Abstract
An unknown impurity named amphotericin B (2) (AmB(2)) isolated from amphotericin B (AmB) bulk material was identified as (1S,3S,5R,6R,9R,11R,15S,16R,17R,18S,19E,21E,23E,25E,27E,29E,31E,33R,35S,36R,37S)-33-[(3-amino-3,6-dideoxy-β-D-mannopyranosyl)oxy]-1-methyloxy-3,5,6,9,11,17,37-heptahydroxy-15,16,18-trimethyl-13-oxo-14,39-dioxabicyclo[33.3.1]nonatriaconta-19,21,23,25,27,29,31-heptaene-36-carboxylic acid according to the IUPAC. The structure was elucidated by various 1D and 2D NMR techniques, mass spectrometry and by comparison with the NMR data of AmB. Its activity against Candida albicans was evaluated by comparison with AmB.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, J., Zhu, HQ., Li, JY. et al. Isolation, Structure Elucidation and Activity of an Unknown Impurity of Amphotericin B. J Antibiot 60, 272–276 (2007). https://doi.org/10.1038/ja.2007.34
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/ja.2007.34
Keywords
This article is cited by
-
Molecular inter-kingdom interactions of endophytes isolated from Lychnophora ericoides
Scientific Reports (2017)
-
Simultaneous determination of purity and potency of amphotericin B by HPLC
The Journal of Antibiotics (2011)
-
A newly identified derivative of amphotericin B: isolation, structure determination and primary evaluation of the activity and toxicity
The Journal of Antibiotics (2010)