Abstract
Rat ENH (Enigma homolog) is a LIM domain protein that associates with protein kinase C in an isoform-specific manner. We have identified a human cDNA which shares a significant sequence homology with rat ENH. The isolated cDNA clone, designated human ENH (hENH), was 3287 bp in length and encoded a predicted protein of 596 amino acids which had 88% overall identity to rat ENH protein. Northern blot analysis revealed that 1.9 kb of the hENH messenger RNA was predominantly expressed in heart and skeletal muscle, while 5.6 kb of the hENH messenger RNA was ubiquitously expressed in various human tissues. The chromosomal location of the gene was determined on chromosome 4q22 region, between markers WI-2900 and WI-3273, by polymerase chain reaction (PCR)-based analyses using both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid mapping panel.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Additional information
Received: February 25, 1999 / Accepted: April 3, 1999
Rights and permissions
About this article
Cite this article
Ueki, N., Seki, N., Yano, K. et al. Isolation, tissue expression, and chromosomal assignment of a human LIM protein gene, showing homology to rat Enigma homologue (ENH). J Hum Genet 44, 256–260 (1999). https://doi.org/10.1007/s100380050155
Published:
Issue Date:
DOI: https://doi.org/10.1007/s100380050155
This article is cited by
-
Expression of LIM domain-binding 3 (LDB3), a striated muscle Z-band alternatively spliced PDZ-motif protein in the nervous system
Scientific Reports (2023)
-
Bayesian logistic regression in detection of gene–steroid interaction for cancer at PDLIM5 locus
Journal of Genetics (2016)
-
Gene expression and association analyses of LIM (PDLIM5) in bipolar disorder and schizophrenia
Molecular Psychiatry (2005)
-
A PKCε–ENH–channel complex specifically modulates N-type Ca2+ channels
Nature Neuroscience (2003)