Summary
The H19 gene is paternally imprinted both in the human and mouse (Bartolomeiet al., 1991; Zhang and Tycko, 1992), although its expression pattern seems somewhat different between the two species (Jinno,et al., 1995). DNA-methylation is a promising candidate for a parent-of-origin mark of the gene, and a paternal allele-specific methylation imprint was recently identified at the mouse H19 locus (Tremblayet al., 1995). We found a 50% methylated region in the human H19 gene (Jinno, unpublished data). A search for polymorphisms in this region revealed two novelAvaI andHhaI RFLPs, which contribute to the detection of allele-specific methylation at the human H19 locus.
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Abbreviations
- PANL2:
-
5′-GAGCCTGCCAAGCAGAGCG-3′
- PANR2:
-
5′-CACATAAGTAGGCGTGACTTGA-3′
- ASMA:
-
5′-CAATGAGGTGTCCCAGTTCCA-3′
- PANR2:
-
5′-CACATAAGTAGGCGTGACTTGA-3′
References
Bartolomei MS, Zemel S, Tilghman SM (1991): Parental imprinting of the mouse H19 gene. Nature351:153–155
Jinno Y, Ikeda Y, Yun K, Maw M, Masuzaki H, Fukuda H, Inuzuka K, Fujishita A, Ohtani Y, Okimoto T, Ishimaru T, Niikawa N (1995): Establishment of functional imprinting of the H19 gene in human developing placentae. Nature Genet10:318–324
Tremblay KD, Saam JR, Ingram RS, Tilghman SM, Bartolomei MS (1995): A paternal-specific methylation imprint marks the alleles of the mouse H19 gene. Nature Genet9:407–413
Zhang Y, Tycko B (1992): Monoallelic expression of the human H19 gene. Nature Genet1: 40–44
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Miyatake, S., Ikeda, Y., Jinno, Y. et al. Two polymorphicAvaI andHhaI sites in a differentially methylated region of the human H19 gene. Jap J Human Genet 41, 253–255 (1996). https://doi.org/10.1007/BF01875986
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DOI: https://doi.org/10.1007/BF01875986