Abstract
A genomic probe encompassing most of an esterase gene (E4) that is amplified in insecticide-resistant Myzus persicae was hybridized in situ to mitotic and meiotic chromosome preparations of aphid clones of known esterase type and resistance level. Binding, which was detected using the biotin-avidin system located both known types of amplified esterase sequences (E4 and FE4). All except one of the E4-producing clones had a single amplified site, on autosome 3 near the breakpoint of an autosomal 1,3 translocation which previous work had shown to be genetically linked to insecticide resistance. The exceptional clone had two other E4-encoding sites. The most resistant FE4-producing clone (800F) had amplified sequences at five sites (three loci: two homozygous and one heterozygous). Altogether, amplified E4 and/or FE4 sequences were found on four of the five autosome pairs of M. persicae. Possible origins of these multiple loci are discussed.
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Blackman, R L. 1974. Life cycle variation of Myzus persicae (Sulz.) (Horn., Aphididae) in different parts of the world, in relation to genotype and environment. Bull Ent Res, 63, 595–607.
Blackman, R L. 1987. Reproduction, cytogenetics and development. In: Minks, A. K. and Harrewijn, P. (eds) Aphids, Their Biology, Natural Enemies and Control, 2A, pp. 163–195. Elsevier, Amsterdam.
Blackman, R L. 1988. Rearing and handling aphids. In: Minks, A. K. and Harrewijn, P. (eds) Aphids, Their Biology, Natural Enemies and Control, 2B, pp. 59–68. Elsevier, Amsterdam.
Blackman, R L, and Devonshire, A L. 1978. Further studies on the genetics of the carboxylesterase regulatory system involved in resistance to organophosphorus insecticides in Myzus persicae (Sulzer). Pest Sci, 9, 517–521.
Blackman, R L, Devonshire, A L, and Sawicki, R M. 1977. Co- inheritance of increased carboxylesterase activity and resistance to organophosphorus insecticides in Myzus persicae (Sulzer). Pest Sci, 8, 163–166.
Blackman, R L, and Takada, H. 1977. The inheritance of natural chromosomal polymorphisms in the aphid Myzus persicae (Sulzer). Genetica, 47, 9–15.
Blackman, R L, Takada, H, and Kawakami, K. 1978. Chromosomal rearrangement involved in the insecticide resistance of Myzus persicae. Nature, 271, 450–452.
Devonshire, A L, and Field, L M. 1991. Gene amplification and insecticide resistance. Ann Rev Ent, 36, 1–23.
Devonshire, A L, Moores, G D, and Chiang, C. 1983. The biochemistry of insecticide resistance in the peach-potato aphid, Myzus persicae. Proceedings of the fifth International Congress of Pesticide Chemistry. Pest Chem, 3, 191–196.
Devonshire, A L, and Sawicki, R M. 1979. Insecticide-resistant Myzus persicae as an example of evolution by gene duplication. Nature, 280, 140–141.
Ferrari, J A, and Georghiou, G P. 1991. Quantitative genetic variation of esterase activity associated with a gene amplification in Culex quinquefasciatus. Heredity, 66, 265–272.
Field, L M, and Devonshire, A L. 1992. Esterase genes conferring insecticide resistance in aphids. In: Mullin, C. A. and Scott, J. G. (eds) Molecular Mechanisms of Insecticide Resistance Diversity Among Insects, pp. 209–217. American Chemical Society, Washington, DC.
Field, L M, Devonshire, A L, and Forde, B G. 1988. Molecular evidence that insecticide resistance in peach-potato aphids (Myzus persicae Sulz.) results from amplification of an esterase gene. Biochem J, 251, 309–312.
Field, L M, Javed, N, Stribley, M F, and Devonshire, A L. 1994. The peach-potato aphid, Myzus persicae and the tobacco aphid, M. nicotianae have the same esterase-based mechanism of insecticide resistance. Insect Mol Biol, 3, 143–148.
Field, L M, Williamson, M S, Moores, G D, and Devonshire, A L. 1993. Cloning and analysis of the esterase genes conferring insecticide resistance in the peach-potato aphid, Myzus persicae (Sulzer). Biochem J, 294, 569–574.
Heslop-Harrison, J S, Schwarzacher, T, Anamthawat-Jonsson, K, Leitch, A R, Shi, M, and Leitch, I J. 1991. In situ hybridization with automated chromosome denaturation. Tech J Meth Cell Mol Biol, 3, 109–116.
Levis, R W, Ganesan, R, Houtchens, K, Tolar, L A, and Sheen, F. 1993. Transposons in place of telomeric repeats at a Drosophila telomere. Cell, 75, 1083–1093.
Nance, E, Heyse, D, Britton-Davidian, J, and Pasteur, N. 1990. Chromosomal organization of the amplified esterase B1 gene in organophosphate-resistant Culex pipiens quinque-fasciatus Say (Diptera, Culicidae). Genome, 33, 148–152.
Pinkel, D, Straume, T, and Gray, J W. 1986. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA, 83, 2934–2938.
Raymond, M, Callaghan, A, Fort, P, and Pasteur, N. 1991. Worldwide migration of amplified insecticide resistance genes in mosquitoes. Nature, 350, 151–153.
Ruiz, J C, and Wahl, G M. 1990. Chromosomal destabilization during gene amplification. Mol Cell Biol, 10, 3056–3066.
Thompson-Stewart, D, Karpen, G H, and Spradling, A C. 1994. A transposable element can drive the concerted evolution of tandemly repetitious DNA. Proc Natl Acad Sci USA, 91, 9042–9046.
Trask, B J, and Hamlin, J L. 1989. Early dihydrofolate reductase gene amplification events in CHO cells usually occur on the same chromosome arm as the original locus. Genes Dev, 3, 1913–1925.
Wahl, G M. 1989. The importance of circular DNA in mammalian gene amplification. Cancer Res, 49, 1333–1340.
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Blackman, R., Spence, J., Field, L. et al. Chromosomal location of the amplified esterase genes conferring resistance to insecticides in Myzus persicae (Homoptera: Aphididae). Heredity 75, 297–302 (1995). https://doi.org/10.1038/hdy.1995.138
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DOI: https://doi.org/10.1038/hdy.1995.138
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