High-dimensional immune profiling of total and rotavirus VP6-specific intestinal and circulating B cells by mass cytometry

Nair, N; Newell, E W; Vollmers, C; Quake, S R; Morton, J M; Davis, M M; He, X S; Greenberg, H B

doi:10.1038/mi.2015.36

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Published: 22 April 2015

High-dimensional immune profiling of total and rotavirus VP6-specific intestinal and circulating B cells by mass cytometry

N Nair^1,2,
E W Newell^2,3,
C Vollmers⁴,
S R Quake⁴,
J M Morton⁵,
M M Davis^2,6,7,
X S He^1,2 &
…
H B Greenberg^1,2

Mucosal Immunology volume 9, pages 68–82 (2016)Cite this article

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Abstract

In-depth phenotyping of human intestinal antibody secreting cells (ASCs) and their precursors is important for developing improved mucosal vaccines. We used single-cell mass cytometry to simultaneously analyze 34 differentiation and trafficking markers on intestinal and circulating B cells. In addition, we labeled rotavirus (RV) double-layered particles with a metal isotope and characterized B cells specific to the RV VP6 major structural protein. We describe the heterogeneity of the intestinal B-cell compartment, dominated by ASCs with some phenotypic and transcriptional characteristics of long-lived plasma cells. Using principal component analysis, we visualized the phenotypic relationships between major B-cell subsets in the intestine and blood, and revealed that IgM⁺ memory B cells (MBCs) and naive B cells were phenotypically related as were CD27⁻ MBCs and switched MBCs. ASCs in the intestine and blood were highly clonally related, but associated with distinct trajectories of phenotypic development. VP6-specific B cells were present among diverse B-cell subsets in immune donors, including naive B cells, with phenotypes representative of the overall B-cell pool. These data provide a high dimensional view of intestinal B cells and the determinants regulating humoral memory to a ubiquitous, mucosal pathogen at steady-state.

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Introduction

Diarrheal diseases are a leading cause of morbidity and mortality in infants <5 years of age in developing countries.¹ Protective immunity against many intestinal pathogens is mediated by local antibody-secreting cells (ASCs) through the production of secretory antibodies (Abs).² As several oral vaccines that mediate protection via local induction of Abs are less effective in developing countries,³ in-depth phenotypic characterization of intestinal ASCs, and their precursors, in healthy donors is an important initial step for the development of improved vaccines.

Among the causative agents of diarrheal disease, rotaviruses (RV) are the leading cause of severe pediatric gastroenteritis worldwide.⁴ Two safe and effective oral RV vaccines have been licensed; however, the immunologic basis for the efficacy of these vaccines is unknown. Neutralizing antibodies to RV target the two viral surface proteins, VP4 and VP7. In addition, the major capsid protein, VP6, elicits a dominant Ab response post infection. VP6-specific Abs do not neutralize RV in vitro but some can inhibit RV replication intracellularly⁵ and prevent or resolve RV infection in a mouse model.⁶ Furthermore, single-chain VP6-specific Abs exhibit neutralizing activity in vitro and can confer protection against RV-induced diarrhea in vivo.⁷

The role of VP6 Abs in mediating protection in humans is not known. In peripheral blood, VP6-binding reactivity is detected at steady state in healthy donors in switched memory B cells (MBCs), IgM⁺ MBCs, CD27^- MBCs and naive B cells.^{8, 9, 10} The interaction of VP6 with surface Ig on naive B cells has been reported in adult and cord blood at a frequency of ∼2% of naive B cells.⁹ VP6-specific naive B cells exhibit distinct VH1-46 immunoglobulin gene segment bias in infant and adult repertoires,^{11, 12} whereas somatic mutations of non-dominant clones is characteristic of the VP6-specific MBC repertoire.¹⁰ IgM⁺ MBCs specific to VP6 are capable of switching to IgG in vitro and in vivo and mediate antiviral effects in vivo.¹³ VP6-specific intestinal plasma cells have also been detected at steady state in healthy adult donors and have been used to generate monoclonal Abs;¹⁴ however, comprehensive phenotypic characterization of VP6-specific intestinal B cells has not been reported.

Mass cytometry (CyTOF) is an advanced technology in multi-dimensional analysis of protein expression that facilitates multiparametric immune phenotyping of individual cells. The mass cytometry platform relies on the use of antibodies tagged with stable metal isotopes that are used to stain cells, which in turn are analyzed by a time of flight mass spectrometer.^{15, 16} The use of metal isotope-tagged antibodies circumvents problems of spectral overlap inherent in fluorescence-based cytometry, and offers an increase in the number of measurable parameters (∼40) per individual cell, while obviating the need to perform compensation across channels.^{15, 16} Multiparametric phenotypic measurements at single-cell resolution facilitate high-dimensional analysis and visualization of relationships between lymphocyte subsets in continuous development^{17, 18} rather than as a static characterization of the overall cellular pool, as has been reported for signaling in human hematopoiesis,¹⁵ CD8 T-cell diversity^{19, 20, 21} and B-cell lymphopoieisis.²²

We used mass cytometry for high-dimensional phenotypic analysis of the human small intestinal B-cell compartment by simultaneously measuring the expression of 34 markers specific for B-cell differentiation and mucosal trafficking on individual B cells in the intestine and blood of healthy adults. Using principal component analysis (PCA) to analyze high-dimensional mass cytometry data, we visualized the phenotypic relationships between major B-cell subsets, and revealed that IgM⁺ MBCs and naive B cells were phenotypically closely related as were CD27^- MBCs and switched MBCs. We demonstrate that intestinal and circulating ASCs are highly clonally related in terms of Ab repertoires, despite distinct trajectories of phenotypic development. In addition, we characterized some phenotypic and transcriptional features of intestinal ASCs implicated in longevity, including HLA-DR^+/-CD95^- expression and the upregulation of Bcl-2, Blimp-1 and XBP-1. Finally, we generated metal isotope-labeled RV double-layered particles (DLPs) and validated their use in the identification of VP6-specific B cells by mass cytometry. We demonstrate broad VP6-binding reactivity across both intestinal and circulatory ASCs and other B-cell subsets in RV immune adults at steady-state, with phenotypes representative of the overall B-cell pool. This work provides novel resolution and insight into the phenotypic complexity of human intestinal B cells and to the mechanisms regulating humoral memory to an important mucosal pathogen at steady state.

Results

Combinatorial expression of mucosal trafficking markers in ASCs and other B-cell subsets in the intestine and blood at steady state

B cells isolated from proximal jejunum resections from adult gastric bypass patients and peripheral blood from the same patients (n=7) were used for immune phenotyping by mass cytometry. In addition, intestinal resections were obtained without simultaneous blood samples (n=3) or blood samples were obtained from a blood bank (n=10). Boolean gating was used to identify intestinal mature B cells as CD3/14/16/56^- CD19⁺ or CD27^hiCD38^hi.²³ Peripheral blood B cells were identified as CD19⁺. ASCs and non-ASC B-cell subsets (switched MBCs, CD27^- MBCs, IgM⁺ MBCs and naive B cells) were identified in the intestine and the blood of all donors (Figure 1a, Supplementary Figure S1–S3 online). Spanning-tree progression analysis of density-normalized events²⁴ was used to simultaneously measure the expression of mucosal trafficking markers on intestinal and circulating B cells. Nodes of phenotypically similar cell clusters corresponding to ASCs and non-ASC B-cell subsets were identified based on the expression of B-cell lineage markers in all seven donors analyzed (Figure 1b, Supplementary Figure S5) and were used to analyze quantitative and qualitative differences between subsets.^{15, 24, 25} In all donors analyzed, intestinal ASCs were CCR6^lo/-CCR7^lo/-CCR10⁺Intβ1⁺CXCR4⁺Alpha4^loIntβ7^lo/−; in contrast, circulating ASCs were CCR6^lo/-CCR7^+/-CCR10^+/-Intβ1⁺CXCR4⁺Alpha4^hiIntβ7⁺ (Figure 1c, Supplementary Figure S5). With the exception of naive B cells, all intestinal B cells expressed at least threefold higher levels of CXCR4 than circulating B cells (ASC, P=0.002; switched MBC, P<0.0001; IgM⁺ MBC, P=0.0004). Circulating ASCs expressed higher levels of Alpha4 and Intβ7 than intestinal ASCs (P<0.05). Analysis of the simultaneous expression of six mucosal trafficking markers in individual nodes of all the seven donors revealed a combinatorial diversity of phenotypes (Figure 2).

Intestinal ASCs exhibit phenotypic and transcriptional characteristics of long-lived plasma cells

Some of the phenotypic and transcriptional characteristics of intestinal ASCs (CD20^lo/- CD27^hi CD38^hi), implicated in longevity, were determined. Intestinal and circulating ASCs expressed lower levels of surface immunoglobulin A (IgA) and CD40 than switched MBCs, whereas intestinal ASCs expressed higher levels of CCR10 and CD138 compared with circulating ASCs (Figure 3a). In contrast to circulating ASCs that are HLA-DR^+/-CD95⁺, intestinal ASC are HLA-DR^-CD95^-, suggesting non-responsiveness to apoptosis via CD95 ligation (Figure 3a,b and c). Among the circulating ASCs that were HLA-DR^-, some expressed CD138 and all were CD95⁺ (Figure 3b). Based on quantitative reverse transcription-PCR analysis of sorted intestinal ASCs and MBCs (Supplementary Figure S4, Supplementary Table S2), the mRNA encoding antiapoptotic transcription factor Bcl-2 was expressed at higher levels in intestinal ASCs than in intestinal MBCs (P=0.048), as were Blimp-1 (P=0.038) and XBP-1 (P=0.009), upregulated during plasma cell differentiation²⁶ (Figure 3d, Supplementary Table S2). Bcl-6, required for germinal center formation, was expressed by intestinal MBCs but not by intestinal ASCs (P=0.013). IgA secretion was detected from sorted intestinal ASCs at steady-state (median frequency (range), 2.92 × 10⁵ per 10⁶ B cells (1.98 × 10⁵–4.58 × 10⁵)) and for up to 5 days in vitro in the presence of CpG-2006 and IL-2 (9.90 × 10⁴ per 10⁶ B cells (1.79 × 10⁴–1.80 × 10⁵)) (Supplementary Figure S3C, D, E, Supplementary Figure S4, Supplementary Table S2). Based on these measured parameters, these data suggest that intestinal ASCs share some phenotypic and transcriptional attributes with quiescent, terminally differentiated, long-lived bone marrow plasma cells²⁷ but are unlike pro-apoptotic plasmablasts in circulation or tonsil-derived plasma cells.²⁸ Analysis of additional transcriptional and functional features of intestinal and bone marrow ASCs in the same individuals will be required to further explore these findings.

Dimensionality reduction by PCA reveals phenotypic relationships between B-cell subsets in the intestine and blood

PCA was used to visualize the high-dimensional mass cytometry data sets.^{17, 18, 29} PCA defines components that cumulatively account for the variation contained within the entire data set, with the first three components in this analysis accounting for most of the total variation. PCA allows the patterns of expression of all 34 markers to be summarized for each cell, which can then be viewed on a two-dimensional or three dimensional (3D) plot, thereby allowing different cell populations to be viewed in relation to one another.^{18, 21, 29}

As the phenotypes of ASCs and non-ASCs were so different, PCA was more informative when they were analyzed separately (Figure 4a and b, Supplementary Figure S6A, B). Visualization of the first two principal components of ASCs (Figure 4b, Supplementary Figure S6B) and non-ASCs (Figure 4a, Supplementary Figure S6A) provided an overview of the phenotypic complexity of intestinal and circulating B cells. The general arrangement of clusters was conserved across the seven donors analyzed (Figure 4a and b). Non-ASC subsets were identified by manual gating (Supplementary Figure S3A), overlaid on two-dimensional plots, and used to identify the composition of the clusters (Figure 4a, Supplementary Figure S6A) as previously described.^{19, 20, 21} In the blood, IgM⁺ MBCs and naive B cells were phenotypically related and distinct from CD27^- MBCs and switched MBCs. CD27^- and switched MBCs were phenotypically more related to each other than to the IgM⁺ MBCs and naive B cells. This trend was also observed in the intestinal data; however, in some donors IgM⁺ MBCs also overlapped with switched MBCs, suggesting greater complexity of IgM⁺ MBCs in the intestinal milieu compared with the blood. ASCs, switched MBCs, and CD27^- MBCs were gated based on isotype expression and IgA⁺, IgM⁺, and IgG⁺ cells within each subset were identified (Figure 4c and d). IgG⁺ ASCs were phenotypically more distinct from IgA⁺ ASCs than were IgM⁺ ASCs. The amount of variance described by each principle component was quantified to estimate the cumulative effectiveness of each analysis. The first two principal components cumulatively accounted for 45% (27–50%) of the variation in the entire data set (Figure 4e and f, Supplementary Figure S6E). The addition of the third principal component increased the cumulative variation coverage to 50% (35–56%). Any single additional principal component, beyond the first three components, did not contribute to significantly more variation and thus were not included in analyses. Loading values, or weighting coefficients, provided insight on the contribution of each marker to the first two principal components (Figure 4g and h, Supplementary Figure S6F, G). This same analysis was run on each donor independently; the general features of the analysis were preserved on independent repeats among different donors analyzed (Supplementary Figure S6A, B).

Visualization of the first three principal components revealed an ordered arrangement of non-ASC B-cell subsets that recapitulated the trajectory of B-cell differentiation. Naive B cells and IgM⁺ MBCs were phenotypically closely related. CD27^- MBCs were observed as a transitional population between IgM⁺ MBCs and switched MBCs; this separation was more evident in the blood than in the intestine. Switched MBCs occupied a phenotypic niche distinct from the other non-ASC subsets (Figure 5a, Supplementary Movie S1A, B). A multitude of unique, transitional cell states were observed between the major B-cell subsets in the intestine and the blood.

3D PCA was used to directly compare the phenotypic relationships between intestinal and circulating ASCs and non-ASC B cells. ASCs in the intestine and blood were phenotypically distinct (Figure 5b). Non-ASCs in the intestine and blood partially clustered together but also exhibited some distinct, compartment-specific clusters (Figure 5c). To determine which specific non-ASC subsets were phenotypically related in the intestine and blood, each B-cell subset in the blood and intestine was plotted on an individual 3D plot. This analysis revealed that naive B cells are closely related in the intestine and the blood. With progressive B-cell differentiation, phenotypic relatedness decreased among non-ASC subsets in the intestine compared with the blood. Switched MBCs and ASCs occupied almost entirely distinct phenotypic niches in the two compartments (Figure 5d).

Total intestinal and circulating ASCs are clonally related as revealed by Ab repertoire lineage analysis

Having compared the phenotypes of intestinal and circulating B cells we then directly compared intestinal and circulating Ab lineages using a sequencing approach that incorporates unique barcode labels on the same Ab heavy chain (IGH) RNA molecule.³⁰ These labels permit multiple sequencing reads derived from the same RNA molecule after extensive amplification to be combined so as to reduce the sequencing error rate and enable accurate quantification of RNA and isotype. IGH lineages were defined as sequences with identical V and J segment usage and >90% identity in junction regions, thus originating from the same B-cell clone.

Total circulating ASCs and naive B cells were sorted to >96% purity from peripheral blood monuclear cells obtained from two donors and IGH lineages shared between sorted ASCs or naive B cells and the unsorted peripheral blood monuclear cells facilitated identification of subset-specific IGH lineages among the total peripheral blood monuclear cells based on IGH transcript abundance, mutation, and isotype (Figure 6a). Total ASCs expressed highly abundant, mutated class-switched IGH lineages while total naive B cells expressed low-abundance, non-mutated IgM lineages. These trends were consistent between the two subjects analyzed and were used to identify total ASC and naive B-cell lineages among total B cells in the intestine and the blood of the same donor (Figure 6b). The peripheral blood Ab repertoire was dominated by IgA and naive IgM lineages (Figure 6c). IgA lineages were highly abundant and therefore ASC-derived. Non-mutated IgM lineages were low in abundance and therefore derived from naive B cells (Figure 6d and e). The intestinal Ab repertoire was dominated by IgA lineages (Figure 6c), but was largely devoid of naive, low-abundance IgM lineages (Figure 6d and e), consistent with mass cytometry data (Supplementary Figure S3B) and previous studies.^{2, 31}

To determine the direct clonal relationships between total intestinal and circulating B cells, we compared the percentage of shared Ab lineages between the two compartments. Of note, there were 1053 highly abundant IgA lineages in circulating B cells; of these, 385 were also detected in intestinal B cells. The 36.5% of shared sequences was higher than the extent of shared lineages between peripheral blood samples obtained at the same time from the same individual (∼25%),³⁰ suggesting that the intestinal and circulating IgA⁺ ASCs are highly related (Figure 6b). Lineages were also shared among IgG⁺ (25.5%) and IgM⁺ (23.2%) ASCs in the intestine and the blood. Among non-ASCs, lineages were shared in IgA repertoires (11.6%) and few lineages were shared among IgG (4.1%) or IgM (1.5%) repertoires.

Major intestinal and circulating B-cell subsets demonstrate VP6 reactivity at steady state

The mass cytometry platform was then applied to the identification RV VP6-specific B cells. Rhesus RV DLPs, which possess an inner capsid composed of VP6 and VP2 but lack an outer capsid containing VP4 and VP7, were labeled with erbium 166 (Er166) and used to stain B cells expressing VP6-specific Ig. As metal-labeled antigens have not been used to identify antigen-specific B cells previously, the specificity and sensitivity of this approach was verified with B-cell receptor staining and blocking experiments in mice and humans. It is virtually impossible to obtain a RV-naive adult that could be used to verify DLP-specific B-cell staining. Therefore, we first demonstrated that DLP-Er166-stained VP6-specific but not VP4-specific murine hybridoma cells. Blocking with unlabeled DLPs reduced DLP-Er166-specific staining on VP6-specific hybridomas (Figure 7a). Next we stained human peripheral blood B cells with DLP-Er166 in the presence and absence of unlabeled DLPs. Unstained control samples were used to identify VP6-specific B cells. Blocking reduced DLP-Er166 staining on total B cells by 90% (0.45–0.05% of CD19⁺ B cells) (Figure 7b). In peripheral blood samples, DLP-Er166-binding cells were present among switched MBCs, IgM⁺ MBCs, CD27^- MBCs, and naive B cells (Figure 7c, Supplementary Figure S7A), consistent with previous flow cytometry studies using VLP-VP2-GFP/VP6.^{8, 9, 10} Lastly, blocking with unlabeled DLPs reduced DLP-Er166 binding to B cells in all B-cell subsets by greater than equal to eightfold (switched MBCs 0.28–0.04%; IgM⁺ MBCs 0.55–0.04%; CD27^- MBCs 0.53–0.07%; naive B cells 0.40–0.05%) (P<0.0001)(Figure 7c, Supplementary Figure S7A). The frequencies of VP6-specific B cells among total B cells (Supplementary Figure S7B) and among switched MBCs, IgM⁺ MBCs, CD27^- MBCs and naive B cells (Supplementary Figure S7C) were comparable using the mass cytometry and flow cytometry platforms. In parallel work in our laboratory, we have labeled DLPs with Cy5 and used this conjugate to single-cell sort intestinal IgA⁺ ASCs into RNA lysis buffer for reverse transcription-PCR amplification of Ig variable genes and mAb expression (Supplementary Figure S7D, E, F). Seven of the nine expressed mAbs bound DLPs, as determined by Elisa, further demonstrating specificity of the VP6-specific B-cell staining. Analysis of V gene segment usage among VP6-specific intestinal IgA⁺ ASCs revealed an over representation of the VH4 gene family, consistent with previous work by Di Niro et al.¹⁴

VP6-specific intestinal and circulating B cells from the same healthy, adult donors were characterized by mass cytometry. VP6-specific intestinal B cells were detected among ASCs, switched MBCs, IgM⁺ MBCs, CD27^- MBCs, and naive B cells (Figure 7d,e and f, Supplementary Figure S7B). This reactivity among diverse subsets was also observed in circulating B cells from the same donors: DLP-Er166 binding reactivity was observed to switched MBCs, IgM⁺ MBCs, CD27^- MBCs and naive B cells, consistent with previous studies.^{8, 9, 10} VP6-specific ASCs were present at a median frequency of 0.5% (0–0.1%) of total ASCs in the intestine and were not detected in the blood in the seven donors analyzed. The median frequency of VP6-specific switched MBCs was higher in the intestine (2.1% (0.3–3.0%)) compared to the blood (0.3% (0–2.8%); (P=0.007); (Figure 7e). In contrast, the median frequencies of VP6-binding IgM⁺ MBCs, CD27^- MBCs, and naive B cells in the intestine and blood were not significantly different (P=0.09, P=0.29, P=0.39). The phenotypes of VP6-specific B cells, compared with VP6-non-specific B cells, did not differ in ASCs or other B-cell subsets in the intestine or in the blood, as determined by clustered heatmap analysis of 34 cell surface markers (Figure 7g).

Discussion

In this study we provide a high-dimensional view of the phenotypic heterogeneity of human intestinal B cells, and use PCA to visualize the phenotypic relationships between ASCs and other B-cell subsets in the intestine compared to the blood. Human intestinal B cells have been extensively characterized by flow cytometry and immunohistochemistry.^{31, 32, 33, 34} As the mass cytometry platform facilitates the analysis of many more parameters per individual cell while circumventing problems of spectral overlap, we extend these fluorescence-based analyses to reveal phenotypic complexity at a systems level and a heterogeneity far greater than previously appreciated. These initial data lay the foundation for future studies to determine the microanatomical derivation of ASCs and non-ASC subsets within the intestinal mucosa as well as their functional contribution.

One of the major advances of this work is the validation of the mass cytometry platform for the identification of antigen-specific B cells, as demonstrated in the analysis of RV VP6-specific B cells. Virtually all adults have been infected with RV on several occasions and have established humoral memory that generally protects from repeat disease. Thus current RV vaccines do not generally induce detectable immune responses in adults as all adults are immune to these live viral vaccines owing to prior exposure, in many cases, multiple exposures. As RV is a ubiquitous pathogen, persistent exposure and subclinical infection likely result in the re-stimulation of RV-reactive MBCs to ASCs in the intestinal mucosa. Murine studies have also demonstrated persistence of RV-specific ASCs in the mouse intestine after a single RV infection.³⁵ We report the breadth of B-cell subsets with reactivity to VP6 that constitute part of this memory response in RV-infection experienced adults and frequencies of VP6-specific ASCs and switched MBCs that were significantly higher in the intestine (ASCs 0.5 vs 0%; switched MBCs 2.1 vs 0.3%), the primary site of RV infection, than in circulation. Although VP6-specific Abs will not block RV entry into epithelial cells, they may restrict viral replication.^{5, 6} This steady-state Ab response to a ubiquitous pathogen may represent a mechanism through which humoral memory to RV is maintained. It should be noted that most IgA⁺ and IgM⁺ ASCs maintain surface Ig expression. However, identification of IgG⁺ ASCs based on surface Ig expression is limited to a subset of cells that express surface Ig, as many IgG⁺ ASCs have downregulated expression of cell surface B-cell receptor.^{23, 36}

The functions of ASCs, switched MBCs, and IgM⁺ MBCs in mediating humoral immunity to RV have been studied;¹³ however, the roles of CD27^- MBCs and naive B cells remain unclear. Naive B cells specific to VP6 have been reported in circulation in healthy adults at steady state^{8, 9} and could represent an innate repertoire comprised of natural Abs owing to optimal VP6-interacting germline-encoded surface Ig, or may result from an early T-independent B-cell response to RV. Innate-like intestinal B cells capable of iNos and TNF-α secretion have been described in the mouse lamina propria.³⁷ Ab repertoire studies showed that usage of VH1-69 was higher in the VP6-specific naive B-cell repertoire compared with total naive B cells in circulation.¹⁰ This gene segment encodes a hydrophobic CDR-H2 and has been implicated in early viral responses and polyspecificity.³⁸

The observation that multiple B-cell subsets have reactivity to VP6 at steady state contrasts the B-cell response to tetanus toxoid³⁹ or to VP7,⁹ for which reactivity is present solely among switched MBCs. The broad VP6-binding reactivity may represent a regulated mucosal response to a non-neutralizing epitope present on a ubiquitous pathogen. During RV replication and cell lysis, a large amount of extracellular VP6 is exposed to the immune system. Regulating the class-switched, high-affinity response toward neutralizing epitopes present on VP4 and VP7, while directing some class-switched MBCs and but predominantly low affinity, IgM responses to VP6,^{13, 40} may be advantageous as IgG and IgA responses have been associated with intestinal inflammation.^{41, 42} Further studies are required to assess the functional contribution of VP6-specific B cells in mediating immunity to RV. As metal labeling of antigens is applicable to a range of proteins, these tools may aid in the improved understanding of the complexity of B cells induced by a variety of infections and vaccinations.¹⁶

Little is known regarding the migration and lifespan of intestinal ASCs. Several observations have suggested that mucosal plasma cells are short-lived;⁴³ however, plasma cells from small intestine biopsies secrete IgA in vitro for up to 4 weeks⁴⁴ and colonic and bone marrow plasma cells that express Bcl-2 are CD95^+/lo and non-responsive to CD95 ligation in vitro.³⁴ In contrast, circulating and tonsillar ASCs are CD95^hi and can be driven to apoptosis in vitro via CD95 ligation.²⁸ Here we demonstrated that, based on some phenotypic and transcriptional parameters, intestinal ASCs were similar to quiescent, terminally differentiated, long-lived bone marrow plasma cells (HLA-DR^-CD95^lo/-CD40^-CD180^-CD138^+/− phenotype and higher expression levels of Bcl-2, Blimp-1, IRF-4 and XBP-1 compared with intestinal MBCs). However, direct comparisons between intestinal and bone marrow ASCs in the same individuals, and measurement of the expression of Ki67, BCMA, and Mcl1 will be required to fully explore these initial findings. We detected persistent IgA secretion by intestinal ASCs for up to 5 days in vitro in the presence of CpG-2006 and IL-2. The downregulation of CD95 was unique to intestinal ASCs, as all circulating ASCs, including the few that were HLA^-DR^-CD138^+/−, expressed high levels of CD95. CXCL12, the ligand for CXCR4, is important for the survival of bone marrow plasma cells⁴⁵ and for plasmablast homing to the bone marrow.⁴⁶ We observed higher expression of CXCR4 on intestinal ASCs compared with circulating ASCs; however, whether or not mucosal tissue provides survival niches for long-lived plasma cells remains unclear.⁴⁷ Analysis of proliferation markers, such as Ki67, and the expression of Mcl1,⁴⁸ associated with antiapoptotic pathways in plasma cells, will be required to extend these initial observations. Interestingly, we detected expression of Bcl-6 in intestinal MBCs. Bcl-6 expression is associated with germinal center B cells;⁴⁹ however, germinal center B cells were excluded from this analysis as we identified intestinal MBCs based on CD20⁺ CD27⁺ CD38^int/- phenotype and sorted these cells to >99% purity. Furthermore, we verified that the intestinal MBCs secreted IgA following a 5 day in vitro stimulation with CpG-2006 and IL-2. Of note, the expression of CCR9 has been implicated as an important trafficking marker to the human small intestine; however, due to lack of suitable staining with available Ab clones we were unable to stain for this marker. However, circulating ASCs expressed the gut-trafficking markers, Alpha4 and Intβ7, that were downregulated in intestinal ASCs. Intestinal ASCs expressed Intβ1 and CXCR4, factors implicated in ASC trafficking to the upper aerodigestive epithelium and bone marrow respectively,⁵⁰ suggesting a promiscuous homing capacity for these cells.

Clonal relationships between IgG⁺ B cells in the colon and blood,⁵¹ and between colonic B cells and associated lymph nodes⁵² from ulcerative colitis patients have been previously described. This is the first direct report of the clonal relationships between total small intestinal and circulating IgA⁺, IgG⁺, and IgM⁺ ASCs, and other B cells in donors without gastrointestinal disease. We observed strong clonal relationships between total intestinal and circulating IgA⁺ ASCs. The majority of circulating IgA⁺ ASCs are derived from mucosal immune reactions that occur in the intestine and in lymphoid tissues of the gut and the airway.⁵³ Most circulating IgA⁺ ASCs detected at steady state are likely homing to the gut, where they proliferate and generate local clones.⁵⁴ Although our Ab repertoire analysis did not discriminate between B cells entering the intestinal mucosa versus tissue-resident B cells, these findings do support trafficking between the gut and peripheral blood. Although circulating and intestinal ASCs were highly clonally related, intestinal ASCs displayed distinct phenotypes that may reflect imprinting in the intestinal microenvironment.

Dimensionality reduction by PCA revealed novel phenotypic relationships between B-cell subsets.^{17, 18, 29} In particular we demonstrated that IgM⁺ MBCs were phenotypically most related to naive B cells. The origin of IgM⁺ MBCs in humans is controversial as these cells express CD27, can rapidly differentiate to ASCs, and exhibit somatic hypermutation in VH genes yet have been detected in the absence of antigen exposure and in patients with genetic defects in CD40-CD40L.⁵⁵ Human IgM⁺ MBCs have been referred to as natural MBCs owing to their ability to be generated in vitro by CpG stimulation of transitional B cells.⁵⁶ In our analysis, intestinal IgM⁺ MBCs were phenotypically related to both naive B cells and switched MBCs but had a distinct phenotype from any other B-cell subset. It is likely that individual subpopulations of IgM⁺ MBCs may differ in their origin and requirement for T-cell stimulus.

The measurement of multiple markers per individual cell facilitated visualization of the phenotypic relationships between major B-cell subsets as has previously been performed for T and B cells.^{19, 20, 21, 22} In 3D PCA we determined that CD27^- MBCs occupied a transitional phenotypic niche between IgM⁺ MBCs and switched MBCs. CD27^- MBCs produce Abs against encountered antigens such as tetanus toxoid and influenza but not against pneumococcal polysaccharides.⁵⁷ CD27^- IgA⁺ MBCs in circulation have limited proliferation potential and are present in CD40L-deficient patients, reflecting a GC-independent origin. In contrast CD27^-IgG⁺ MBCs are likely derived from GC reactions.⁵⁸ In our analysis most intestinal CD27^- MBCs were IgA⁺, a subset associated with innate effector functions⁵⁸ and T-independent responses. Within the ASC, switched MBC, and CD27^- MBC subsets, distinct phenotypic clusters were identified in PCA based on isotype expression. IgG⁺ ASCs were phenotypically more distinct from IgA⁺ ASCs and IgM⁺ ASCs. As intestinal and circulating IgA⁺ ASC were clonally related, the distinct phenotypes of IgG⁺ ASC may reflect distinct regulation of IgG⁺ plasma cell differentiation.³⁶ Further studies will be required to investigate the functional implications of these observations.

In conclusion, we used mass cytometry to provide a high-dimensional view of the phenotypic heterogeneity of total and VP6-specific intestinal and circulating B cells. We establish the use of metal-tagged proteins for high-dimensional analysis of antigen-specific B cells. This work broadens our understanding of the regulation of humoral memory at mucosal surfaces and lays the foundation for understanding perturbations induced by disease. These data may also aid in the design of improved next generation mucosal vaccines.

Methods

Human subjects. Proximal jejunum tissue resections were obtained from adults undergoing bariatric surgery at the Stanford Hospital for weight reduction. Informed consent was obtained to collect peripheral blood (40 ml) intra-operatively. Leukocyte reduction system chambers containing peripheral blood monuclear cells from aphaeresis donors were obtained from the Stanford Blood Center. This study was performed in accordance with Stanford University Institutional Review Board protocols.

Isolation of B cells from jejunum tissue and peripheral blood. Jejujum tissue resections were processed within 1–2 h of surgery. Viable mononuclear cells representative of the lymphoid population present in the gastrointestinal mucosa were isolated as described⁵⁹ with modification. Tissue fragments were digested for 1 h at 37 °C with Liberase TL (Roche, Basel, Switzerland) (0.26 Wünsch Units/ml). Intestinal and circulating B cells were enriched using EasySep Human B cell Enrichment Kit without CD43 Depletion and RosetteSep Human B cell Enrichment Cocktail (Stemcell Technologies, Vancouver, BC, Canada), respectively, according to manufacturer’s instructions. Isolated B cells were incubated at 37 °C in 5% CO₂ for 2 h prior to staining. Cell surface markers that were expressed at comparable levels with and without liberase treatment were included in the analysis.

Antibody labeling. Unconjugated antibodies (carrier protein-free) listed in Supplementary Table S1 were labeled with heavy metal-loaded maleimide-coupled MaxPar chelating polymers (DVS Sciences, Sunnyvale, CA) according to manufacturer’s instructions. Independent comparisons of DN3 and X8 polymers by the manufacturer show minimal differences and thus DN3 polymers were used to be consistent with previous experiments. Metal-labeled Abs were titrated and tested to ensure that staining patterns were consistent with those previously reported by flow cytometry.¹⁹

Rhesus RV DLP preparation and labeling. Rhesus RV (RRV) was grown in MA104 cells in the presence of trypsin as described.⁶⁰ RRV triple-layered particles were purified from MA104 cell lysates by genetron extraction, centrifugation through a sucrose cushion, and cesium chloride density gradient centrifugation as described.^{61, 62} DLPs were generated by treating triple-layered particles with 20 nM EDTA to remove VP4 and VP7. Purified DLPs were dialyzed to remove residual cesium chloride. DLPs (100 μg) were labeled with Er166-loaded maleimide-coupled DN3 MaxPar chelating polymer (DVS Sciences) according to manufacturer’s instructions.

Mass cytometry staining and data acquisition. B cells were stained for mass cytometry analysis as described^{19, 20} with the Abs listed in Supplementary Table S1. Data were acquired on the CyTOF 2 instrument (DVS Sciences) with noise-reduction mode turned off. For VP6-specific B-cell staining, 2 μg of DLP-Er166 was used to stain 2–3 million B cells for 45 min on ice prior to staining with the metal-tagged antibody cocktail. The concentration of DLP-Er166 used for staining was determined in titration experiments on VP6- and VP4-specific hybridomas (not shown).

Mass cytometry data analysis. Mass cytometry data were analyzed using FlowJo (version 9.4, Tree Star, Ashland, OR) using FCS files generated by cell identifying software.¹⁹ For Spanning-tree progression analysis of density-normalized events analysis, clustering was performed using Cytobank (DVS Sciences)²⁴ based on the expression of B-cell lineage markers among all donors analyzed. For PCA, total circulating and intestinal B cells were split into ASC versus non-ASC groups, exported as FCS files, and imported into R using the flowCore R package.⁶³ Each experiment, consisting of pairs of blood and intestine-derived B cells from the same patient, was analyzed separately. ASC and non-ASC groups were also analyzed separately. To equally weight the blood and intestine-derived cells, an equal number of cells were randomly selected from each sample and used for PCA using the prcomp R function. Scores for each component calculated for each cell were appended to the FCS files and used for subsequent analysis in FlowJo.¹⁹ For 3D plots, a protein data bank formatted file was exported and visualized using PyMol software.⁶⁴

Flow cytometry. VP6-specific B cell staining was performed with VLP VP2-eGFP/VP6 (2 μg per test) prior to staining B cells with a fluorescently tagged Ab panel as described (anti-CD3-PE Cy7 (clone: SKY, Becton Dickinson, San Jose, CA), anti-CD14 PE Cy7 (clone: M5E2, BD), anti-CD16-PE Cy7 (clone: 3G8, BD), anti-CD56-PE Cy7 (clone: B159, BD), anti-CD19 (clone: Sj25C1, BD), anti-CD20-APC H7 (clone: 2H7, BD), anti-CD27-PE (clone: MT271, BD), anti-CD38 (clone: HIT2, BD), anti-IgA (clone: IS11-8E10, Miltenyi Biotec, Auburn, CA), and anti-IgG (clone: G18-145, BD)).^{8, 13} VLPs VP2-eGFP/VP6 were generated as described.⁶⁵ The concentration of VLPs required per staining reaction was determined in titration experiments on VP6- and VP4-specific hybridomas. Data were acquired on the BD FACS Aria III or LSR II with DIVA software version 8.0 (Becton Dickinson). At least 100, 000 events were acquired per sample.

Elispot. The frequencies of total IgA⁺ ASCs were determined by Elispot as described.¹³ The frequency of intestinal IgA⁺ ASCs was determined prior to and following sorting of ASCs and MBCs with and without 1000 U ml^-1 IL-2 (R&D Systems, Minneapolis, MN) and 2.5 μg ml^-1 CpG-2006 (Invivogen, San Diego, CA) for 5 days at 37 °C in 5% CO₂. Cells were harvested after 5 days of stimulation, washed three times in complete media, plated and analyzed as described above.

Quantitative reverse transcription-PCR. Total RNA was isolated from enriched intestinal B cells and sorted intestinal ASCs and MBCs using RNeasy Micro kit (Qiagen, Venlo, the Netherlands). Reverse transcription was performed with Oligo dT using the Affinity Script QPCR cDNA Synthesis kit (Agilent, Santa Clara, CA). Real-time quantitative PCR for human B-2 microglobulin, Bcl-2, Bcl-6, Blimp-1, IRF-4, and XBP-1 transcripts was performed using Taqman gene expression assays (Applied Biosystems, Foster City, CA). Three independent PCRs were performed in triplicate, and results were analyzed using the comparative method with normalization of the expression of each transcript to 1 in the pre-sorted enriched B-cell fraction. Quantitative PCRs with threshold cycle (Ct) >35 were not considered significant.

Immune Repertoire Sequencing. Total RNA (500 ng) was used for library preparation. Reverse transcription was performed using primers for isotypes containing eight random nucleotide and partial Illumina adapters containing barcodes. Second-strand synthesis was performed using primers containing eight random nucleotide and partial Illumina adapter sequences covering all V segments with a maximum of one mismatch. Complementary DNA was purified and amplified and sequencing libraries were generated by purifying the PCR product. Data were analyzed as described with IGH lineages defined by 90% nucleotide identity between CDR3s.³⁰

Statistical analysis. Statistical analyses were performed using GraphPad Prism (version 6.0b). Correlations were performed using Pearson’s or Spearman’s tests. The unpaired t-test was used to compare differences between groups. P-values <0.05 were considered significant.

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Acknowledgements

The authors would like to thank M. Leipold for mass cytometry instrument assistance, N. Sigal for help with reagents, M. Metruccio for advice on quantitative reverse transcription-PCR analysis, and H. Mei for critical feedback. This work was supported by NIH AI021362, U19 AI090019 (US/India Bilateral Collaborative subaward) and NIH AI057229. N.N. is supported by the Lucile Packard Foundation for Children’s Health, NIH-NCATS-CTSA UL1 TR001085 and Child Health Research Institute of Stanford.

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Department of Medicine, Stanford University, Stanford, California, USA
N Nair, X S He & H B Greenberg
Department of Microbiology and Immunology, Stanford University, Stanford, California, USA
N Nair, E W Newell, M M Davis, X S He & H B Greenberg
Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), Singapore, Singapore
E W Newell
Department of Bioengineering, Stanford University, Stanford, California, USA
C Vollmers & S R Quake
Department of Surgery, Stanford University School of Medicine, Stanford, California, USA
J M Morton
The Howard Hughes Medical Institute, Stanford, California, USA
M M Davis
Institute for Immunity, Transplantation and Infection, Stanford University, Stanford, California, USA
M M Davis

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Nair, N., Newell, E., Vollmers, C. et al. High-dimensional immune profiling of total and rotavirus VP6-specific intestinal and circulating B cells by mass cytometry. Mucosal Immunol 9, 68–82 (2016). https://doi.org/10.1038/mi.2015.36

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Received: 10 September 2014
Accepted: 07 March 2015
Published: 22 April 2015
Issue Date: January 2016
DOI: https://doi.org/10.1038/mi.2015.36

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