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Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery

Gene Therapy volume 17, pages 1384–1389 (2010)Cite this article

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Abstract

Adeno-associated viral (AAV) vectors, which are undergoing broad exploration in clinical trials, have significant promise for therapeutic gene delivery because of their safety and delivery efficiency. Gene delivery technologies capable of mediating localized gene expression may further enhance the potential of AAV in a variety of therapeutic applications by reducing spread outside a target region, which may thereby reduce off-target side effects. We have genetically engineered an AAV variant capable of binding to surfaces with high affinity through a hexa-histidine metal-binding interaction. This immobilized AAV vector system mediates high-efficiency delivery to cells that contact the surface and thus may have promise for localized gene delivery, which may aid numerous applications of AAV delivery to gene therapy.

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Acknowledgements

This work was supported by NIH R01HL081527. In addition, JJ was supported by a grant from the California Institute for Regenerative Medicine (Training Grant Number T1-00007). The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of CIRM or any other agency of the State of California.

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Authors and Affiliations

  1. Department of Chemical Engineering, The Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA

    J-H Jang, J T Koerber & D V Schaffer

  2. Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Korea

    J-H Jang

  3. Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY, USA

    K Gujraty, S R Bethi & R S Kane

  4. Department of Bioengineering, The Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA

    D V Schaffer

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  1. J-H Jang
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Correspondence to D V Schaffer.

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Jang, JH., Koerber, J., Gujraty, K. et al. Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery. Gene Ther 17, 1384–1389 (2010). https://doi.org/10.1038/gt.2010.81

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