Abstract
The one-bead-one-compound (OBOC) combinatorial peptide library is a powerful tool to identify ligand and receptor interactions. Here, we applied the OBOC library technology to identify mimotopes specific to the immunoglobulin E (IgE) epitopes of the major shellfish allergen tropomyosin. OBOC peptide libraries with 8–12 amino acid residues were screened with serum samples from patients with shellfish allergy for IgE mimotopes of tropomyosin. Twenty-five mimotopes were identified from the screening and their binding reactivity to tropomyosin-specific IgE was confirmed by peptide ELISA. These mimotopes could be divided into seven clusters based on sequence homology, and epitope mapping by EpiSearch of the clustered mimotopes was performed to characterize and confirm the validity of mimotopes. Five out of six of the predicted epitopes were found to overlap with previously identified epitopes of tropomyosin. To further confirm the mimicry potential of mimotopes, BALB/c mice were immunized with mimotopes conjugated to keyhole limpet hemocyanin and assayed for their capacity to induce tropomyosin-specific antibodies. BALB/c mice that received mimotope immunization were found to have an elevated level of tropomyosin-specific immunoglobulin G, but not mice that received an irrelevant mimotope. This study pioneers the successful application of the OBOC libraries using whole sera to screen and identify multiple shrimp allergen mimotopes and validates their mimicry potential using in vitro, in vivo, and in silico methods.
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Acknowledgements
The authors thank K.C. Cheung, Y.F. Lam, T.S. Cheung, and W.H. Wong (CUHK) for their technical assistance and Dr. David Wilmshurst (CUHK) for editing the manuscript. The authors thank the Combinatorial Chemistry Shared Resource at the University of California, Davis for assistance with design, synthesis, screening, and decoding of OBOC peptide libraries. Utilization of this Shared Resource was supported by the UC Davis Comprehensive Cancer Center Support Grant (NCI P30CA093373). This work was supported by a grant from the Research Grants Council, HKSAR, China (CUHK 463911) and a grant from the Food Allergy and Anaphylaxis Network.
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Leung, N., Wai, C., Ho, M. et al. Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one-compound peptide libraries. Cell Mol Immunol 14, 308–318 (2017). https://doi.org/10.1038/cmi.2015.83
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DOI: https://doi.org/10.1038/cmi.2015.83
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