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Mtss1(CSC156) mutant mice fail to display efficient Mtss1 protein depletion

Leukemia volume 31, pages 1017–1019 (2017)Cite this article

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The metastasis suppressor 1 (Mtss1) protein belongs to the inverse Bin–amphiphysin–Rvs (I-BAR) domain family, a subgroup of membrane binding and deforming proteins involved in the formation of plasma membrane protrusions such as filopodia and lamellipodia. Mtss1 function appears to be pleotropic with tumor-promoting and tumor-suppressing functions being described in diverse malignancies.1, 2 Our own group established a role for Mtss1 as a novel tumor suppressor in chronic myeloid leukemia and acute myeloid leukemia.3, 4

Aiming to study the role of Mtss1 in vivo, two mouse models targeting Mtss1 expression had previously been developed. In the first model, insertion of a neomycin cassette into the first Mtss1 exon resulted in the depletion of Mtss1 full-length protein, but preserved an I-BAR truncated isoform.5 In a second model, Mtss1 knockout was reported to be achieved by utilization of embryonic stem cells (ES, clone CSC156, BayGenomics) carrying an insertion of a gene-trapping vector (pGT0Lxf) into the third intron of the Mtss1 gene. Using this Mtss1CSC156 model, the authors describe complete Mtss1 depletion in homozygous mutant mice6 resulting in a predisposition for lymphoma development7 and increased hematopoietic stem cell homing.8 Intending to further investigate Mtss1 function in blood malignancies, we attempted to establish the latter mouse model in our laboratory.

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Acknowledgements

We thank Kristina Feldberg for excellent technical assistance.

Author contributions

DF and MS designed the research; DF and OH performed the research; DF, SK, THB and MS analyzed the data and edited the manuscript; DF and MS wrote the manuscript. All authors approved the final version of the manuscript.

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Authors and Affiliations

  1. Department of Hematology, Oncology, Hemostaseology, and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University, Aachen, Germany

    D Fahrenkamp, O Herrmann, S Koschmieder, T H Brümmendorf & M Schemionek

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  1. D Fahrenkamp
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  2. O Herrmann
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  3. S Koschmieder
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  4. T H Brümmendorf
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  5. M Schemionek
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Correspondence to M Schemionek.

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Fahrenkamp, D., Herrmann, O., Koschmieder, S. et al. Mtss1(CSC156) mutant mice fail to display efficient Mtss1 protein depletion. Leukemia 31, 1017–1019 (2017). https://doi.org/10.1038/leu.2017.19

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