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Artificial reproduction of the Yangtze field vole: in vitro embryo development and fertilization with fresh and freezethawed sperm

Abstract

Several research groups are using the Yangtze field vole as a model for studying schistosome infection, but relatively little is known about the species's reproductive physiology. The authors examined the vole's in vivo and in vitro embryonic development as well as the efficacy of in vitro fertilization using either fresh or cryopreserved sperm to breed these rodents.

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References

  1. Li, S.K., Zhu, Z.L., Jin, B.R., Zhou, D.R. & Zhuo, S.J. None infectiousness of Schistosoma japonicum to the field mouse Microtus fortis. Acta Parasitol. Sinica 2(1), 103–106 (1965).

    Google Scholar 

  2. Zhu, G.Z., Wang, Y.H., Fu, G.Y. & Liu, R.S. Breeding the Microtus fortis in laboratory condition and the none infectiousness of Schistosoma japonicum. Shanghai Lab. Anim. Sci. 11(4), 193–198 (1991).

    Google Scholar 

  3. Jiang, S.F. et al. Preliminary study on natural antibodies and IgG subclasses against Schistosoma japonicum in Microtus fortis. Chin. J. Schisto. Control 13(1), 1–3 (2001).

    Google Scholar 

  4. Ross, A.G. et al. Schistosomiasis in the People's Republic of China: prospects and challenges for the 21st century. Clin. Microbiol. Rev. 14(2), 270–295 (2001).

    Article  Google Scholar 

  5. He, Y.K. et al. Immunological characteristics of natural resistance in Microtus fortis to infection with Schistosoma japonicum. Chin. Med. J. (Engl) 112(7), 649–654 (1999).

    Google Scholar 

  6. Fu, Z.Q. et al. Observation and analysis of Schistosoma japonicum specific antibodies in sera of Microfus fortis. Chin. J. Vet. Parasitol. 9(4), 6–9 (2001).

    Google Scholar 

  7. Liu, J.M. et al. Preliminary observation on the killing effect to shistosomula of Schistosoma Japonicum in vitro by ADCC of Microtus fortis. Acta Parisitol. Med. Entomol. Sin. 8(4), 212–219 (2001).

    Google Scholar 

  8. Shen, Z. M & Yu, J.H. Study on domesticating and breeding of Microtus fortis Bücher. Shanghai Lab Anim. Sci. 15(2), 88–90 (1995).

    Google Scholar 

  9. Nakagata, N. et al. Cryopreservation of spermatozoa of a transgenic mouse. Jikken Dobutsu. 41(4), 537–540 (1992).

    Google Scholar 

  10. Penfold, L.M. et al. A new method for cryopreservation of mouse spermatozoa. J. Reprod. Fertil. 99(1), 131–134 (1993).

    Article  Google Scholar 

  11. Songsasen, N., Betteridge, K.J. & Leibo, S.P. Birth of live mice resulting from oocytes fertilized in vitro with cryopreserved spermatozoa. Biol Reprod. 56(1), 142–152(1997).

    Google Scholar 

  12. Storey, B.T., Noiles, E.E. & Thompson, K.A. Comparison of glycerol, other polyols, trehalose, and raffinose to provide a defined cryoprotectant medium for mouse sperm cryopreservation. Cryobiology 37(1), 46–58 (1998).

    Article  Google Scholar 

  13. Tada, N. et al. Cryopreservation of mouse spermatozoa in the presence of raffinose and glycerol. J. Reprod. Fertil. 89(2), 511–516 (1990).

    Article  Google Scholar 

  14. Wakayama, T. et al. Development of early-stage embryos of the Japanese field vole, Microtus montebelli, in vivo and in vitro. J. Reprod. Fertil. 101(3), 663–666 (1994).

    Article  Google Scholar 

  15. Kaibara Shichiro, The Experiment Method of Mammalian Animal Developing Engineering 291 (Nanjing University, Nanjing, China, 1992).

    Google Scholar 

  16. Mystkowska, E.T. et al. Preimplantation development in vivo and in vitro in bank voles, Clethrionomys glareolus, treated with PMSG and hCG. J. Reprod. Fertil. 42(2), 287–292 (1975).

    Article  Google Scholar 

  17. Hoshi, M. & Toyoda, Y. Effect of EDTA on the preimplantation development of mouse embryos fertilized in vitro. Jpn. J. Zootech. Sci. 56, 931–937 (1985).

    CAS  Google Scholar 

  18. Schini, S.A. & Bavister, B.D. Development of golden hamster embryos through the two-cell block in chemically defined medium. J. Exp. Zool. 245(1), 111–115 (1988).

    Article  Google Scholar 

  19. Miyoshi, K., Funahashi, H., Okuda, K. & Niwa, K. Development of rat one-cell embryos in a chemically defined medium:effects of glucose, phosphate and osmolarity. J. Reprod. Fertil. 100(1), 21–26 (1994).

    Article  Google Scholar 

  20. Abramczuk, J., Solter, D. & Koprowski, H. The beneficial effect of EDTA on the development of mouse one-cell embryos in chemically defined medium. Dev. Biol. 61(2), 378–383 (1977).

    Article  Google Scholar 

  21. Brison, D.R. & Leese, H.J. Blastocoel cavity formation by preimplantation rat embryos in the presence of cyanide and other inhibitors of oxidative phosphorylation. J. Reprod. Fertil. 101(2), 305–309 (1994).

    Article  Google Scholar 

  22. Brown, J.J. & Whittingham, D.G. The role of pyruvate, lactate and glucose during preimplantation development of embryos from F1 hybrid mice in vitro. Development 112(1), 99–105 (1991).

    Google Scholar 

  23. Watson, P.F. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertil. Dev. 7(4), 871–891 (1995).

    Article  Google Scholar 

  24. Nishizono H. et al. Decrease of fertilizing ability of mouse spermatozoa after freezing and thawing is related to cellular injury. Biol. Reprod. 71(3), 973–978 (2004).

    Article  Google Scholar 

  25. Müller, K. et al. Stability of transbilayer phospholipids asymmetry in viable ram sperm cells after cryotreatment. J. Cell Sci. 112(Pt1), 11–20 (1999).

    Google Scholar 

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Correspondence to Li-Jun Liu.

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Liu, LJ., Xu, P., Gu, Jz. et al. Artificial reproduction of the Yangtze field vole: in vitro embryo development and fertilization with fresh and freezethawed sperm. Lab Anim 35, 37–41 (2006). https://doi.org/10.1038/laban0906-37

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