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  • Original Article
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Knockdown of angiotensinogen by shRNA-mediated RNA interference inhibits human visceral preadipocytes differentiation

International Journal of Obesity volume 34, pages 157–164 (2010)Cite this article

A Corrigendum to this article was published on 10 December 2013

Abstract

Objective:

The immediate cause of obesity is the massive deposition of subcutaneous and visceral fat attributing to the continuous proliferation and differentiation of preadipocytes. The identification of the underlying molecular mechanisms of preadipocytes differentiation is urgent, and will have an important role in plastic and reconstructive surgical procedures.

Methods:

Two small hairpin RNA (shRNA)-mediated RNA interference plasmids have been constructed on the basis of the activity of H1 promoter-driven expression vector psiRNA-hH1neo to suppress the expression of angiotensinogen (AGT) in human preadipocytes-visceral (HPA-v). Subsequently, glycerol-3-phosphate dehydrogenase (G3PDH) activity and intracytoplasmic lipids content were detected during the process of HPA-v differentiation.

Results:

Small hairpin RNA-expressing vectors have been successfully constructed to suppress the expression of AGT significantly. Both intracytoplasmic lipids content and G3PDH activity decreased to a certain extent compared with that in the control group in the whole process of HPA-v differentiation.

Conclusions:

Two shRNA-mediated AGT-targeting plasmids inhibited the process of HPA-v differentiation to a certain extent. However, the accumulation of intracytoplasmic lipids was not exclusively determined by the expression of AGT, and it may also be regulated by other factors. In conclusion, this study provided a method to inhibit the process of preadipocytes differentiation, and it may have a role in obesity treatment and adipose tissue engineering application.

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Acknowledgements

This project was supported by the National Natural Foundation of China (Grant 30870025).

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  1. College of Food and Bioengineering, South China University of Technology, Guangzhou, China

    Z-W Ye, X-M Wu & J-G Jiang

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  1. Z-W Ye
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Correspondence to J-G Jiang.

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Ye, ZW., Wu, XM. & Jiang, JG. Knockdown of angiotensinogen by shRNA-mediated RNA interference inhibits human visceral preadipocytes differentiation. Int J Obes 34, 157–164 (2010). https://doi.org/10.1038/ijo.2009.197

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