Abstract
Polyphenolic compounds, such as resveratrol, have recently received widespread interest because of their ability to mimic effects of calorie restriction. The objective of the present study was to gain more insight into the effects of 30 days resveratrol supplementation on adipose tissue morphology and underlying processes. Eleven healthy obese men were supplemented with placebo and resveratrol for 30 days (150 mg per day), separated by a 4-week washout period in a double-blind randomized crossover design. A postprandial abdominal subcutaneous adipose tissue biopsy was collected to assess adipose tissue morphology and gene expression using microarray analysis. Resveratrol significantly decreased adipocyte size, with a shift toward a reduction in the proportion of large and very-large adipocytes and an increase in small adipocytes. Microarray analysis revealed downregulation of Wnt and Notch signaling pathways and upregulation of pathways involved in cell cycle regulation after resveratrol supplementation, suggesting enhanced adipogenesis. Furthermore, lysosomal/phagosomal pathway and transcription factor EB were upregulated reflecting an alternative pathway of lipid breakdown by autophagy. Further research is necessary to investigate whether resveratrol improves adipose tissue function.
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References
Harris MI, Flegal KM, Cowie CC, Eberhardt MS, Goldstein DE, Little RR et al. Prevalence of diabetes, impaired fasting glucose, and impaired glucose tolerance in U.S. adults. The Third National Health and Nutrition Examination Survey, 1988-1994. Diabetes Care 1998; 21: 518–524.
Szkudelska K, Szkudelski T . Resveratrol, obesity and diabetes. Eur J Pharmacol 2010; 635: 1–8.
Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, Kalra A et al. Resveratrol improves health and survival of mice on a high-calorie diet. Nature 2006; 444: 337–342.
Kim S, Jin Y, Choi Y, Park T . Resveratrol exerts anti-obesity effects via mechanisms involving down-regulation of adipogenic and inflammatory processes in mice. Biochem Pharmacol 2011; 81: 1343–1351.
McLaughlin T, Sherman A, Tsao P, Gonzalez O, Yee G, Lamendola C et al. Enhanced proportion of small adipose cells in insulin-resistant vs insulin-sensitive obese individuals implicates impaired adipogenesis. Diabetologia 2007; 50: 1707–1715.
Poulsen MM, Vestergaard PF, Clasen BF, Radko Y, Christensen LP, Stodkilde-Jorgensen H et al. High-dose resveratrol supplementation in obese men: an investigator-initiated, randomized, placebo-controlled clinical trial of substrate metabolism, insulin sensitivity, and body composition. Diabetes 2013; 62: 1186–1195.
Yoshino J, Conte C, Fontana L, Mittendorfer B, Imai S, Schechtman KB et al. Resveratrol supplementation does not improve metabolic function in nonobese women with normal glucose tolerance. Cell Metab 2012; 16: 658–664.
Timmers S, Konings E, Bilet L, Houtkooper RH . van de Weijer T, Goossens GH et al. Calorie restriction-like effects of 30 days of resveratrol supplementation on energy metabolism and metabolic profile in obese humans. Cell Metab 2011; 14: 612–622.
Goossens GH, Bizzarri A, Venteclef N, Essers Y, Cleutjens JP, Konings E et al. Increased adipose tissue oxygen tension in obese compared with lean men is accompanied by insulin resistance, impaired adipose tissue capillarization, and inflammation. Circulation 2011; 124: 67–76.
Settembre C, Di Malta C, Polito VA, Garcia Arencibia M, Vetrini F, Erdin S et al. TFEB links autophagy to lysosomal biogenesis. Science 2011; 332: 1429–1433.
Goossens GH . The role of adipose tissue dysfunction in the pathogenesis of obesity-related insulin resistance. Physiol Behav 2008; 94: 206–218.
Christodoulides C, Laudes M, Cawthorn WP, Schinner S, Soos M, O'Rahilly S et al. The Wnt antagonist Dickkopf-1 and its receptors are coordinately regulated during early human adipogenesis. J Cell Sci 2006; 119 (Pt 12): 2613–2620.
Garces C, Ruiz-Hidalgo MJ, Font de Mora J, Park C, Miele L, Goldstein J et al. Notch-1 controls the expression of fatty acid-activated transcription factors and is required for adipogenesis. J Biol Chem 1997; 272: 29729–29734.
Picard F, Kurtev M, Chung N, Topark-Ngarm A, Senawong T, Machado De Oliveira R et al. Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-gamma. Nature 2004; 429: 771–776.
Fischer-Posovszky P, Kukulus V, Tews D, Unterkircher T, Debatin KM, Fulda S et al. Resveratrol regulates human adipocyte number and function in a Sirt1-dependent manner. Am J Clin Nutr 2010; 92: 5–15.
He Q, Gao Z, Yin J, Zhang J, Yun Z, Ye J . Regulation of HIF-1{alpha} activity in adipose tissue by obesity-associated factors: adipogenesis, insulin, and hypoxia. Am J Physiol Endocrinol Metab 2011; 300: E877–E885.
Brakenhielm E, Cao R, Cao Y . Suppression of angiogenesis, tumor growth, and wound healing by resveratrol, a natural compound in red wine and grapes. FASEB J 2001; 15: 1798–1800.
Singh R, Cuervo AM . Lipophagy: connecting autophagy and lipid metabolism. Int J Cell Biol 2012; 2012: 282041.
Singh R, Xiang Y, Wang Y, Baikati K, Cuervo AM, Luu YK et al. Autophagy regulates adipose mass and differentiation in mice. J Clin Invest 2009; 119: 3329–3339.
Siklova-Vitkova M, Klimcakova E, Polak J, Kovacova Z, Tencerova M, Rossmeislova L et al. Adipose tissue secretion and expression of adipocyte-produced and stromavascular fraction-produced adipokines vary during multiple phases of weight-reducing dietary intervention in obese women. J Clin Endocrinol Metab 2012; 97: E1176–E1181.
Acknowledgements
We thank Jos Stegen and Yvonne Essers for their excellent technical assistance with the biochemical analysis and adipocyte cell size analysis. This study was funded by Top Institute Food and Nutrition. TI Food and Nutrition, formerly known as WCFS, is a unique public/private partnership that generates vision on scientific breakthroughs in food and nutrition, resulting in the development of innovative products and technologies that respond to consumer demands for safe, tasty and healthy foods. Partners are major Dutch food companies and research organizations. We also thank DSM Nutritional Products Ltd., Kaiseraugst, Switzerland for providing us with the resVidaTM and placebo capsules.
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Konings, E., Timmers, S., Boekschoten, M. et al. The effects of 30 days resveratrol supplementation on adipose tissue morphology and gene expression patterns in obese men. Int J Obes 38, 470–473 (2014). https://doi.org/10.1038/ijo.2013.155
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DOI: https://doi.org/10.1038/ijo.2013.155
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