Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter to the Editor
  • Published:

In-vivo detectable antibodies directed against the oncofetal antigen/immature laminin receptor can recognize and control myeloma cells—clinical implications

Leukemia volume 22, pages 2115–2118 (2008)Cite this article

All patient sera with significant anti-OFA/iLR antibody levels were included in an epitope-mapping assay to confirm epitope specificity. A set of 42 synthetic peptides (OFA1–42) with a length of 17 amino acids (aa) overlapping by five aa and representing the entire sequence of OFA/iLR protein were used in a IgG and IgM ELISA. With regard to the localization of functional domains of OFA/iLR, the distribution of specific IgG and IgM responses includes association with intracellular as well as extracellular located epitopes (data not shown).

To look on long-term persistence of humoral anti-OFA/iLR immune responses, we were able to detect significant and stable titers of anti-OFA/iLR-specific IgG antibodies in follow-up samples of MGUS patients (n=6, panel f). IgG subclass analyses showed a predominant IgG1 and IgG3 response suggestive for a Th1-driven immune response in the vast majority of patients with MGUS and to smaller extent in patients with AMM (panel g).

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

References

  1. Zhang L, Conejo-Garcia JR, Katsaros D, Gimotty PA, Massobrio M, Regnani G et al. Intratumoral T cells, recurrence, and survival in epithelian ovarian cancer. N Engl J Med 2003; 348: 203–223.

    Article  CAS  PubMed  Google Scholar 

  2. Dave SS, Wright G, Tan B, Rosenwald A, Gascoyne RD, Chan WC et al. Prediction of survival in follicular lymphoma based on molecular features of tumor-infiltrating immune cells. N Engl J Med 2004; 351: 2159–2169.

    Article  CAS  PubMed  Google Scholar 

  3. Dhodapkar MV, Krasovsky J, Osman K, Geller MD . Vigorous premalignancy-specific effector T cell response in the bone marrow of patients with monoclonal gammopathy. J Exp Med 2003; 198: 1753–1757.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Dhodapkar MV . Harnessing host immune responses to preneoplasia: promise and challenges. Cancer Immunol Immunother 2005; 54: 409–413.

    Article  PubMed  Google Scholar 

  5. Spisek R, Kukreja A, Chen LC, Matthews P, Mazumder A, Vesole D et al. Frequent and specific immunity to the embryonal stem cell-associated antigen SOX2 in patients with monoclonal gammopathy. J Exp Med 2007; 204: 831–840.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Girardi AJ, Reppucci P, Dierlam P, Rutala W, Coggin Jr JH . Prevention of simian virus 40 tumors by hamster fetal tissue: influence of parity status of donor females on immunogenicity of fetal tissue and on immune cell cytotoxicity. Proc Natl Acad Sci USA 1973; 70: 183–186.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Coggin Jr JH, Ambrose KR, Anderson NG . Phase specific surface autoantigens on membranes of fetus and tumors. Adv Exp Med Biol 1973; 29: 483–490.

    Article  CAS  PubMed  Google Scholar 

  8. Coggin Jr JH, Barsoum AL, Rohrer JW, Thurnher M, Zeis M . Contemporary definitions of tumor specific antigens, immunogens and markers as related to the adaptive responses of the cancer-bearing host. Anticancer Res 2005; 25: 2345–2355.

    CAS  PubMed  Google Scholar 

  9. Siegel S, Wagner A, Friedrichs B, Wendeler A, Wendel L, Kabelitz D et al. Identification of HLA-A*0201-presented T cell epitopes derived from the oncofetal antigen-immature laminin receptor protein in patients with hematological malignancies. J Immunol 2003; 176: 6935–6944.

    Article  Google Scholar 

  10. Siegel S, Wagner A, Kabelitz D, Marget M, Coggin J, Barsoum A et al. Induction of cytotoxic T-cell responses against the oncofetal antigen-immature laminin receptor for the treatment of hematologic malignancies. Lymphocytes against hematologic neoplasms by survivin RNA-transfected dendritic cells. Blood 2003; 102: 4416–4423.

    Article  CAS  PubMed  Google Scholar 

  11. Rohrer JW, Barsoum AL, Coggin Jr JH . Identification of oncofetal antigen/immature laminin receptor protein epitopes that activate BALB/c mouse OFA/iLRP-specific effector and regulatory T cell clones. J Immunol 2006; 176: 2844–2856.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by part of the DFG ZE 697/2-1 grant.

Author information

Author notes

  1. S Siegel and B Friedrichs: These authors contributed equally to this paper.

Authors and Affiliations

  1. Department of Hematology and Stem Cell Transplantation, Georg, Hamburg, Germany

    S Siegel, A-K Budde & M Zeis

  2. Department of Hematology, Oncology and Transfusion Medicine, Charité-Campus Benjamin Franklin, Berlin, Germany

    B Friedrichs

  3. Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile, USA

    A Barsoum & J Coggin Jr

  4. Institute of Hematopathology, Hamburg, Germany

    M Tiemann

  5. Institute of Immunology, University of Kiel, Kiel, Germany

    D Kabelitz

Authors
  1. S Siegel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B Friedrichs
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A-K Budde
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A Barsoum
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J Coggin Jr
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M Tiemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. D Kabelitz
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M Zeis
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M Zeis.

Additional information

The authors declare no financial conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Siegel, S., Friedrichs, B., Budde, AK. et al. In-vivo detectable antibodies directed against the oncofetal antigen/immature laminin receptor can recognize and control myeloma cells—clinical implications. Leukemia 22, 2115–2118 (2008). https://doi.org/10.1038/leu.2008.92

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/leu.2008.92

Search

Advanced search

Quick links