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Microparticles released by Listeria monocytogenes-infected macrophages are required for dendritic cell-elicited protective immunity

Cellular & Molecular Immunology volume 9, pages 489–496 (2012)Cite this article

Abstract

Interplay between macrophages and dendritic cells in the processing and presentation of bacterial antigens for T-cell immune responses remains poorly understood. Using a Listeria monocytogenes (Lm) infection model, we demonstrate that dendritic cells (DCs) require the support of macrophages to elicit protective immunity against Lm infection. DCs themselves were inefficient at taking up Lm but capable of taking up microparticles (MPs) released by Lm-infected macrophages. These MPs transferred Lm antigens to DCs, allowing DCs to present Lm antigen to effector T cells. MP-mediated Lm antigen transfer required MHC class I participation, since MHC class I deficiency in macrophages resulted in a significant reduction of T-cell activation. Moreover, the vaccination of mice with MPs from Lm-infected macrophages produced strong protective immunity against Lm infection. We here identify an intrinsic antigen transfer program between macrophages and DCs during Lm infection, and emphasize that macrophages also play an essential role in DC-elicited Lm-specific T-cell responses.

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Acknowledgements

This work was supported by the National Basic Research Program of China (2012CB932500), Funds for International Cooperation and Exchange of the National Natural Science Foundation of China (30911120482), the Program for New Century Excellent Talents in University (NCET-08-0219) and the Fundamental Research Funds for the Central Universities (HUST-2010JC024, HUST-2011TS027).

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Author notes

  1. Yi Zhang and Ruihua Zhang: These two authors contributed equally to this work.

  2. Dr B Huang, Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. E-mail: tjhuangbo@hotmail.com

  3. Dr HB Xiong, Immunology Institute, Box 1630, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, USA. E-mail: Huabao.Xiong@mssm.edu

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Yi Zhang, Huafeng Zhang, Jing Liu, Zhuoshun Yang, Pingwei Xu, Wenqian Cai & Bo Huang

  2. Immunology Institute, Mount Sinai School of Medicine, New York, NY, USA

    Ruihua Zhang, Geming Lu, Miao Cui & Huabao Xiong

  3. Laboratory of Liposome Research, Institute of Molecular Cancer Research, University of Zurich, Zurich, Switzerland

    Reto A Schwendener

  4. Department of Respiratory Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Huang-Zhong Shi

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Zhang, Y., Zhang, R., Zhang, H. et al. Microparticles released by Listeria monocytogenes-infected macrophages are required for dendritic cell-elicited protective immunity. Cell Mol Immunol 9, 489–496 (2012). https://doi.org/10.1038/cmi.2012.33

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