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MicroRNA-155 promotes G-CSF-induced mobilization of murine hematopoietic stem and progenitor cells via propagation of CXCL12 signaling

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Acknowledgements

We thank Dr Ziv Porat for technical assistance with ImageStream analysis and Dr Ron Rotkopf for assistance with statistical data analysis. TL was supported by the Ministry of Science, Technology and Space, Israel and the DKFZ, Germany, grants from the Israel Science Foundation (851/13), the Ernest and Bonnie Beutler Research Program of Excellence in Genomic Medicine, and EU FP7-HEALTH-2010 (CELL-PID #261387). FK was supported from the Deutsche Krebshilfe (109420, Max-Eder program); a fellowship 2010/04 by the European Hematology Association; and by the Deutsche Forschungsgemeinschaft (DFG) (SFB 1074, project A5). AR was supported by the DFG (SFB 1074, project A5) as well as the gender equality program by the DFG (SFB 1074, project Z2). WGK was supported by NIH grants R01 HL072523 and R01 HL107127, the Paige Arnold Butterfly Run, and is an Empire Scholar of the State University of New York.

Author contributions

TI designed and performed the experiments, analyzed the data and wrote the manuscript; AK performed the experiments and edited the revised version of manuscript; SG-C, OK, KG and EK-M performed the experiments; and AR and ES designed and performed the miR-155-transduced HSPC proliferation, colony formation and Transwell assay. CL, HG and FK designed and performed miR-155 qRT-PCR-related experiment, analyzed the data and helped writing the manuscript; RB, CMR, JDC and WGK synthesized 3AC and helped in designing 3AC-related experiments; EH helped and guided in the design of experiments; and TL guided and designed the research and wrote the manuscript.

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Correspondence to F Kuchenbauer or T Lapidot.

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Competing interests

WGK has an equity position in a private venture that may license and commercialize SHIP-1 inhibitor technology for mobilization and harvest of BM stem cells. The remaining authors declare no conflict of interest.

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Supplementary Information accompanies this paper on the Leukemia website

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Itkin, T., Kumari, A., Schneider, E. et al. MicroRNA-155 promotes G-CSF-induced mobilization of murine hematopoietic stem and progenitor cells via propagation of CXCL12 signaling. Leukemia 31, 1247–1250 (2017). https://doi.org/10.1038/leu.2017.50

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