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  • Original Article
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Animal Models

IgG is elevated in obese white adipose tissue but does not induce glucose intolerance via Fcγ-receptor or complement

International Journal of Obesity volume 42, pages 260–269 (2018)Cite this article

Subjects

Abstract

Background/Objectives:

In obesity, B cells accumulate in white adipose tissue (WAT) and produce IgG, which may contribute to the development of glucose intolerance. IgG signals by binding to Fcγ receptors (FcγR) and by activating the complement system. The aim of our study was to investigate whether activation of FcγR and/or complement C3 mediates the development of high-fat diet-induced glucose intolerance.

Methods:

We studied mice lacking all four FcγRs (FcγRI/II/III/IV−/−), only the inhibitory FcγRIIb (FcγRIIb−/−), only the central component of the complement system C3 (C3−/−), and mice lacking both FcγRs and C3 (FcγRI/II/III/IV/C3−/−). All mouse models and wild-type controls were fed a high-fat diet (HFD) for 15 weeks to induce obesity. Glucose metabolism was assessed and adipose tissue was characterized for inflammation and adipocyte functionality.

Results:

In obese WAT of wild-type mice, B cells (+142%, P<0.01) and IgG (+128% P<0.01) were increased compared to lean WAT. Macrophages of FcγRI/II/III/IV−/−mice released lower levels of cytokines compared to wild-type mice upon IgG stimulation. Only C3−/− mice showed reduced HFD-induced weight gain as compared to controls (−18%, P<0.01). Surprisingly, FcγRI/II/III/IV−/− mice had deteriorated glucose tolerance (AUC +125%, P<0.001) despite reduced leukocyte number (−30%, P<0.05) in gonadal WAT (gWAT), whereas glucose tolerance and leukocytes within gWAT in the other models were unaffected compared to controls. Although IgG in gWAT was increased (+44 to +174%, P<0.05) in all mouse models lacking FcγRIIb, only FcγRI/II/III/IV/C3−/− mice exhibited appreciable alterations in immune cells in gWAT, for example, increased macrophages (+36%, P<0.001).

Conclusions:

Lack of FcγRs reduces the activity of macrophages upon IgG stimulation, but neither FcγR nor C3 deficiency protects against HFD-induced glucose intolerance or reduces adipose tissue inflammation. This indicates that if obesity-induced IgG contributes to the development of glucose intolerance, this is not mediated by FcγR or complement activation.

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Acknowledgements

We thank Fatiha el Bouazzaoui, Mattijs Heemskerk, Jill Claassens (Department of Human Genetics, LUMC, Leiden, The Netherlands), Marcel Camps (Department of Immunohematology and Blood Transfusion, LUMC, Leiden, The Netherlands), Lianne van der Wee-Pals and Trea Streefland (Department of Medicine, Division of Endocrinology, LUMC, Leiden, The Netherlands) for their excellent technical assistance. This work was supported by grants from the Center of Medical Systems Biology (CMSB), the Netherlands Consortium for Systems Biology (NCSB) established by the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research (NGI/NWO), the Leiden University Medical Center and Rembrandt Institute of Cardiovascular Science (RICS). Jan Van den Bossche received a VENI grant from ZonMW (91615052) and a Netherlands Heart Foundation Junior Postdoctoral grant (2013T003). MRB is supported by the Dutch Diabetes Foundation (grant 2015.81.1808). PCNR is an Established Investigator of the Netherlands Heart Foundation (grant 2009T038).

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Author notes

  1. A D van Dam and L van Beek: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Medicine, Division of Endocrinology, Leiden University Medical Center, Leiden, The Netherlands

    A D van Dam, P C N Rensen, M R Boon & K Willems van Dijk

  2. Einthoven Laboratory for Experimental Vascular Medicine, Leiden, The Netherlands

    A D van Dam, L van Beek, A C M Pronk, P C N Rensen, M R Boon, K Willems van Dijk & V van Harmelen

  3. Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands

    L van Beek, A C M Pronk, J S Verbeek, K Willems van Dijk & V van Harmelen

  4. Department of Medical Biochemistry, Subdivision of Experimental Vascular Biology, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands

    S M van den Berg, J Van den Bossche & M P J de Winther

  5. Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands

    F Koning

  6. Department of Medicine, Division of Nephrology, Leiden University Medical Center, Leiden, The Netherlands

    C van Kooten

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Correspondence to V van Harmelen.

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van Dam, A., van Beek, L., Pronk, A. et al. IgG is elevated in obese white adipose tissue but does not induce glucose intolerance via Fcγ-receptor or complement. Int J Obes 42, 260–269 (2018). https://doi.org/10.1038/ijo.2017.209

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