Abstract
Qualitative and quantitative studies show that diploid plants produced by anther culture (AC) from a double cross population of Brussels sprouts have arisen from spontaneously doubled haploid cells. The mean performance and distribution of lines produced by AC is compared to an equivalent array of inbred lines produced by single seed descent (SSD). There was no evidence of any difference in vigour between the AC and SSD samples. The tests are sufficiently sensitive to establish (with a 90 per cent confidence) that any true difference between the means, for vigour related traits, must be less than 15 per cent of the mean performance of SSD material. Additional evidence identifies genetic segregation for stability to micro-environmental factors within both the AC and SSD lines.
Similar content being viewed by others
Article PDF
References
Arcia, M A, Wernsman, E A, and Burk, L G. 1978. Performance of anther-derived dihaploids and their conventionally inbred parents as lines, in F1 hybrids and in F2 generations. Crop Sci, 18, 413–418.
Arus, P, and Orton, T J. 1983. Inheritance and linkage relationships of isoenzymes in Brassica oleracea. J Hered, 74, 405–412.
Brown, J S, and Wernsman, E A. 1982. Nature of reduced productivity of anther-derived dihaploid lines of flue-cured tobacco. Crop Sci, 22, 1–5.
Charmet, G, and Branlard, G. 1985. A comparison of androgenetic doubled-haploid and single seed descent lines in Triticale. Theor Appl Genet, 71, 193–200.
Jinks, J L, Chowdhury, M K U, and Pooni, H S. 1985. Comparison of the inbred lines derived from a hybrid of tobacco (burley × flue cured) by dihaploidy and single seed descent. Heredity, 55, 127–133.
Jinks, J L, and Pooni, H S. 1981. Properties of pure-breeding lines produced by dihaploidy, single seed descent and pedigree breeding. Heredity, 46, 391–395.
Jinks, J L, Pooni, H S, and Chowdhury, M K U. 1985. Detection of linkage and pleiotropy between characters of Nicotiana tabacum using inbred lines produced by dihaploidy and single seed descent. Heredity, 55, 327–333.
Levene, H. 1960. Robust test for equality of variances. In Olkin, I., Churye, S. G., Hoeffding, W., Madow, W. G. and Mann, H. G. (eds) Contributions to Probability and Statistics, Stanford University Press, Stanford, pp. 278–292.
Ockendon, D J. 1984. Anther culture in Brussels sprouts (Brassica oleracea var. gemmifera). I. Embryo yields and plant regeneration. Ann Appl Biol, 105, 285–291.
Riggs, T J, and Snape, E W. 1977. Effects of linkage and interaction in a comparison of theoretical populations derived by single seed descent and doubled haploid methods. Theor Appl Genet, 49, 111–115.
Rogers, W J, Kearsey, M J, and Smith, B M. 1987. The feasibility of producing inbred rather tha F1 hybrid cultivars in Brussels sprouts: Predictions from early generations. Ann Appl Biol, 111, 677–688.
Schnell, R J, Wensman, E A, and Burk, L G. 1980. Efficiency of single seed descent vs. anther-derived dihaploid breeding methods in tobacco. Crop Sci, 20, 619–622.
Scowcroft, W R, and Larkin, P J. 1983. Somaclonal variation and genetic improvement of crop plants. In Nugent, J. and O'Connor, M. (eds) Better Crops for Food, Ciba Foundation, London, pp. 173–197.
Setter, A P. 1988. Predicting the Results of Breeding Programmes in Outbreeding Species. Ph.D. Thesis, Faculty of Science, University of Birmingham.
Smith, B M, Rogers, W J, and Kearsey, M J. 1985. The feasibility of producing inbred rather than F1 hybrid cultivars in Brussels sprouts: a preliminary genetical analysis and choice of material for inbreeding. Ann Appl Biol, 107, 87–99.
Werner, C P, Setter, A P, Smith, B M, Kubba Jenan, and Kearsey, M J. Performance of recombinant inbred lines in Brussels sprouts (Brassica oleracea var. gemmifera). Theor Appl Genet (In press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kubba, J., Smith, B., Ockendon, D. et al. A comparison of anther culture derived material with single seed descent lines in Brussels sprouts (Brassica oleracea var. gemmifera). Heredity 63, 89–95 (1989). https://doi.org/10.1038/hdy.1989.79
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1989.79
This article is cited by
-
Comparison of single seed descent and anther culture-derived lines of three single crosses of rice
Theoretical and Applied Genetics (1993)
-
Molecular and morphological evaluation of doubled-haploid lines in maize. 2. Comparison with single-seed-descent lines
Theoretical and Applied Genetics (1993)