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Animal Models

Duodenal endoluminal barrier sleeve alters gut microbiota of ZDF rats

International Journal of Obesity volume 41, pages 381–389 (2017)Cite this article

Subjects

Abstract

Background/Objectives:

The combination of energy dense diets and reduced energy expenditure in modern society has escalated the prevalence of obesity and obesity-related comorbidities. Among these disease states, type-2 diabetics (T2D) are disproportionately associated with obesity, suggesting a shared etiology. In conjunction with defects in hormonal and inflammatory states, obesity and T2D are also characterized by dysbiosis.

Methods:

We have recently described the beneficial effects of duodenal nutrient exclusion, as induced by the duodenal endoluminal sleeve (DES); including body weight loss, prevented fat mass accumulation, and improved glucose tolerance in the ZDF rat, a rodent model of obesity and type-2 diabetes (T2D). To assess the relative role of DES on hindgut microbiota in the context of these metabolic changes, we analyzed cecal samples from rats implanted with a duodenal endoluminal sleeve (DES), or a sham control of this procedure. A group of pair-fed (pf) sham controls was also included to account for changes induced by reduced body weight and food intake.

Results:

Analysis of hindgut microbiota following DES in the ZDF rat elucidated discrete changes in several microbial populations including a reduction in Paraprevotella family members of the Clostridiales order along with an increase in Akkermansia muciniphila and species of the Allobaculum and Bifidobacterium genera.

Conclusions:

Altogether, these observations suggest that like Roux-en Y gastric bypass (RYGB) and Metformin, regulation of gut microbiota may be a contributing factor to the therapeutic effects of DES.

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Acknowledgements

We thank Peter Eipers for sample processing and 16S analysis. The project described was supported by Award Number 5K01DK098319 (KMH) from the National Institute Of Diabetes And Digestive And Kidney Diseases. The following are acknowledged for their support of the Microbiome Resource at the University of Alabama at Birmingham: School of Medicine, Comprehensive Cancer Center (P30AR050948), Center for AIDS Research (5P30AI027767), Center for Clinical Translational Science (UL1TR000165) and Heflin Center.

Author contributions

TK and KMH were responsible for study conception and design, data analyses and interpretation, and drafting the article; TK and CLH generated experimental data; TP and CDM advised study concept, data analyses, and critical revision of the article. KMH is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

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Authors and Affiliations

  1. Comprehensive Diabetes Center and Department of Medicine—Endocrinology, Diabetes and Metabolism, University of Alabama at Birmingham, Birmingham, AL, USA

    T Kim, C L Holleman & K M Habegger

  2. Center for Clinical and Translational Sciences, University of Alabama at Birmingham, Birmingham, AL, USA

    T Ptacek

  3. Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA

    T Ptacek & C D Morrow

  4. Department of Cell Developmental and Integrative Biology, University of Alabama at Birmingham, Birmingham, AL, USA

    C D Morrow

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Correspondence to K M Habegger.

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Kim, T., Holleman, C., Ptacek, T. et al. Duodenal endoluminal barrier sleeve alters gut microbiota of ZDF rats. Int J Obes 41, 381–389 (2017). https://doi.org/10.1038/ijo.2016.224

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