Abstract
By using a conventional two-hybrid technique with an Src homology 3 (SH3) domain of Nesh as the bait protein, a novel full-length cDNA was isolated and sequenced from a human placenta cDNA library. This cDNA consists of 3023 bp and has a predicted open reading frame that encodes 486 amino acids. It possesses an SH3 binding motif, a nuclear targeting sequence, and no catalytic domain. Overall, it has no similarity to known molecules involved in a signaling cascade. Polymerase Chair reaction-based mapping with both a monochromosomal hybrid panel and radiation hybrid cell panels localized the gene on human chromosome 3q12 near the marker D3S1271.
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Received: March 12, 2001 / Accepted: April 23, 2001
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Matsuda, S., Iriyama, C., Yokozaki, S. et al. Cloning and sequencing of a novel human gene that encodes a putative target protein of Nesh-SH3. J Hum Genet 46, 483–486 (2001). https://doi.org/10.1007/s100380170049
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DOI: https://doi.org/10.1007/s100380170049
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