrated directly or indirectly to be stromal cells, either follicular dendritic cells or actin-positive myoid cells.14, 15, 16 Hyaline vascular Castleman disease is composed of proliferating lymphoid cells and stromal cells. Provided that it is monoclonal and neoplastic, the tumor cells would be lymphoid or stromal cells. As the immunoglobulin and T-cell receptor genes are in germline status, it indicates that the stromal cells would be the culprit. In support of this, subsequent development of stromal tumors has been repeatedly reported in hyaline vascular Castleman disease, usually originating from follicular dendritic cells, vascular cells, myoid cells, and fibrohistiocytic cells.5, 10, 24, 25, 26, 27 Furthermore, hyaline vascular Castleman disease is well known to be associated with paraneoplastic pemphigus, a syndrome typically occurring in tumor patients.28, 29, 30 It appears that hyaline vascular Castleman disease shares some neoplastic features.
The histogenesis of stromal overgrowth in hyaline vascular Castleman disease is commonly considered secondary.10 However, the findings of genetic changes preceding the morphologically stromal overgrowth may elicit the idea that the genetic alteration of stromal cells is a leading event in hyaline vascular Castleman disease. The compressed sinuses are a characteristic feature of hyaline vascular Castleman disease and are probably related to stromal proliferation. The compressed sinuses result in the lymph node being a closed system that sequesters soluble factors or cytokines in the lymph node that could subsequently lead to overgrowth of stromal cells.31, 32, 33 As hyaline vascular Castleman diseases of larger size and with stromal overgrowth were monoclonal in almost all cases, it indicates that the monoclonal proliferation of stromal cells become more prominent with time.
The HUMARA gene analysis is the most widely used method for the determination of X-chromosome inactivation, which has been the standard method to assay the clonal nature for lesions in female patients.19 In addition to the conventional HUMARA gene analysis described by Allen et al,18 some other methods have also been reported, such as the methylation-specific PCR HUMARA assay.20 This assay can minimize the false polyclonality resulting from incomplete DNA digestion by methylation-sensitive restriction enzymes. Accordingly, we found that methylation-specific PCR HUMARA assay increased the monoclonal rate by 21%. The remaining cases with non-informative results may result from the degradation of nucleic acids or effects of fixatives in paraffin-embedded tissue.19, 34 We have monitored the efficiency of HhaI enzyme digestion and ensured the validity of our results by measuring XE169 gene amplification, as described previously (Supplementary Figure S3).19
In conclusion, our results show that most cases of hyaline vascular Castleman disease are monoclonal proliferations, most likely of stromal cell origin, as the lymphoid cells are polyclonal. Some cases are associated with conventional cytogenetic anomalies. On the basis of the well-known clinical behavior of patients with hyaline vascular Castleman disease, this lesion is therefore a benign neoplasm of lymph node stromal cells, although rare cases can undergo progression to neoplastic stromal cell neoplasms. We believe our study has shed light on the pathogenesis of hyaline vascular Castleman disease.
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Acknowledgements
This study was supported by grants from the Taiwan National Science Council (NSC100-2320-B-006-005-MY3) and Department of Health, Executive Yuan, Taiwan (DOH101-TD-C-111-003: to establish centers of excellence for cancer research in Taiwan) to KC Chang, and a grant from the National Cheng Kung University Hospital, Taiwan (NCKUH-10002020) to LY Kao. This study was presented in poster format at the United States and Canadian Academy of Pathology (USCAP) 2012 Annual Meeting, 17–23 March 2012, Vancouver, BC, Canada.
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Chang, KC., Wang, YC., Hung, LY. et al. Monoclonality and cytogenetic abnormalities in hyaline vascular Castleman disease. Mod Pathol 27, 823–831 (2014). https://doi.org/10.1038/modpathol.2013.202
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DOI: https://doi.org/10.1038/modpathol.2013.202
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