Abstract
Two-site binding enzyme immunoassay for pregnancy-specific -β1 glycoprotein (SP1) has been developed using two monoclonal antibodies which can recognize all the three forms of SP1 from human sera. This assay system detects a minimal concentration of 1.25 ng/ml, and shows a linear dose-response curve at a range between 1.4 and 45.0 ng/ml. The recovery rates ranged from 90.0% to 113.3%, and intra-assay coefficients of variation were 15.8% and 8.7% for two different samples. When compared with a commercial EIA kit, this assay system had high correlation coefficient (r =0.92) but with somewhat lower values.
Similar content being viewed by others
Article PDF
Author information
Authors and Affiliations
Rights and permissions
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
About this article
Cite this article
Lee, JH., Kim, SR. & Chung, H. An enzyme immunoassay for pregnancy-specific β-1 glycoprotein (SP1), employing monoclonal antibodies. Exp Mol Med 28, 181–186 (1996). https://doi.org/10.1038/emm.1996.28
Published:
Issue Date:
DOI: https://doi.org/10.1038/emm.1996.28