Abstract
This work examines the nature of north-south clinal variation in morphological characters in Drosophila melanogaster. Isofemale lines were established from flies collected along a transect extending from Winnipeg, Manitoba (Canada) to Tampa Bay, Florida (U.S.A.). Offspring from different lines within each location were then cultured under standardized conditions and used to examine phenotypic variation in seven morphological characters along the cline. In addition, allozyme variation at seven polymorphic loci was examined for the same set of clinal populations. Scutellum length and wing length show the strongest clinal trends. Clinal variation is nonmonotonic, with larger flies in the middle latitudes and smaller flies in the north and south. This result contrasts with other studies which have shown monotonic clines. Patterns of population subdivision were different for the different characters. This implies that there are different selective forces acting on the different morphological characters. Based on a comparison of morphological and molecular population subdivision for adjacent populations it is inferred that natural selection is operating to maintain a high level of population subdivision for wing width and the first principal component between one of the sets of populations. A combined approach using molecules and morphology may provide an alternative to retrospective selection analysis for detecting selection in nature.
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References
Anderson, W W. 1966. Genetic divergence in M. Vetukhiv's experimental populations of Drosophila pseudoobscura. 3. Divergence in body size. Genet Res, 7, 255–266.
Anderson, W W. 1973. Genetic divergence in body size among experimental populations of Drosophila pseudoobscura kept at different temperatures. Evolution, 27, 278–284.
Ashburner, M. 1989. Drosophila: A Laboratory Handbook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Barton, N H, and Turelli, M. 1989. Evolutionary quantitative genetics: how little do we know? Ann Rev Genet, 23, 337–370.
Berry, A, and Kreitman, M. 1993. Molecular analysis of an allozyme cline: alcohol dehydrogenase in Drosophila melanogaster on the east coast of North America. Genetics, 134, 869–893.
Chatfield, C, and Collins, A J. 1980. Introduction to Multivariate Analysis. Chapman and Hall, New York.
Coyne, J A, and Beecham, E. 1987. Heritability of two morphological characters within and among natural populations of Drosophila melanogaster. Genetics, 117, 727–737.
Coyne, J A, Bryant, S N, and Turelli, M A. 1987. Longdistance migration of Drosophila. 2. Presence in desolate sites and dispersal near a desert oasis. Am Nat, 129, 847–861.
Coyne, J A, Bundgaard, J, and Prout, T. 1983. Geographic variation of tolerance to environmental stress in Drosophila pseudoobscura. Am Nat, 122, 474–488.
Coyne, J A, and Milstead, B. 1987. Long-distance migration of Drosophila. 3. Dispersal of D. melanogaster alleles from a Maryland orchard. AM Nat, 130, 70–82.
Crow, J F, and Kimura, M. 1970. An Introduction to Population Genetics Theory. Burgess Publishing Company, Minneapolis, MN.
David, J R, and Capy, P. 1988. Genetic variation of Drosophila melanogaster natural populations. Trends Genet, 4, 106–111.
Endler, J A. 1977. Geographic Variation, Speciation, and Clines Monographs in Population Biology. Princeton University Press, Princeton, NJ.
Falconer, D S. 1981. Introduction to Quantitative Genetics, 2nd edn. Longman, New York.
Gupta, A P, and Lewontin, R C. 1982. A study of reaction norms in natural populations of Drosophila pseudoobscura. Evolution, 36, 934–948.
Hebert, P D N, and Beaton, M J. 1989. Methodology for Allozyme Analysis using Cellulose Acetate Electrophoresis. Helena Laboratories, Beaumont, TX.
Hoffman, A A, and Nielsen, K K. 1985. The effect of resource allocation on genetic variation in Drosophila. Am Nat, 125, 421–430.
Hyytia, P, Capy, P, David, J R, and Singh, R S. 1985. Enzymatic and quantitative variation in European and African populations of Drosophila simulans. Heredity, 54, 209–217.
James, F C. 1983. Environmental component of morphological differentiation in birds. Science, 221, 184–186.
Lachaise, D, Cariou, M-L, David, J R, Lemeunier, F, Tsacas, L, and Ashburner, M. 1988. Historical biogeography of the Drosophila melanogaster species subgroup. Evol Biology, 22, 159–225.
Lande, R. 1979. Quantitative genetic analysis of multivariate evolution, applied to brain: body size allometry. Evolution, 33, 402–416.
Lande, R, and Arnold, S J. 1983. The measurement of selection on correlated characters. Evolution, 37, 1210–1226.
Levins, R A. 1969. Thermal acclimation and heat resistance in Drosophila species. Am Nat, 103, 483–499.
Lewontin, R C, and Krakauer, J. 1973. Distribution of gene frequency as a test of the theory of the selective neutrality of polymorphisms. Genetics, 74, 175–195.
Long, A D. 1991. Quantitative Genetics of Clinal Variation MSC. Thesis, McMaster University.
Misra, R K, and Reeve, E C R. 1964. Clines in body dimensions in populations of Drosophila subobscura. Genet Res, 5, 240–256.
Powell, J R. 1974. Temperature related genetic divergence in Drosophila body size. J Hered, 65, 257–258.
Price, P T, Grant, P R, Gibbs, H L, and Boag, P T. 1984. Recurrent patterns of natural selection in a population of Darwin's finches. Nature, 309, 787–789.
Prout, T. 1958. A possible difference in genetic variance between wild and laboratory populations. Drosoph Inf Serv, 32, 148–149.
Prout, T, and Barker, J S F. 1993. F statistics in Drosophila buzzatii: selection, population size and inbreeding. Genetics, 134, 369–375.
Reyment, R A, Blackith, R C, and Campbell, N A. 1984. Multivariate Morphometries, 2nd edn. Academic Press, Toronto.
Robertson, A. 1975. Gene frequency distributions as a test of selective neutrality. Genetics, 81, 775–785.
Robertson, F W. 1957. Studies in quantitative inheritance. XI. Genetic and environmental correlations between body size and egg production in Drosophila melanogaster. J Genet, 55, 428–443.
Singh, R S. 1989. Population genetics and evolution of species related to Drosophila melanogaster. Ann Rev Genet, 23, 425–453.
Singh, R S, and Long, A D. 1992. Geographic variation in Drosophila: from molecules to morphology and back. Trends Ecol Evol, 7, 340–345.
Singh, R S, and Rhomberg, L R. 1987a. A comprehensive study of genie variation in natural populations of Drosophila melanogaster. I. Estimates of gene flow from rare alleles. Genetics, 115, 313–322.
Singh, R S, and Rhomberg, L R. 1987b. A comprehensive study of genie variation in natural populations of Drosophila melanogaster. II. Estimates of heterozygosity and patterns of geographic differentition. Genetics, 117, 255–271.
Sokal, R S, and Rolf, F J. 1981. Biometry, 2nd edn. W. F. Freeman and Co., San Francisco.
Sokoloff, A. 1965. Geographic variation in quantitative characters in populations of Drosophila pseudoobscura. Evolution, 19, 300–310.
Spitze, K. 1993. Population structure in Daphnia obtusa: quantitative genetic and allozymic variation. Genetics, 135, 367–374.
Stalker, H D, and Carson, H L. 1947. Morphological variation in natural populations of Drosophila robusta Sturtevant. Evolution, 1, 237–248.
Stalker, H D, and Carson, H L. 1948. An altitudinal transect of Drosophila robusta Sturtevant. Evolution, 2, 295–305.
Stalker, H D, and Carson, H L. 1949. Seasonal variation in the morphology of Drosophila robusta Sturtevant. Evolution, 3, 330–343.
Turelli, M. 1988. Phenotypic evolution, constant covariances, and the maintenance of additive variation. Evolution, 42, 1342–1347.
Watada, M, Ohba, S, and Tobari, Y N. 1986. Genetic differentiation in Japanese populations of Drosophila simulans and D. melanogaster. Jap J Genet, 61, 469–480.
Wright, S. 1931. Evolution in Mendelian populations. Genetics, 16, 97–159.
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Long, A., Singh, R. Molecules versus morphology: the detection of selection acting on morphological characters along a cline in Drosophila melanogaster. Heredity 74, 569–581 (1995). https://doi.org/10.1038/hdy.1995.81
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DOI: https://doi.org/10.1038/hdy.1995.81
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