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The Sp1 transcription factor binds to the G-allele of the –1087 IL-10 gene polymorphism and enhances transcriptional activation

Genes & Immunity volume 10, pages 280–284 (2009)Cite this article

Abstract

The objectives of this study were to evaluate the influence of the −1087 single nucleotide polymorphism (SNP) on the gene expression of interleukin (IL)-10 and to identify transcription factors binding to this site in B cells. Using electrophoretic mobility-shift assays and nuclear extract from the DG75 B-cell line, we demonstrated that the Sp1 transcription factor bound to the −1087 G-allele of the IL-10 promoter and that the transcription factors PU.1 and Spi-B bound to both the G- and A-alleles. Transient transfections showed that lipopolysaccharide stimulation resulted in a 15-fold increase in promoter activity for the G-allele as compared to a 6-fold increase for the A-allele. Co-transfection with Sp1 expression vector in Sp1-deficient SL2 cells leading to Sp1 binding to the G-allele of the −1087 SNP resulted in increased IL-10 promoter activity. The results suggest a role for Sp1 transcription factor in the activation of IL-10 through the G-allele of the −1087 SNP in response to inflammatory signals.

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Acknowledgements

The current project was supported by grants from TUA Research, University of Gothenburg and Swedish Dental Society. We thank Dr Karen E Hedin (Department of Immunology, Mayo Clinic, Rochester, MN, USA) for providing the IL-10 GCC and ACC expression plasmids, G Suske (Klinikum der Philipps-Universität Marburg, Marburg, Germany) for the pPacO and the pPacSp1 expression plasmids and Cecilia Boreström (Department of Clinical Chemistry and Transfusion Medicine, University of Gothenburg) for assistance with the SL2 transfections.

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Authors and Affiliations

  1. Department of Periodontology, The Sahlgrenska Academy at University of Gothenburg, Göteborg, Sweden

    L Larsson, M Donati & T Berglundh

  2. Department of Clinical Chemistry and Transfusion Medicine, The Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, Göteborg, Sweden

    P Johansson, A Jansson & L Rymo

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  1. L Larsson
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Correspondence to T Berglundh.

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Larsson, L., Johansson, P., Jansson, A. et al. The Sp1 transcription factor binds to the G-allele of the –1087 IL-10 gene polymorphism and enhances transcriptional activation. Genes Immun 10, 280–284 (2009). https://doi.org/10.1038/gene.2008.79

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