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PFA-fixed Hsp60sp-loaded dendritic cells as a vaccine for the control of mouse experimental allergic encephalomyelitis

Cellular & Molecular Immunology volume 11, pages 169–174 (2014)Cite this article

Abstract

We have shown that Hsp60sp-loaded immature dendritic cells (DC/sp) can protect mice from the induction of experimental allergic encephalomyelitis (EAE) by inducing Qa-1-restricted CD8+ T regulatory (Treg) cells. The binding half-life between Qa-1 and Hsp60sp is particularly short and leads to an unstable Qa-1/peptide complex that significantly decreases the efficacy of this vaccination. To prevent Qa-1/Hsp60sp complex dissociation, we utilized paraformaldehyde (PFA) fixation to stabilize the formation of the Qa-1/Hsp60sp complex and maximize the function of DC/sp as a vaccine to control autoimmune diseases. Compared with the non-fixed DC/sp, the fixed DC/sp (FDC/sp) showed an enhanced ability to activate Qa-1-restricted Hsp60sp-specific CD8+T cells in vitro and prevented EAE in vivo. Importantly, the FDC/sp maintained immune activity following cryopreservation for 1 week or after storage for 72 h at 4 °C. These results indicate that PFA fixation can sustain or increase the efficacy of DC/sp by improving the stability of the Qa-1/Hsp60sp complex on the surface of the DC/sp. In addition, PFA fixation creates a time window for DC/sp storage, transport and application. Our data suggest a potential clinical use of FDC/sp as a vaccine for the prevention and treatment of autoimmune disease.

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Acknowledgements

This work has been supported by the National Natural Science Foundation of China (NSF-30830093) and National Key Program (973) for Basic Research of China (2009CB522409) to HJ.

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  1. Feng Liu and Hui Zheng: These authors contributed equally to this work.

Authors and Affiliations

  1. Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China

    Feng Liu, Hui Zheng, Yuanyuan Qi, Xue Wang, Jianjun Yang, Miaomiao Han, Han Zhang & Hong Jiang

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  1. Feng Liu
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  2. Hui Zheng
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Correspondence to Hong Jiang.

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Supplementary Information accompanies the paper on Cellular &Molecular Immunology's website.

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Liu, F., Zheng, H., Qi, Y. et al. PFA-fixed Hsp60sp-loaded dendritic cells as a vaccine for the control of mouse experimental allergic encephalomyelitis. Cell Mol Immunol 11, 169–174 (2014). https://doi.org/10.1038/cmi.2013.58

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