To the Editor: With interest we read the article of Lopez-Collazo et al.1 The authors reported about the in vivo attenuation and evolution of methicillin-resistant Staphylococcus aureus (MRSA) isolates, which were recovered from an individual cystic fibrosis (CF) patient 13 years apart. The authors described that these isolates displayed a “slow growth”. Furthermore, in this article, Figure 3 shows the colony appearance of the first MRSA isolate revealing very small colonies. Both the slow growth and the small colonies mentioned by the authors are typical features of small colony variants (SCVs).
We recently showed that S. aureus isolates with a SCV phenotype and thymidine dependency are attenuated in virulence and showed dramatically changed metabolism.2 Although SCVs are associated with persistent and recurrent S. aureus infections such as osteomyelitis and device-related infections,3 especially thymidine-dependent SCVs can often be retrieved from the airways of CF patients and are associated with worse lung disease.4 Many studies pointed out that SCVs originate from normal S. aureus phenotypes usually in cases of long-lasting infections and that SCVs may occur and be selected because of their optimized persistence properties.3
Therefore, it would be of interest to know whether this patient was colonized/infected by normal S. aureus phenotypes earlier. As the patient was 9 years when the first MRSA-SCV was collected, it is very likely that normal S. aureus isolates were already present earlier. A comparison of such a normal strain to the latest isolate recovered in 2009 would be even more pronounced, not only in terms of the bacterial adaptation but also in terms of the immune response of the patient. It has been shown that SCVs escape the immune response by downregulation of important virulence genes,5 which is in line with the authors’ observations. Furthermore, a detailed analysis of the whole genome sequencing data of the two SCV isolates would be of major interest. One could assume that the strains carry defined mutations, which might cause the SCV phenotype (e.g., mutations in heme, menadione or thymidine pathways).3 However, such mutations can only be retrieved if these SCV isolates are compared with a normal isogenic S. aureus isolate recovered from the patient or compared with another normal closely related strain.
Moreover, it would be of interest to determine the auxotrophism of these SCVs, as a mean to investigate which substrate will support the phenotypic reversion of the SCVs to determine the underlying mechanism.
References
Lopez-Collazo, E. et al. In vivo attenuation and genetic evolution of a ST247-SCCmecI MRSA clone after 13 years of pathogenic bronchopulmonary colonization in a patient with cystic fibrosis: implications of the innate immune response. Mucosal Immunol doi:10.1038/mi.2014.73[Epub ahead of print] (2014).
Kriegeskorte, A. et al. Inactivation of thyA in Staphylococcus aureus attenuates virulence and has a strong impact on metabolism and virulence gene expression. mBio 5, e01447-14 (2014).
Proctor, R.A. et al. Small colony variants: a pathogenic form of bacteria that facilitates persistent and recurrent infections. Nat. Rev. Microbiol. 4, 295–305 (2006).
Wolter, D.J. et al. Staphylococcus aureus small-colony variants are independently associated with worse lung disease in children with cystic fibrosis. Clin. Infect. Dis. 57, 384–391 (2013).
Tuchscherr, L. et al. Staphylococcus aureus phenotype switching: an effective bacterial strategy to escape host immune response and establish a chronic infection. EMBO Mol. Med 3, 129–141 (2011).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kriegeskorte, A., Block, D. & Kahl, B. Response to “In vivo attenuation and genetic evolution of a ST247-SCCmecI MRSA clone after 13 years of pathogenic bronchopulmonary colonization in a patient with cystic fibrosis: implications of the innate immune response”. Mucosal Immunol 8, 696 (2015). https://doi.org/10.1038/mi.2015.9
Published:
Issue Date:
DOI: https://doi.org/10.1038/mi.2015.9