Abstract
Purpose Congenital hypertrophy of the retinal pigment epithelium (CHRPE) exists almost exclusively among familial adenomatous polyposis (FAP) patients with adenomatous polyposis coli (APC) mutations between codon 413 in exon 9 and codon 1387 in exon 15. We investigated the locality of APC mutations in relationship to the occurrence of CHRPE in two Chinese families with FAP.
Methods Genomic DNA of available members of two unrelated Chinese FAP families was investigated for sequence alteration in the APC gene by polymerase chain reaction and direct sequencing, All subjects were examined by binocular indirect ophthalmoscopy (BIO) for CHRPE.
Results A mutation in exon 6, Arg216Stop, was identified in one patient with FAP and CHRPE. An Arg283Stop mutation in exon 8 was found in 5 members in another family; 4 of them had FAP and all had small hypopigmented white lesions, probably a new type of CHRPE.
Conclusions We found two mutations, Arg216Stop and Arg283Stop, upstream of codon 413 in FAP patients presenting with CHRPE. Arg283Stop has not previously been reported in such patients. A large-scale study on CHRPE and APC mutations in Chinese FAP patients is required to affirm their inter-relationships and the significance of the hypopigmented white lesions.
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Presented in part at the annual ARVO meeting, May 1997, Florida, USA
Proprietary interests: None Supported in part by: (1) a direct grant of the Medical Panel, the Chinese University of Hong Kong, (2) Mrs Annie Wong Eye Foundation, Hong Kong, and (3) Mr W.K. Lee Eye Foundation, Hong Kong
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Pang, C., Keung, J., Tang, N. et al. Congenital hypertrophy of the retinal pigment epithelium and APC mutations in two Chinese families with familial adenomatous polyposis. Eye 14, 18–22 (2000). https://doi.org/10.1038/eye.2000.5
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DOI: https://doi.org/10.1038/eye.2000.5