Summary
PolymorphicN-acetyltransferase in human liver catalyzesN-acetylation of various arylamine-containing drugs and environmental chemicals. To accelerate the pharmacogenetic and ecogenetic studies ofN-acetyltransferase polymorphism, we have developed a rapid and simple method for genotyping using a polymerase chain reaction based restriction fragment length polymorphism. This method distinguishes four kinds of allele of theN-acetyltransferase gene using a single polymerase chain reaction starting with a set of primers, followed by successiveAsp718,BamHI andTaqI digestions, and then running the samples on a single electrophoresis lane. This method allows us to determine ten different genotypes easily and reliably.
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abe, M., Suzuki, T. & Deguchi, T. An improved method for genotyping ofN-acetyltransferase polymorphism by polymerase chain reaction. Jap J Human Genet 38, 163–168 (1993). https://doi.org/10.1007/BF01883706
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DOI: https://doi.org/10.1007/BF01883706
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