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ICP10PK inhibits calpain-dependent release of apoptosis-inducing factor and programmed cell death in response to the toxin MPP+

Gene Therapy volume 15, pages 1397–1409 (2008)Cite this article

Abstract

Apoptosis is a widely accepted component of the pathogenesis of Parkinson's disease (PD), a debilitating neurodegenerative disorder characterized by loss of dopaminergic neurons in the substantia nigra. However, additional death programs were implicated, and current understanding of the cycle of intracellular events that leads to the demise of these neuronJis limited. Gene therapy strategies were proposed to inhibit apoptosis, but they have met with relatively limited success. Here we report that the antiapoptotic herpes simplex virus type 2 gene ICP10PK protects neuronally differentiated PC12 cells from death caused by 1-methyl-4-phenylpyridinium (in vitro PD model) through inhibition of calpain I activation and the resulting inhibition of Bax translocation to the mitochondria, apoptosis-inducing factor release and caspase-3 activation. Neuroprotection is through ICP10PK-mediated activation of the PI3-K/Akt survival pathway and upregulation/stabilization of the antiapoptotic protein Bcl-2 and the cytoprotective chaperone heat-shock protein 70.

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Author notes

  1. S Q Wales and J M Laing: These authors contributed equally to this work. These studies were supported by Public health service grant NS45169 and in part by the University of Maryland Statewide Health Network/other Tobacco-Related Diseases grant.

Authors and Affiliations

  1. Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, MD, USA

    S Q Wales, J M Laing, L Chen & L Aurelian

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Correspondence to L Aurelian.

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Wales, S., Laing, J., Chen, L. et al. ICP10PK inhibits calpain-dependent release of apoptosis-inducing factor and programmed cell death in response to the toxin MPP+. Gene Ther 15, 1397–1409 (2008). https://doi.org/10.1038/gt.2008.88

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