Abstract
A terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay was used to determine that apoptosis causes HeLa cell death induced by pseudolaric acid B. The c-Jun N-terminal kinase (JNK) inhibitor SP600125 decreased p53 protein expression during exposure to pseudolaric acid B. SP600125 decreased the phosphorylation of p53 during pseudolaric acid B exposure, indicating that JNK mediates phosphorylation of p53 during the response to pseudolaric acid B. SP600125 reversed pseudolaric acid B-induced down-regulation of phosphorylated extracellular signal-regulated protein kinase (ERK), and protein kinase C (PKC) was activated by pseudolaric acid B, whereas staurosporine, calphostin C, and H7 partly blocked this effect. These results indicate that p53 is partially regulated by JNK in pseudolaric acid B-induced HeLa cell death and that PKC participates in pseudolaric acid B-induced HeLa cell death.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Gong, X., Wang, M., Tashiro, Si. et al. Involvement of JNK-initiated p53 accumulation and phosphorylation of p53 in pseudolaric acid B induced cell death. Exp Mol Med 38, 428–434 (2006). https://doi.org/10.1038/emm.2006.50
Published:
Issue Date:
DOI: https://doi.org/10.1038/emm.2006.50
Keywords
This article is cited by
-
Roles and Interaction of the MAPK Signaling Cascade in Aβ25–35-Induced Neurotoxicity Using an Isolated Primary Hippocampal Cell Culture System
Cellular and Molecular Neurobiology (2021)
-
Activation of p53 contributes to pseudolaric acid B-induced senescence in human lung cancer cells in vitro
Acta Pharmacologica Sinica (2016)
-
Pseudolaric Acid B Circumvents Multidrug Resistance Phenotype in Human Gastric Cancer SGC7901/ADR Cells by Downregulating Cox-2 and P-gp Expression
Cell Biochemistry and Biophysics (2015)
-
Pseudolaric acid B inhibits inducible cyclooxygenase-2 expression via downregulation of the NF-κB pathway in HT-29 cells
Journal of Cancer Research and Clinical Oncology (2012)