Summary
O 6-methylguanine-DNA methyltransferase (MGMT) is one of the DNA repair enzymes in mammals. We previously screened the variant alleles for the MGMT gene in the general population, and found three variants (V1, V2, V3), two of which caused amino acid substitutions (Leu84Phe for V1, and Trp65Cys for V2). In order to accelerate the ecogenetic and pharmacogenetic studies on MGMT polymorphism, we therefore developed a new PCR-based RFLP method for genotyping. The present method has some advantages over the initial PCR-single strand conformation polymorphism (SSCP) method, particularly regarding its simplicity, rapidity and specificity.
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Coulondre C, Miller JH (1977): Genetic studies of thelac repressor, IV. Mutagenic specificity in thelacI gene ofEscherichia coli. J Mol Biol117: 577–606
Otsuka M, Abe M, Nakabeppu Y, Sekiguchi M, Suzuki T (1996): Polymorphism in the humanO 6-methylguanine-DNA methyltransferase gene detected by PCR-SSCP analysis. Pharmacogenetics6: 361–363
Rüdiger HW, Schwartz U, Serrand E, Stief M, Krause T, Nowak D (1989): ReducedO 6-methylguanine repair in fibroblast cultures from patients with lung cancer. Cancer Res49: 5623–5626
Sambrook J, Fritsch EF, Maniatis T (1989): Isolation of DNA from mammalian cells: Protocol I. In: Ford N, Nolan C, Ferguson M (eds). Molecular cloning. A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, New York, pp 9.16–9.19
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Abe, M., Inoue, R. & Suzuki, T. A convenient method for genotyping of humanO 6-methylguanine-DNA methyltransferase polymorphism. Jap J Human Genet 42, 425–428 (1997). https://doi.org/10.1007/BF02766943
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DOI: https://doi.org/10.1007/BF02766943
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