Abstract
Fusion of cytotrophoblasts with the overlying syncytiotrophoblast is an integral step in differentiation of the human placental villous trophoblast. Multiple factors, such as growth factors, hormones, cytokines, protein kinases, transcription factors and structural membrane proteins, were described to modulate trophoblast fusion. However, the knowledge on remodelling of the membrane-associated cytoskeleton during trophoblast fusion is very limited. This study describes the link between remodelling of spectrin-like α-fodrin and intercellular trophoblast fusion. Experiments with primary trophoblasts isolated from term placentas and the choriocarcinoma cell line BeWo revealed a biphasic strategy of the cells to achieve reorganization of α-fodrin. Syncytialization of trophoblasts was accompanied by down-regulation of α-fodrin mRNA, whereas the full-length α-fodrin protein was cleaved into 120 and 150 kDa fragments. Application of calpeptin and calpain inhibitor III did not affect α-fodrin fragmentation in primary term trophoblasts and forskolin-treated BeWo cells, but decreased secretion of β human chorionic gonadotropin. In contrast, inhibitors of caspases 3, 8 and 9 attenuated generation of the 120 kDa fragment and a general caspase inhibitor completely blocked fragmentation, suggesting an exclusive function of caspases in α-fodrin remodelling. Immunofluorescence double staining of human placenta revealed co-localization of active caspase 8 with α-fodrin positive vesicles in fusing villous cytotrophoblasts. These results suggest that caspase-dependent fragmentation of α-fodrin may be important for reorganization of the sub-membranous cytoskeleton during trophoblast fusion.
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Main
Human placental villi are covered by the syncytiotrophoblast, a multinucleated layer devoid of lateral cell borders. This syncytial layer is in direct contact with maternal blood and constitutes the foeto-maternal blood barrier. Nuclei within the syncytiotrophoblast do not replicate1 and thus make the syncytium dependent on chromosomal DNA supplied from an exogenous source to counterbalance the continuous release of apoptotic nuclei into the maternal circulation. Acquisition of fresh nuclei is provided by the underlying cell layer of mononucleated cytotrophoblasts. Some of these villous cytotrophoblasts start to differentiate and consequently fuse with the overlying syncytiotrophoblast. This way not only nuclei, but also organelles and cytoplasm are transferred into the syncytium. Differentiation of villous cytotrophoblasts can be induced by growth factors, cytokines and hormones, which activate transcription of fusogenic genes, encoding proteins involved in trophoblast fusion.2
Fusion and conversion from the mononucleated to the syncytial state is fundamental for successful pregnancy, as the syncytiotrophoblast fulfils not only exchange of gas and nutrients between foetus and mother, but also performs endocrine functions. To meet these demands, the syncytiotrophoblast gains features originally missing in the villous cytotrophoblast compartment such as production of hormones like human chorionic gonadotropin (hCG), human placental lactogen3 or leptin,4 which are important for maintenance of pregnancy and adaptation of the maternal metabolism. However, not only hormones, also enzymes such as p450 aromatase5, 6 are solely expressed by the syncytiotrophoblast.
In addition to metabolic and endocrine adaptation, differentiation may also affect composition and arrangement of the cytoskeleton. At this point, the difference between a syncytium and a synkaryon should be noted, as the former arises from fusion of separate cells and the latter is formed by repeated karyokinesis without cytokinesis. Bearing this in mind, intercellular fusion comprises not only merger of cytoplasmic membranes and redistribution of cytoplasmic organelles, it also requires reorganization of the complete cytoskeleton.
The complex process of intercellular fusion includes at least three major stages. Initial steps such as (1) remodelling of the membrane architecture7 and (2) synthesis of proteins involved in membrane interaction8, 9, 10 are preceding the central step of (3) fusion pore formation. Finally, post-fusion steps such as fusion pore expansion11 and cytoplasmic reorganization take place.
This study focuses on the remodelling of spectrin-like α-fodrin (αII-spectrin, non-erythroid spectrin) during intercellular fusion of trophoblasts. Spectrins are composed of α and β heterodimers, which constitute the major component of the sub-membranous cytoskeleton of all cells. The spectrin network has a triangular structure, important for maintenance of cellular shape and membrane stability. Proteolytic cleavage of α-fodrin is implicated with apoptotic processes, as it is believed to be associated with membrane blebbing in apoptotic cells.12 Fragmentation of α-fodrin, an early event in apoptosis, is alternatively performed by calpain and caspase 3, which leads to generation of 150, 145 and 120 kDa cleavage products.13 However, α-fodrin cleavage may also proceed independent of calpain and caspase 3 activity, as shown during TGF-β-induced apoptosis of a murine B-cell line.14 In addition, fragmentation of α-fodrin occurs during differentiation of nerve cells15 and lens fibres16, 17 as well as during myoblast fusion.18 Here, we show localization of α-fodrin in human placental tissues and address the issue of spectrin remodelling during intercellular trophoblast fusion.
Results
Rearrangement of the α-fodrin network in human placenta and trophoblast cells
Immunofluorescence of human placental sections showed distinct staining of α-fodrin in mononucleated cytotrophoblasts, stromal cells and endothelial cells of stromal blood vessels. In first trimester (Figure 1a) and term placenta (not shown), α-fodrin was associated with apical and lateral cell borders of cytotrophoblasts, but was absent in the overlying syncytiotrophoblast.
A similar rearrangement of the α-fodrin network was observed in trophoblast cells when differentiation from the mononucleated to the syncytial state occurred. The trophoblast derived cell line BeWo showed pronounced α-fodrin staining as long as cells remained in an unstimulated, mononucleated state (Figure 1b). Forskolin triggers fusion of BeWo cells by increasing intracellular cAMP concentration through adenylyl cyclase activation.19 If BeWo cells were treated with forskolin cell borders and the associated α-fodrin network vanished in fusing cells (Figure 1c, asterisks), whereas the network remained intact in mononucleated cells (Figure 1c, arrowheads). This effect was also observed in isolated primary term trophoblasts, which spontaneously formed syncytia after 2 days of cultivation in vitro. Although α-fodrin lining was maintained in mononucleated cells (Figure 1d, arrowheads), it disappeared in syncytial areas (Figure 1d, asterisks). Forskolin treatment provoked a fusion rate of 36.0% (±1.3%) in BeWo cells, whereas primary term trophoblasts showed a fusion rate of 18.2% (±3.9%) after 48 h cultivation.
Down-regulation of α-fodrin mRNA in trophoblast cells during syncytialization
Down-regulation of α-fodrin mRNA expression was pronounced in BeWo cells treated with forskolin. Stimulation with the fusion inducing reagent led to an expression level of only 37% compared with vehicle-treated cells after 48 h (Figure 2a). In primary term trophoblasts, α-fodrin mRNA expression was also down-regulated in a time-dependent way and declined after 72 h of culture to 52% of the expression level at cultivation start (Figure 2b). This effect was also observed in microarray analysis, which revealed a down-regulation of both α-fodrin (2.19-fold) and β-fodrin (2.21-fold) in forskolin-treated BeWo cells after cultivation for 72 h.
Oxygen regulates α-fodrin mRNA in BeWo cells
Earlier studies revealed an integral function of oxygen tension in modulating trophoblast syncytialization, as cultivation under low oxygen impaired intercellular fusion of primary trophoblasts and BeWo cells.20, 21, 22 To address whether oxygen tension also affects α-fodrin expression, BeWo cells were cultured under a broad range of oxygen concentrations, including 2% (placental normoxia in first trimester), 8% (placental normoxia at term), 16% (placental hyperoxia was concluded in pregnancy disorders23) and 20% (normal cell culture value) oxygen. Cultivation of unstimulated BeWo cells under these conditions for 48 h indeed affected the expression level of α-fodrin, as rising oxygen concentrations impaired mRNA expression of α-fodrin: when oxygen was set to 16%, α-fodrin expression decreased to 76.5% (±2.4%, P<0.005) of the expression level at 2% oxygen (Figure 2c). Similar was the effect at 20% oxygen and here α-fodrin expression declined to 78.8% (±5.7%, P<0.05) compared with the expression at 2% oxygen (Figure 2c). In contrast, expression of hCG beta subunit mRNA (Figure 2d) as well as secreted βhCG protein (not shown) corresponded with oxygen concentrations, as it was up-regulated with rising oxygen concentrations. Thus, low oxygen levels may well be responsible for impaired trophoblast fusion as expression of βhCG mRNA was 2.4- and 3.7-fold higher at 8 and 20% oxygen, respectively, compared with expression at 2% oxygen (Figure 2d).
Fragmentation of α-fodrin protein during trophoblast fusion
Forskolin stimulation of BeWo cells for 48 h led to augmented α-fodrin fragmentation as observed by generation of truncation products of approximately 150 and 120 kDa (Figure 3a). In BeWo cells, fragmentation of α-fodrin proceeded in a time- and forskolin-dependent manner, whereas simultaneous incubation with DMSO alone caused only weak fragmentation. Interestingly, forskolin treatment provoked only low α-fodrin cleavage in Jeg-3 cells and had no effect in JAR cells, both choriocarcinoma cell lines that do not show syncytial fusion (not shown). In BeWo cells, forskolin-induced α-fodrin fragmentation and syncytialization was associated with a time-dependent increase in βhCG secretion (Figure 3b). In primary term trophoblasts, α-fodrin was also fragmented during culture, albeit fragmentation proceeded slower (Figure 3c). Immunoblots of primary term trophoblasts and forskolin-challenged BeWo cell lysates revealed that α-fodrin was cleaved into 150 and 120 kDa fragments. However, it has been shown that α-fodrin is cleaved into 150, 145 and 120 kDa break down products in apoptotic cells.24 Thus, the question arose whether the 145 kDa fragment was not detected because of insufficient protein separation by electrophoresis or whether it was even not generated in fusing trophoblasts. To solve this problem, the α-fodrin fragmentation pattern of forskolin-treated BeWo cells was compared with that of human umbilical vein endothelial cells (HUVEC), treated with calcium ionophores such as ionomycin and A23187 (Figure 3d). Both, ionomycin and A23187 clearly induced the generation of a detectable 150/145 kDa doublet only in HUVEC. These experiments excluded the possibility of insufficient protein separation and confirmed that no 145 kDa fragment is generated in fusing BeWo cells.
According to literature, α-fodrin is fragmented by the combined action of calpain and caspase 3.25 To test whether or not these proteases were involved in α-fodrin fragmentation in primary term trophoblasts and forskolin-treated BeWo cells, specific inhibitor peptides were applied to the cells. Calpeptin and calpain inhibitor III, both cell permeable calpain inhibitors, had no effect on α-fodrin fragmentation, regardless of testing in forskolin-stimulated BeWo cells (Figure 4a) or primary trophoblasts (Figure 4b). To confirm that calpeptin and calpain inhibitor III were working correctly and specifically, BeWo and HUVEC cells were treated with ionomycin in the presence or absence of both calpain inhibitors. Although ionomycin did not induce α-fodrin fragmentation into a 150/145 kDa doublet in BeWo cells, it led to massive generation of both fragments in HUVEC (Figure 4c). In the presence of calpeptin or calpain inhibitor III, ionomycin-induced α-fodrin fragmentation was almost completely blocked in HUVEC, confirming the efficiency of the applied inhibitors.
If inhibitors of caspase 8 or 3 (IETD or DEVD, respectively) were added to the culture medium, generation of the 120 kDa fragment was diminished in forskolin-treated BeWo cells as well as primary term trophoblasts (Figure 4a and b). In both cell types, impaired generation of the 120 kDa fragment was accompanied by an increase of the 150 kDa cleavage product (Figure 4a and b). Besides caspases 8 and 3 inhibitors, also a caspase 9 inhibitor (LEHD) was able to inhibit the generation of the 120 kDa fragment, but failed to inhibit the 150 kDa fragment in forskolin-treated BeWo cells (Figure 4a) and primary term trophoblasts (Figure 4b). However, if the general caspase inhibitor Q-VD-OPh was present in the culture medium, α-fodrin cleavage was almost entirely blocked in primary term trophoblasts (Figure 4b) and completely inhibited fragmentation in forskolin-treated BeWo cells (Figure 4a). Combined administration of calpain and caspase inhibitors did not alter the fragmentation patterns observed in experiments with individual application of caspase inhibitors (not shown).
However, caspase inhibitor experiments only indirectly showed caspase activation in fusing trophoblasts. Thus, western blots were performed to prove activation of caspases 3, 8 and 9 in forskolin-treated BeWo cells. Forskolin treatment slightly increased cleavage products of caspases 3, 8 and 9, compared with vehicle control after 48 h (Figure 4d), whereas 2 h incubation with staurosporine led to pronounced activation of caspases 3, 8 and 9. Ionomycin had only marginal effects on caspase activation in BeWo cells (Figure 4d).
βhCG secretion from trophoblasts was impaired by inhibitors of calpains and caspases
In BeWo cells, βhCG secretion increased during 48 h forskolin treatment 9.2-fold (P<0.001), whereas calpeptin and calpain inhibitor III reduced forskolin-mediated βhCG secretion to 47.2% (±0.3%, P<0.001) and 61.4% (±1.1%, P<0.001), respectively (Figure 5a). Inhibitors of caspases 8 (IETD), 3 (DEVD) and 9 (LEHD) impaired βhCG secretion from forskolin-treated BeWo cells to 67.1% (±2.6%, P<0.001), 74.0% (±1.6%, P<0.001) and 76.2% (±1.3%, P<0.001), respectively, compared with controls. Besides individual caspase inhibitors, the general caspase inhibitor Q-VD-OPh reduced βhCG secretion in forskolin-treated BeWo cells to 66.5% (±1.3%, P<0.001; Figure 5a).
Cultivation of primary term trophoblasts in the presence of calpeptin and calpain inhibitor III impaired secretion of βhCG to 27.6% (±6.3%, P<0.001) and 41.0% (±7.3%, P<0.001) after 48 h compared with conditions without inhibitors (Figure 5b). Inhibitors of caspases 8 (IETD), 3 (DEVD) and 9 (LEHD) led to a reduction in βhCG secretion to 76.6% (±6.6%, P=0.014), 78.8% (±5.8%, P=0.018) and 83.0% (±5.5%, P<0.05), respectively, compared with controls (Figure 5b). The general caspase inhibitor Q-VD-OPh decreased βhCG secretion in primary term trophoblasts to 77.7% (±1.2%, P<0.01).
Effect of calpain and caspase inhibitors on BeWo cell fusion
Forskolin stimulation of BeWo cells for 48 h enhanced syncytialization, as determined by the percentage of nuclei in syncytia, which increased from 5.8% (±0.5%) to 36.0% (±1.3%, P<0.001) (Figure 5c). Application of calpain inhibitors (calpeptin and calpain inhibitor III) or inhibitors of caspases 3 (DEVD) and 9 (LEHD) did not significantly affect the fusion rate of forskolin-treated BeWo cells after 48 h (35.3±1.2%, 34.9±1.3%, 35.5±1.2% and 36.3±1.0%, respectively). However, if caspase 8 inhibitor (IETD) or the general caspase inhibitor (Q-VD-OPh) were added to BeWo cell cultures, fusion rates of forskolin-treated BeWo cells decreased to 30.9% (±1.2%, P<0.001) and 30.7% (±1.0%, P<0.001), respectively (Figure 5c).
Active caspase 8 in villous trophoblasts of human placenta
Double staining of human first trimester placenta with anti-α-fodrin and anti-cleaved-caspase-8 antibodies showed active caspase 8 in few villous cytotrophoblasts (Figure 6a) as well as in restricted areas of the syncytiotrophoblast (Figure 6g). We have recently showed that one out of 422 villous cytotrophoblasts and one out of 759 nuclei within the syncytium had a perinuclear staining for active caspase 8.26 Active caspase 8 was also observed in cytotrophoblasts, which detached from the basement membrane and obviously left the cell layer of mononucleated cytotrophoblasts towards the overlying syncytiotrophoblast (Figure 6d). In these cells, α-fodrin, in particular after transition into the syncytium, appeared decomposed in vesicles as shown in the red channel (Figure 6b, e and h). Staining of cleaved caspase 8 was less intense in the syncytiotrophoblast compared with cytotrophoblasts positive for cleaved caspase 8 (Figure 6c, i).
Discussion
The data presented in this study provide evidence that villous cytotrophoblasts pursue two strategies to achieve remodelling of α-fodrin during syncytialization. One route is down-regulation of α-fodrin mRNA, a mechanism likely to take place in the light of the loss of lateral cell borders and reduction of total membrane area in the syncytiotrophoblast. This finding is in line with down-regulation of other structural proteins such as E-cadherin27 and desmoplakin,28 which are no longer needed after syncytialization.
The second route comprises proteolytic fragmentation of already existing α-fodrin, which lines the inner membrane leaflet in villous cytotrophoblasts. Cleavage of α-fodrin is implicated to affect cell shape and membrane morphology, as shown for platelet activation, which includes cell shape transformation from discs into irregular spheres.29 As degradation of the sub-membranous cytoskeleton network affects the membrane curvature, it is tempting to speculate that spatial fragmentation of the fodrin meshwork is required for fusion with adjacent cell membranes. This assumption is substantiated by the fact that the more curved a membrane is, the more fusogenic it becomes.30 For villous trophoblast, this means that a local disruption of the fodrin network, in combination with up-regulation of fusogenic membrane proteins, such as the endogenous viral envelope protein syncytin 1, would initiate membrane fusion with the overlying syncytiotrophoblast membrane.
Proteolytic breakdown of α-fodrin into 150, 145 and 120 kDa fragments has been shown to be mediated alternatively by calpain and caspase 3, depending on cell type and pathway of activation.13 However, in fusing trophoblasts α-fodrin is only cleaved into 150 and 120 kDa fragments, which may arise in a two-phase reaction, with an initial cleavage generating the 150 kDa fragment and a second phase of producing a 120 kDa fragment.31 Application of specific, cell permeable peptide inhibitors of calpain and caspases provides strong evidence for an exclusive action of caspases, but not that of calpains in cleavage of α-fodrin during trophoblast differentiation. Initial activation of pro-caspase 8 may lead to activation of caspases 9 and 3, which subsequently results in α-fodrin fragmentation.
Cleaved pro-caspase 8 can indeed be detected in a small subset of villous cytotrophoblasts as well as in cytotrophoblasts leaving the basement membrane towards the syncytiotrophoblast (Figure 6). Interestingly, staining of cleaved pro-caspase 8 co-localizes with α-fodrin positive cytoplasmic vesicles, suggesting α-fodrin degradation in those areas. The involvement of caspase 8 in trophoblast fusion is consistent with earlier findings, showing an inhibitory effect of caspase 8 antisense oligonucleotides or inhibitor peptides on fusion of cytotrophoblast with the syncytiotrophoblast in a human villous explant model.32
Here, we show a marginal effect of the caspase 8 inhibitor on BeWo cell fusion, whereas a much stronger effect of caspase 8 inhibitor was shown on trophoblast fusion in the placental villous explant model.32 A comparison between two completely different in vitro models is always hard to draw. Interpretation of such data should always be carried out bearing in mind the cell type and microenvironment used for the study. Furthermore, it needs to be taken into account that BeWo cells are derived from a choriocarcinoma and thus most probably have alterations in their apoptosis programme. Hence, BeWo cells may have established other means than the use of caspase 8 to successfully fuse with other cells. In any event, in both systems only inhibition of caspase 8 and not of calpains or caspase 3 or 9 led to a significant reduction in the rate of syncytial fusion.
Beside cleavage of α-fodrin, active caspase 8 may also mediate externalization of phosphatidylserine to the outer leaflet,33, 34 a process required in myotube formation,35 sperm–egg fusion36 and trophoblast syncytialization.37 Caspase 8 acts upstream of caspase 3, but may additionally activate several other pathways during trophoblast fusion. This may explain why administration of caspase 3 inhibitor alone did not affect syncytialization of forskolin-treated BeWo cells.
Calpains are not involved in α-fodrin cleavage during trophoblast fusion, as two different inhibitors of calpains did not affect fragmentation. Secretion of βhCG, however, is diminished in the presence of the applied calpain inhibitors. How calpains interfere with secretion of hCG, as observed after application of calpain inhibitors, remains to be elucidated.
As inhibitors of caspases 3, 8 and 9 did not inhibit the generation of the 150 kDa fragment of α-fodrin during trophoblast fusion, the initial cleavage may be executed by other proteases. The general caspase inhibitor Q-VD-OPh completely blocked α-fodrin cleavage in forskolin-challenged BeWo cells, which argues for the involvement of other caspases. Potential candidates are caspases 1, 2, 4, 6 and 7, which are all able to generate the 150 kDa fragment, presumably because of cleavage at a proteolytically hypersensitive site within α-fodrin.24 This hypersensitive site is located near the junction repeat units 10–11 almost in the centre of α-fodrin and is highly accessible to enzymatic cleavage.24, 38 Recent in vitro experiments provide evidence that caspases 2 and 7 target the same cleavage site (at Asp-1185) as caspase 3,39 suggesting that in trophoblast fusion the 150 kDa fragment of α-fodrin may be produced by one of these caspases. Administration of caspases 3 and 8 inhibitors only blocks the generation of the 120 kDa fragment, leading to an accumulation of the 150 kDa product. Similar effects were described earlier by Brown et al.,14 who clearly showed that BD-fmk, a broad spectrum caspase inhibitor, was not able to inhibit the generation of the 150 kDa fragment in a TGF-β-treated B-cell line. According to their study, generation of the 150 kDa fragment arose by the action of different proteases and was rather not caspase specific. However, comparison of both studies is difficult as cells and types of stimuli were different.
Intercellular fusion of forskolin-treated BeWo cells is only marginally affected by the general caspase inhibitor Q-VD-OPh, even though α-fodrin fragmentation is completely blocked in the presence of the inhibitor. Hence, fragmentation of α-fodrin may rather proceed right after the fusion process. This assumption is supported by immunofluorescence of human first trimester placenta, showing α-fodrin decomposition in cytotrophoblasts, which have left the basal membrane towards the overlying syncytiotrophoblast. However, caspases, in particular caspase 8, may also activate pathways before fusion, as forskolin-induced syncytialization of BeWo cells is impaired not only by the general caspase inhibitor, but also by the individual inhibitor of caspase 8.
There is a growing body of evidence suggesting that caspases are not only involved in apoptosis, but also in other cellular processes such as cell-cycle regulation40 and differentiation.41 Increasing secretion of hCG from primary trophoblasts and forskolin-stimulated BeWo cells argues for differentiation into the syncytial state, rather than apoptosis to proceed. This resembles the in vivo situation, as the syncytiotrophoblast and not the villous cytotrophoblast expresses hCG.42 Caspase and calpain activities as well as cleavage of the sub-membranous fodrin cytoskeletal network seem to have their specific functions in trophoblast fusion.
Materials and Methods
Human placenta tissue samples
The study was approved by the ethical committee of the Medical University of Graz. Informed consent was obtained from the patients. Six first trimester placentas between weeks 6 and 12 of gestation were obtained from pregnancy terminations for psychosocial reasons. Three term placentas were obtained from uncomplicated pregnancies after delivery between weeks 38 and 40. Tissue samples from first trimester and term placentas were fixed and paraffin embedded using the Hepes Glutamic Acid Buffer Mediated Organic Solvent Protection Effect (HOPE; Innovative Diagnostik Systeme, Hamburg, Germany) fixation technique, as described earlier.43
Culture of BeWo cells, primary term trophoblasts and HUVEC
BeWo cells were purchased from the European Collection of Cell Cultures and cultured according to the supplier's instructions. In brief, cells were cultured in a 1 : 1 mixture of DMEM (Gibco, Invitrogen, Paisley, UK) and Ham's F12K (Gibco), supplemented with 10% FCS, penicillin/streptomycin, amphotericin B, L-glutamine and non-essential amino acids. Cells between passage 10 and 20 were used for in vitro experiments. Syncytialization of BeWo cells was induced with forskolin.19 Cell culture medium was supplemented with forskolin (Sigma, St Louis, MO, USA) at a final concentration of 20 μM (10 mM stock in DMSO). Control cells were incubated with culture medium containing the same volume of DMSO (0.2%).
Mononucleated term trophoblasts were isolated from chorionic villi by enzymatic digestion and Percoll density gradient centrifugation as described earlier.44 Primary term trophoblasts were cultured in DMEM (Gibco) supplemented with 10% FCS in a humidified atmosphere of 5% CO2 at 37°C.
HUVEC were purchased from PromoCell (Heidelberg, Germany) and cultured in EBM Endothelial Basal Medium (Lonza, Walkersville, MD, USA) including the supplement kit EGM-MV SingleQuots (Lonza).
Culture of BeWo cells at various oxygen concentrations
BeWo cells were plated in six-well dishes (4 × 105 cells/well) in DMEM/Ham's F12K (2 ml/well) and cultured in a hypoxic workstation (BioSpherix, Redfield, NY, USA) under 2, 8, 16 and 20% oxygen for 48 h. Cell culture supernatants, total protein and RNA were collected at indicated oxygen concentrations directly in the workstation. Experiments were performed in triplicates using three independent preparations.
Caspase and calpain inhibitor experiments
BeWo cells (2 × 105 cells/well) were plated in 12-well dishes in their appropriate culture medium 1 day before start of experiments. On the next day, cells were preincubated for 30 min with calpeptin (Z-Leu-NLe-CHO, Calbiochem, Darmstadt, Germany), calpain inhibitor III (Z-Val-Phe-CHO, Calbiochem), caspase 3 inhibitor peptide (Z-DEVD-FMK, BD PharMingen, Franklin Lakes, NJ, USA), caspase 8 inhibitor peptide (Z-IETD-FMK, BD PharMingen), caspase 9 inhibitor peptide (Z-LEHD-FMK, R&D Systems, Minneapolis, MN, USA), general caspase inhibitor (Q-VD-OPh, R&D Systems) or negative control peptide (Z-FA-FMK, BD PharMingen) at a concentration of 20 μM each. Control cells were incubated with the same volume of DMSO, as all inhibitors were solubilized in this solvent. After preincubation, cells were incubated with medium containing the respective inhibitor together with either forskolin (20 μM) or DMSO (0.2%). At the same time, cells were also incubated only with forskolin or DMSO without inhibitors. After 48 h incubation, cells were lysed and lysates were subjected to immunoblot analyses.
Primary term trophoblasts (2 × 105 cells/well) were plated in 12-well dishes and cultivated in DMEM/10% FCS with or without the respective inhibitors (20 μM) for 48 h. After incubation, cells were lysed and lysates were subjected to western blot analysis. Inhibitor experiments with BeWo cells and primary term trophoblasts were repeated three times using different cell preparations.
Treatment of BeWo cells and HUVEC with calcium ionophores and calpain inhibitors
BeWo cells (4 × 105 cells/well) and HUVEC (2.8 × 105 cells/well) were plated in six-well dishes in their appropriate culture medium 1 day before start of experiments. Next day, cells were preincubated for 30 min with calpeptin (20 μM), calpain inhibitor III (20 μM) or vehicle control (DMSO, 0.2%). After preincubation, cells were treated with the calcium ionophores A23187 (10 μM, 2 h; Sigma-Aldrich, St Louis, MO, USA) or ionomycin (10 μM, 1.5 h; Sigma-Aldrich) in the presence or absence of the respective inhibitors. Experiments were repeated three times with different cell passages.
Measurement of secreted βhCG
Culture medium of the cells was collected at indicated time points and centrifuged at 4000 r.p.m. for 10 min. Supernatants were stored at −20°C and subjected in groups to immunoassay analyses (Dimension Xpand; Dade Behring Inc., Deerfield, IL, USA). Values of secreted βhCG were normalized to total protein in cell lysates.
Immunofluorescence staining
BeWo cells (8 × 104) were plated in chamber-slides (Nunc, Rochester, NY, USA) 1 day before start of experiments. On the next day, medium was replaced by medium containing forskolin (20 μM) or DMSO (0.2%) and cells were cultivated for 48 h. After incubation, cells were washed with PBS, dried and fixed for 10 min in acetone. Fixation was followed by rehydration with PBS and subsequent incubation with UV Block (LabVision, Fremont, CA, USA) for 7 min. Primary antibody, anti-α-fodrin (clone AA6, BioTrend, Kőln, Germany), was diluted 1 : 100 in antibody diluent (DAKO, Carpintera, CA, USA) and applied to the slides for 30 min. Slides were washed with PBS and incubated with secondary antibody, Alexa Fluor 555 goat anti-mouse (1 : 200; Invitrogen, Eugene, OR, USA) for 30 min. Finally, slides were washed and nuclei were stained with DAPI (1 : 2000; Invitrogen) for 5 min.
HOPE fixed, paraffin-embedded human first trimester placental tissues43 were cut in 5 μm sections and mounted on Superfrost Plus slides (Menzel-Glaeser, Braunschweig, Germany). Sections were deparaffinized according to standard procedure. Slides were incubated with the primary antibody, anti-α-fodrin (1 : 100), for 30 min. Subsequently, slides were washed and incubated with Alexa Fluor 555 goat anti-mouse (1 : 200; Invitrogen) for 30 min. Slides were washed and nuclei were stained with DAPI (1 : 2000; Invitrogen). For double staining of α-fodrin and cleaved caspase 8, slides were boiled in 10 mM sodium citrate buffer (pH 6.0). Monoclonal mouse anti-α-fodrin and monoclonal rabbit anti-cleaved-caspase-8 antibody (clone 18C8, Cell Signaling, Beverly, MA, USA) were mixed and diluted in antibody diluent (both 1 : 100). Primary antibodies were incubated on the sections at 4°C over night. Slides were washed and incubated with a mixture of Alexa Fluor 555 goat anti-mouse and Alexa Fluor 488 goat anti-rabbit antibodies (both 1 : 200, Invitrogen) for 30 min at room temperature. For negative controls, rabbit immunoglobulin fraction (DAKO) or negative control mouse IgG1 (DAKO) were diluted in antibody diluent (1 μg/ml) and incubated on slides as described above. Incubation with negative control immunoglobulins revealed no staining. After staining procedure, cells as well as tissue sections were mounted with ProLong Gold antifade reagent (Invitrogen). Fluorescence microscopy was performed using an Axiophot and an AxioCam HRc (Zeiss, Oberkochen, Germany).
Assessment of syncytialization
BeWo cells were treated as described in the immunofluorescence section and stained according to the protocol. Instead of the anti-α-fodrin antibody, a monoclonal anti-E-cadherin antibody (1 : 15 diluted; Acris GmbH, Hiddenhausen, Germany) was applied to visualize cell–cell contacts. For unbiased quanitification of BeWo cell syncytialization, a microscope (Leica, Wetzlar, Germany; DM6000B), equipped with a motorized stage, a digital camera (Olympus, Hamburg, Germany; DP72) and the newCAST stereology software (Visiopharm) was used. The system systematically randomly selected and microphotographed 20 positions per chamber ( × 20 objective). Counting frames, which covered 80% of every single image, were applied to the images and nuclei in syncytia were counted and related to the total number of nuclei in the selected areas. All fusion experiments were conducted in triplicate and repeated thrice with different BeWo cell passages.
Immunoblotting
Cells were washed with PBS and lysed in lysis buffer (10 mM Tris pH 7, 1% SDS, 1 mM sodium-orthovanadate and a protease inhibitor cocktail). Protein concentration was determined by the Lowry technique and for α-fodrin immunodetection, 30 μg total protein were applied to precast 3–8% Tris–acetate gels (NuPAGE, Novex; Invitrogen). After electrophoresis, proteins were semi-dry blotted on a 0.45 μm nitrocellulose membrane (Hybond, Amersham Biosciences, Little Chalfont, UK). Blotting efficiency was determined by staining the nylon membranes with Ponceau S solution (Sigma-Aldrich). Immunodetection was conducted with a chemiluminescent immunodetection kit (Western Breeze; Invitrogen) according to the manufacturers’ instructions. Monoclonal anti-α-fodrin (clone AA6, BioTrend, Köln, Germany) was diluted in blocking solution 1 : 2000 and applied to the membrane for 1 h at RT.
For detection of cleaved caspases, 30 μg total protein of forskolin (20 μM, 48 h)- or DMSO (0.2%, 48 h)-treated BeWo cells were separated on 4–12% Bis–Tris gels (NuPAGE, Invitrogen). For controls, lysates were used from BeWo cells treated with staurosporine (2 μM, 2 h; Alexis Biochemicals, Lausen, Switzerland) or ionomycin (10 μM, 1.5 h). Blotting and chemiluminescent immunodetection were performed as described above. Primary antibodies, monoclonal anti-cleaved caspase 3 (clone 5A1E), monoclonal anti-cleaved caspase 8 (clone 18C8) and polyclonal anti-cleaved caspase 9 (all Cell Signaling), were diluted 1 : 1000 and incubated with the membrane over night at 4°C.
Microarray analyses
Applied Biosystems Human Genome Survey Arrays were used to determine the transcriptional profiles of BeWo cells treated with forskolin (20 μM, 48 h) or DMSO (0.2%, 48 h). DIG-labelled cDNA probes were generated by reverse transcription of 20 μg total RNA using the Chemiluminescent RT-Labeling kit (Applied Biosystems, Foster City, CA, USA) as described by the manufacturer's protocols. Array hybridization, chemiluminescence detection, image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit and Applied Biosystems 1700 Chemiluminescence Microarray Analyzer following the manufacturers’ instructions.
Each microarray was first pre-hybridized at 55°C for 1 h in hybridization buffer with blocking reagent. Oligo-dT-primed, DIG-labelled cDNA targets were fragmented, mixed with internal control target and then hybridized to the equilibrated microarrays in a volume of 1.5 ml at 55°C for 16 h. After hybridization, the arrays were washed with hybridization wash buffer and chemiluminescence rinse buffer. Enhanced chemiluminescent signals were generated by incubating arrays with alkaline phosphatase-conjugated anti-digoxigenin antibody followed by incubation with Chemiluminescence Enhancing Solution and a final addition of Chemiluminescence Substrate. Four images were collected for each microarray using the ABI 1700 Chemiluminescent Microarray Analyzer. Images were auto-gridded and the chemiluminescence signals were quantified, corrected for background and spot and spatially normalized.
Real-time RT-PCR analyses
Total RNA was isolated with the Tri Reagent (Molecular Research Center, Cintinnati, OH, USA) according to the manufacturers’ instructions. The quality of BeWo total RNA (RIN 9.0–9.6) was assessed with a bioanalyzer (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA). Reverse transcription of total RNA was performed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA) according to manufacturer's manual. In brief, 2 μg total RNA of each sample was mixed with the kit components in a total reaction volume of 20 μl and incubated for 10 min at 25°C, 120 min at 37°C and 5 s at 85°C in a thermocycler.
Real-time PCR was performed using the QuantiFast SYBR Green PCR Kit (Qiagen, Hilden, Germany) and QuantiTect Primer Assays (Qiagen) for alpha-fodrin (Hs_SPTAN1_1_SG QuantiTect Primer Assay) and ribosomal protein P0 (RPLP0, Hs_RPLP0_1_ SG QuantiTect Primer Assay), used as internal reference. A total of 8.8 μl of cDNA (10 ng/μl) were mixed with 2.2 μl primers and 11 μl SYBR green mastermix. Expression levels were analysed in triplicates in a volume of 6 μl per well in a 384-well plate (Roche, Mannheim, Germany) in a LightCycler 480 (Roche). PCR conditions contained an initial 5 min denaturing step at 95°C and a subsequent two-step cycling including 10 s at 95°C and 30 s at 60°C for 40 cycles. Ct values were automatically generated by the LightCycler 480 Software (Roche) and relative quantification of gene expression was calculated by standard ΔΔCt method using the expression of RPLP0 as reference.
Statistical analysis
Data are presented as mean±S.D. and were tested using Student's t-test for differences between groups using SigmaPlot 11.0 and MS Excel 2003. Significances were accepted at a level P<0.05.
Abbreviations
- cAMP:
-
cyclic adenosine monophosphate
- βhCG:
-
human chorionic gonadotropin beta subunit
- DMSO:
-
dimethyl sulfoxide
- hPL:
-
human placental lactogen
- HUVEC:
-
human umbilical vein endothelial cells
- TGF-β:
-
transforming growth factor-beta
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Acknowledgements
This work was supported by grants from the EU (Grant # 037244, project title Pregenesys) and the Franz-Lanyar-Foundation (Projects # 321 and # 331).
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Gauster, M., Siwetz, M., Orendi, K. et al. Caspases rather than calpains mediate remodelling of the fodrin skeleton during human placental trophoblast fusion. Cell Death Differ 17, 336–345 (2010). https://doi.org/10.1038/cdd.2009.133
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DOI: https://doi.org/10.1038/cdd.2009.133
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