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Successful peripheral blood stem cell mobilization with granulocyte colony-stimulating factor in a patient with chronic myeloid leukemia achieving a complete cytogenetic remission with dasatinib after failing imatinib

Leukemia volume 22, pages 1618–1619 (2008)Cite this article

Dasatinib is an orally active inhibitor of both ABL and SRC kinases, shown in both phase I and phase II clinical trials to be active in patients with chronic myeloid leukemia (CML) resistant to imatinib mesylate in all phases of the disease.1, 2, 3, 4 Dasatinib induces complete cytogenetic responses (CCyR) in more than 40% of the patients treated after imatinib failure while in chronic phase (CP) irrespective of the presence of kinase domain (KD) mutations.4

A 30-year-old man presented elsewhere with CML-CP in 2002. He became intolerant of interferon-α and was commenced on imatinib mesylate at a dose of 400 mg daily. At 12 months he had failed to achieve a major CCyR (92% Philadelphia (Ph)-positive cells in the marrow) and imatinib was increased first to 600 mg and then to 800 mg per day. No response was seen after 4 months on the higher doses and the patient was referred to our center. Bone marrow cytogenetic analysis showed 100% Ph-positive metaphases with no additional abnormalities. BCR-ABL transcripts measured 47.8% by real-time quantitative (RQ)-PCR with ABL as control gene. Mutation analysis revealed an E453V (A1358T) KD mutation using direct sequencing; pyrosequencing confirmed that 75% of Ph-positive cells carried the mutation. This mutation has not been reported previously in patients treated with imatinib.

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References

  1. Talpaz M, Shah NP, Kantarjian H, Donato N, Nicoll J, Paquette R et al. Dasatinib in imatinib-resistant Philadelphia chromosome-positive leukemias. N Engl J Med 2006; 354: 2531–2541.

    Article  CAS  Google Scholar 

  2. Guilhot F, Apperley J, Kim DW, Bullorsky EO, Baccarani M, Roboz GJ et al. Dasatinib induces significant hematologic and cytogenetic responses in patients with imatinib-resistant or -intolerant chronic myeloid leukemia in accelerated phase. Blood 2007; 109: 4143–4150.

    Article  CAS  Google Scholar 

  3. Cortes J, Rousselot P, Kim DW, Ritchie E, Hamerschlak N, Coutre S et al. Dasatinib induces complete hematologic and cytogenetic responses in patients with imatinib-resistant or -intolerant chronic myeloid leukemia in blast crisis. Blood 2007; 109: 3207–3213.

    Article  CAS  Google Scholar 

  4. Hochhaus A, Kantarjian HM, Baccarani M, Lipton JH, Apperley JF, Druker BJ et al. Dasatinib induces notable hematologic and cytogenetic responses in chronic-phase chronic myeloid leukemia after failure of imatinib therapy. Blood 2007; 109: 2303–2309.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Haematology, Hammersmith Hospital, London, UK

    I Gabriel, A Chaidos, J Sorouri, E Dannie, J Davis, J Goldman, J Apperley & D Marin

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  1. I Gabriel
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  2. A Chaidos
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  3. J Sorouri
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  4. E Dannie
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  5. J Davis
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  6. J Goldman
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  7. J Apperley
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  8. D Marin
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Correspondence to D Marin.

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Gabriel, I., Chaidos, A., Sorouri, J. et al. Successful peripheral blood stem cell mobilization with granulocyte colony-stimulating factor in a patient with chronic myeloid leukemia achieving a complete cytogenetic remission with dasatinib after failing imatinib. Leukemia 22, 1618–1619 (2008). https://doi.org/10.1038/leu.2008.13

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