Abstract
Experimental and clinical observations suggest that E-selectin could have an important role in essential hypertension (EH), but the relationship between common E-selectin variants and EH has not been extensively studied in the Chinese population. In this study, we explored the association between two common variants in the E-selectin gene (rs5361A/C and rs5355C/T) and EH in the Uygur, Kazakh and Han populations in the Xinjiang area. A case-control study was conducted to explore the association between these two single-nucleotide polymorphisms and EH in a large sample size, including 941 EH subjects (309 Uygur, 264 Kazakh and 368 Han individuals) and 924 control subjects (300 Uygur, 275 Kazakh and 349 Han individuals). Univariate analysis showed that the rs5361 A/C polymorphism was significantly associated with EH in the Uygur (P=0.002) and Han (P=3.6 × 10−7) populations. The CC genotype of this SNP was present only in patients with EH in all of the three nationalities studied. Han individuals who carry the CC genotype of rs5361 were more susceptible to EH, according to the dominant models (P=1.13 × 10−4, odds ratio=3.812, 95% confidence interval: 1.685–7.792), but there was no association of genotype with EH for the other ethnicities. No significant difference in rs5355 C/T polymorphism rate was found between the EH and control groups. Our results indicate that the common variant rs5361 is strongly associated with EH in Han individuals and weakly associated in Uygur individuals. The CC genotype of rs5361 might be an independent risk factor for EH among Uygur, Kazakh and Han individuals in the Xinjiang area.
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Introduction
Essential hypertension (EH) is one of the largest public health issues threatening human health worldwide. The morbidity and mortality from EH-induced coronary heart disease, stroke and renal events account for an increasingly large portion of all morbidity and mortality each year. It has been estimated that by 2020, coronary heart disease and stroke will be the first and fourth most common causes of morbidity worldwide, respectively. Human E-selectin, a proinflammatory cytokine also known as the leukocyte differentiation antigen CD62E, is closely associated with EH. The gene encoding E-selectin is located on the long arm of chromosome 1, and it contains 14 exons and 13 introns spanning ∼13 kilobases of the human genome. Recently, several E-selectin gene polymorphisms were suggested to be associated with EH.1, 2 Marteau et al.3 found that the Leu554Phe polymorphism (rs5355) in E-selectin is associated with elevated blood pressure (BP) in overweight people. An age-specific effect of this polymorphism on BP levels was also suggested by Sass et al.4 Wenzel et al.5 revealed that two mutations, Ser128Arg and Leu554Phe, could influence E-selectin function in vitro and may be considered as risk factors in the complex pathogenesis of atherosclerosis. It has also been suggested that the Ser128Arg polymorphism can functionally alter leukocyte-endothelial interactions, and may have other biochemical and biological consequences that are relevant to the pathogenesis of myocardial infarction.6 On the basis of these results, we hypothesized that rs5361 may also have a role in the pathogenesis of hypertension. Therefore, we genotyped two single-nucleotide polymorphisms in E-selectin, rs5361 A/C and rs5355 C/T, using TaqMan allelic discrimination assays (Shanghai GeneCore BioTechnologies Co., Ltd., Shanghai, China) to explore the association between these polymorphisms and EH among Uygur, Kazakh and Han individuals in the Xinjiang area.
Methods
Subjects
A total of 941 individuals, aged 30–76 years at recruitment (309 Uygur, 264 Kazakh and 368 Han), were enrolled in an epidemiological survey focusing on hypertension prevalence that was launched in four geographical regions within the Xinjiang province of China in 2008. The control group, consisting of 924 unrelated individuals (300 Uygur, 275 Kazakh and 349 Han), was initially recruited in the same study and matched to cases by sex, race, geographic location and date of collection (within 3 months). All participants underwent standard medical history and physical evaluations, including an assessment of smoking history, stroke, diabetes, coronary artery disease status and the use of antihypertensive medications within 2 weeks of the epidemiological survey. Plasma glucose, total cholesterol, high-density lipoprotein cholesterol and triglyceride levels were measured using standard methods with an OLYMPUS AU 2700 automatic biochemical analyzer (Olympus CO Ltd., Tokyo, Japan), after the patients had fasted for 12 h overnight. The low-density lipoprotein cholesterol concentration was estimated by the Friedewald formula.7 EH was defined as a systolic BP ⩾140 mm Hg or diastolic BP ⩾90 mm Hg in the absence of treatment with antihypertensive drugs. Subjects with EH who did not receive any antihypertensive therapy in the 2 weeks before the epidemiological survey and developed neither complications nor clinical manifestations were included in this study. Participants were excluded from analyses if they (1) had pre-hypertension or received any antihypertensive medication, (2) had severe hepatic or renal dysfunction, secondary hypertension or severe heart failure or (3) were pregnant, lactating or taking oral contraceptives. The local bioethics committee approved the study protocol, and informed consent was obtained from all participants prior to their participation in the study.
DNA extraction and genotyping
Fasting venous blood was collected in 5 ml EDTA tubes, and genomic DNA was isolated with a DNA extraction kit (FUJIFILM, Tokyo, Japan) according to the manufacturer's protocol. Genotyping was performed with the TaqMan SNP allelic discrimination assay. TaqMan Universal PCR Master Mix (2 × ) was obtained from Applied Biosystems (Carlsbad, CA, USA). Fluorescent probes and PCR amplification primers for the two alleles were designed and synthesized by Shanghai GeneCore BioTechnologies (Shanghai, China). All fluorescent probes were TaqMan TAMRA probes consisting of a TAMRA modifying group, a 5′-reporter dye and a 3′-non-fluorescent quencher. The probe and primer sequences are listed in Table 1.
PCR amplification system, reaction conditions and genotype analysis
Amplifications were performed in an ABI 7900HT thermal cycler (Applied Biosystems) with a PCR temperature profile consisting of incubation at 50 °C for 2 min, denaturation at 95 °C for 10 min, and 50 cycles of denaturation at 95 °C for 15 s and annealing at 60 °C for 60 s. Each PCR amplification reaction contained 5 μl of 1 × Universal Master Mix (Applied Biosystems) containing 0.2 μM each of two probes, 1 μM each primer and 1 ng μl−1 genomic DNA. The genotypes of the two alleles were differentiated with SDS 2.1 software (Applied Biosystems) (Supplementary Figure 1), and 10% of all genotyping reactions were repeated in independent PCRs to check for consistency and to ensure intraplate and interplate reproducibility. No discrepancies were detected between the repeated samples. In addition, all paired case and control DNA samples were run in the same batches.
Statistical analysis
Statistical analysis was conducted with SPSS 13.0 (SPSS Inc., Chicago, IL, USA). Continuous variable data that fit a normal distribution were expressed as the mean±s.d. χ2-tests were employed to test whether the genotypes were in Hardy-Weinberg equilibrium, and were also used to compare the genotype and allele frequencies between the EH and control groups. All analyses were performed separately by race. Unconditional logistic regression was performed to estimate the odds ratios (OR) and 95% confidence intervals (CI). Covariates included gender, age, body mass index, smoking status, diabetes, stroke and coronary artery disease. The study had an 80% power to detect an association with an OR>2.80 (assuming a risk effect) for minor alleles at a 1.4% frequency, assuming a two-sided significance level at 0.05 and a dominant gene model. The QUANTO program (developed by Jim Gauderman and John Morrison, http://hydra.usc.edu/gxe) was used to construct the model.8
Results
Subject characteristics
Demographic details for the case and control groups are given in Table 2. There were no significant differences in age or gender between case and control groups. In all three ethnicities, systolic BP and diastolic BP were higher among cases than among controls. The prevalence of coronary artery disease (CAD) and hyperlipidemia was higher in hypertensive subjects than in controls in the Uygur population. The hypertensive group contained a higher proportion of smokers in the Han population but not in the other two populations. Significantly higher serum total cholesterol, triglyceride (TG) and low-density lipoprotein levels were identified in the case group for all three ethnicities. However, higher concentrations of fasting glucose were observed only in Han hypertensive subjects, whereas higher high-density lipoprotein levels were seen in Kazakh and Uygur hypertensive subjects. The genotype distributions of the two single-nucleotide polymorphisms (rs5361 and rs5355) were in Hardy-Weinberg equilibrium in all control groups, and the minor allele frequencies in the controls were very similar to those published for the Han population in the NCBI database (Table 3), indicating that the selected group was representative of the population as a whole.
Association analysis
Association statistics results are listed in Table 4. Univariate analysis showed that rs5361 A/C polymorphism was significantly associated with EH in the Uygur (P=0.002) and Han (P=3.6 × 10−7) populations. No significant correlation was found in the Kazakh population (P=0.172). The CC genotype of rs5361 was found only in the EH group in all three nationalities, with frequencies of 4.6%, 0.4% and 3.5% for the Uygur, Kazakh and Han populations, respectively. Multivariate unconditional logistic regression analysis showed that rs5361 polymorphism was significantly associated with EH, independent of traditional cardiovascular risk factors, in a dominant model in the Han cohort (P=1.13 × 10−4, OR=3.81, 95% CI: 1.685–7.792). No effect was identified in the other two ethnicities.
For rs5355 C/T, no significant correlation was found between gene polymorphism and EH for any of the three nationalities (P=0.077 for Uygur, P=0.27 for Kazakh and P=0.635 for Han) in the univariate analyses. Additionally, multivariate logistic regression using a dominant model did not reveal any significant relationship between rs5355 polymorphism and EH in these three ethnicities.
Discussion
In 2001, Bellelli et al.9 reported that the serum level of high-sensitive C-reactive protein was an independent risk factor for EH. It has generally been thought that inflammatory injury of the vascular endothelial tissue has an important role in the pathology of EH, and E-selectin-mediated leukocyte adhesion and aggregation is thought to be a prerequisite for inflammatory injury of the vascular endothelia. Although the inflammatory response in the vascular endothelia can be mediated by many kinds of adhesion molecules, the role of E-selectin cannot be performed by other adhesion molecules, and E-selectin is therefore essential for this response.10
E-selectin was first identified in 1985 as a transmembrane glycoprotein that is synthesized by vascular endothelial cells after their activation by inflammatory factors and then released from the cell surface as a proinflammatory cytokine. E-selectin was later found to mediate leukocyte adhesion to endothelial cells and their subsequent penetration of the vascular wall.11 It is now clear that inflammatory factors affect BP by inducing the expression of adhesion molecules, mainly E-selectin, in endothelial tissue. Generally, E-selectin induces the adhesion and aggregation of leukocytes, which subsequently migrate into the vascular wall or extravascular space, thus injuring vascular endothelial cells, impairing endothelium-dependent vascular relaxation and finally leading to hypertension. Conversely, hypertension also contributes to inflammatory responses through hemodynamic mechanisms.12 Thus, hypertension escalates through a positive feedback loop in which inflammation and hypertension promote one another.13 Furthermore, hypertension induced by E-selectin might also be related to microcirculation abnormalities; changes in microcirculation structure and function might result from and subsequently contribute to a high BP.14 Hypertension is accompanied by a series of morphological changes, such as increased wall-to-lumen area ratios in small resistance arteries and decreased capillary network densities in the vascular bed,15 which might maintain and enhance BP by increasing peripheral resistance. E-selectin has also been found to impair microcirculation integrity. By promoting the adhesion of leukocytes to endothelial cells and leukocyte-endothelia interactions, enhancing the production of cytokines as well as the products of cytokine-catalyzed reactions in migrated and aggregated leukocytes,16 E-selectin leads to changes in microvessels, such as the reconstruction of smooth muscle cells, finally affecting BP. Meanwhile, E-selectin-mediated injury of the vascular endothelia induces several complications of hypertension, such as atherosclerotic vascular disease. This is consistent with the established relationship between E-selectin gene polymorphism or serum soluble E-selectin level and atherosclerotic vascular disease, such as coronary heart disease, ischemic stroke and kidney injury.6, 17, 18 Therefore, E-selectin is intricately involved in the etiology of hypertension.
Chen HL et al.19 found that individuals with a C allele at rs5361 have a relatively higher risk of hypertension, higher diastolic BP and a higher mean arterial pressure. Wang et al.20 also suggested that the C allele at this locus was more frequent among hypertensive individuals, consistent with the results of our study. Meanwhile, the rs5355 C/T polymorphism in the E-selectin gene showed age-specific effects on BP in another study.2 However, these conclusions were not fully confirmed. Our study demonstrated that this polymorphism was not significantly correlated with EH in the Uygur, Kazakh or Han populations in Xinjiang area, whereas rs5361 A/C was significantly correlated with EH in the Han population but only mildly in the Uygur population (P=3.6 × 10−7 and P=0.002, respectively). Subjects carrying the C allele were more susceptible to EH under the dominant models (P=1.13 × 10−4, OR=3.812, 95% CI: 1.685–7.792) in the Han population, but this effect was not observed in the other ethnicities. This difference could be attributed to the genetic heterogeneity among these different populations. Some variants may be poor markers for the trait because of differences in linkage-disequilibrium structure in ethnic backgrounds. As no data on linkage-disequilibrium patterns for Uygur and Kazakh populations are available through the HapMap database, we cannot rule out the possibility that there are differences between these groups and the Han population. Furthermore, the minor allele frequency of these two single-nucleotide polymorphisms differed significantly between the Han population and the other two populations (Table 4). It has been shown that even a small change in the minor allele frequency of an SNP can dramatically reduce a study's power to replicate a main effect for the same SNP.21 In addition, our sample sizes of Uygur and Kazakh individuals might be not large enough to reliably detect the effect observed in the Han population. More importantly, the risks in the development of hypertension in these ethnicities may be different because of true etiologic heterogeneity, which may be driven by differences in gene-gene or gene-environment interactions. This hypothesis is supported by the fact that the EH in Kazakh people in the Xinjiang area is characterized by high morbidity and is significantly affected by environment and diet,20 and that, among the elderly, non-Han individuals suffer from a greater prevalence of hypertension and a higher body mass index than Han individuals.22
The CC genotype at rs5361 was found only in EH patients, with frequencies of 4.6%, 0.4% and 3.5% for Uygur, Kazakh and Han populations, respectively. These findings suggest that the CC genotype of E-selectin allele rs5361 A/C might be an independent risk factor for EH in Uygur, Kazakh and Han populations in the Xinjiang area.
In summary, our study found a strong association between rs5361 polymorphism and EH in the Han population and a weak association in the Uygur population; our results also indicate that the CC genotype might be an independent risk factor for EH in Uygur, Kazakh and Han individuals in the Xinjiang area. Further research, specifically large-scale prospective cohort studies and further investigation of the biological mechanism underlying the link between E-selectin and essential hypertension, will be needed to confirm these findings.
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Acknowledgements
This work was supported by grants from the Medical and Health Care Science & Technology Specific Projects of Xinjiang Production and Construction Corps (no. 2008GG39).
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Wang, Z., Xu, Y., Chen, S. et al. A common missense single nucleotide polymorphism in the E-selectin gene is significantly associated with essential hypertension in the Han population but only weakly associated in the Uygur population. Hypertens Res 35, 413–417 (2012). https://doi.org/10.1038/hr.2011.204
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DOI: https://doi.org/10.1038/hr.2011.204
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