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Inflammation in vivo is modulated by GPR83 isoform-4 but not GPR83 isoform-1 expression in regulatory T cells

Genes & Immunity volume 11, pages 357–361 (2010)Cite this article

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Abstract

Most recently, we have described the G-protein coupled receptor 83 (GPR83), which is highly expressed by CD4+CD25+ regulatory T cells (Tregs) to be involved in the induction of CD4+Foxp3+ Tregs in the course of an ongoing immune response. Four GPR83 isoforms have been described. Here, we have shown that GPR83 isoform-4, which differs from GPR83 isoform-1 by 20 additional aminoacids in the second cytoplasmatic loop, is predominantly expressed by Tregs. Interestingly, GPR83 isoform-4 but not GPR83 isoform-1 retrovirally transduced T cells were able to interfere with inflammatory responses in vivo. Re-analysis of GPR83 transduced T cells revealed that this in vivo acquisition of suppressive activity was associated with the induction of Treg-associated molecules including Foxp3 in GPR83 isoform-4 but not GPR83 isoform-1 transduced CD4+ T cells under inflammatory conditions. Our results suggest that the 20 additional aminoacids within GPR83 isoform-4 are involved in Treg induction during inflammatory immune responses.

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Acknowledgements

We are very grateful to Carolin Wevers for excellent technical assistance and Lothar Groebe, Witold Bartosik and Annika Frede for cell sorting.

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Authors and Affiliations

  1. Institute of Medical Mircobiology, University Hospital Essen, Essen, Germany

    W Hansen, A M Westendorf, T Toepfer & J Buer

  2. Department of Veterinary Pathology, Free University, Berlin, Germany

    S Mauel & A D Gruber

  3. Array Facility, Helmholtz Centre for Infection Research, Braunschweig, Germany

    R Geffers

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  1. W Hansen
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  2. A M Westendorf
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  4. S Mauel
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  6. A D Gruber
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  7. J Buer
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Correspondence to W Hansen.

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Hansen, W., Westendorf, A., Toepfer, T. et al. Inflammation in vivo is modulated by GPR83 isoform-4 but not GPR83 isoform-1 expression in regulatory T cells. Genes Immun 11, 357–361 (2010). https://doi.org/10.1038/gene.2010.5

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