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A conserved sugar bridge connected to the WSXWS motif has an important role for transport of IL-21R to the plasma membrane

Genes & Immunity volume 16, pages 405–413 (2015)Cite this article

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Abstract

Interleukin-21 (IL-21) is a class I cytokine that belongs to the γc-subfamily of cytokines and regulates immune responses. It signals through a heterodimeric receptor complex composed of the IL-21R1 and γc-receptor chains. A characteristic feature of class I cytokine receptors is the presence of a consensus motif WSXWS (WS motif) in the membrane proximal fibronectin type III domain (FNIII) of these receptors. We recently described the structure of the IL-21R:IL-21 complex and showed that the first tryptophan of the WS motif of IL-21R is mannosylated and involved in formation of a sugar bridge that connects the two FNIII domains of the receptor. Furthermore, a mutation within the WS motif of IL-21R was recently shown to cause a novel kind of primary immunodeficiency syndrome (PID). Here, we report the structure of IL-21R alone, which shows that the sugar bridge forms independently of whether IL-21R binds IL-21 or not, and we furthermore investigate the role of this bridge in the export of IL-21R and γC to the plasma membrane. Thus, we provide a molecular explanation for how mutations in the WS motif may cause PIDs.

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Acknowledgements

We thank Siv A Hjorth and Kent Bondensgaard for help with purifying the IL-21R protein, as well as Lene Niemann Nejsum for advice on imaging. This work was supported by grants from the Danish Cancer Society and the Danish Council for Independent Research | Natural Sciences.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, University of Aarhus, Aarhus, Denmark

    P Siupka, O T Hamming, H H Gad & R Hartmann

  2. Novo Nordisk R&D Center China, Beijing, China

    L Kang

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Correspondence to R Hartmann.

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Siupka, P., Hamming, O., Kang, L. et al. A conserved sugar bridge connected to the WSXWS motif has an important role for transport of IL-21R to the plasma membrane. Genes Immun 16, 405–413 (2015). https://doi.org/10.1038/gene.2015.22

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