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Eliciting protective immune responses against murine myeloma challenge in lymphopenia mice through adoptive transfer of tumor antigen-specific lymphocytes and immunization of tumor vaccine secreting mIL-21

Cancer Gene Therapy volume 17, pages 675–683 (2010)Cite this article

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Abstract

Previous studies have indicated that the cytokine interleukin (IL)-21 may induce both innate and adaptive immune responses against tumors. The goal of this study was to evaluate a new adoptive immunotherapy strategy that combined lymphocytes from mice immunized with a murine myeloma vaccine secreting murine IL-21 (mIL-21-Sp2/0) in lymphopenic mice induced by cyclophosphamide. The data indicate that effective antitumor immunity was induced in mice receiving syngeneic murine lymphocytes from the mice immunized with the mIL-21-Sp2/0. More importantly, the efficacy against the Sp2/0 cell challenge was enhanced after the lymphocytes were activated and proliferated ex vivo before administration into the lymphopenic mice. We conclude that the adoptive transfer of tumor antigen-specific lymphocytes into mice immunized with mIL-21-Sp2/0 induced protective immune responses against myeloma challenge.

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Acknowledgements

We thank Dr Aifeng Zhang for the assistance in histological section and analysis, and Dr Rod Donlan (Centers for Disease Control and Prevention in Atlanta, GA, USA) for kindly reviewing the article. This work was supported in part by the Program for the Top Researchers in Six Fields of Jiangsu Province, China (no. D14) and in part by the 973 Program of China (no. 2006CB933206).

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Author notes

  1. J Dou, Y Wu and J Wang: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Pathogenic Biology and Immunology, School of Basic Medical Science, Southeast University, Nanjing, China

    J Dou, Y Wu, F Zhao, L Chu, C Liu, P Wen, W Hu, K Hu & X F He

  2. Department of Gynecology and Obstetrics, Zhongda Hospital, Southeast University, Nanjing, China

    J Wang

  3. Jiangsu Laboratory for Biomaterials and Devices, Paediatric Research Institute, Nanjing Children's Hospital Nanjing, Nanjing, China

    L Chu

  4. Jiangsu Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China

    N Gu

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  1. J Dou
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Correspondence to J Dou or N Gu.

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Dou, J., Wu, Y., Wang, J. et al. Eliciting protective immune responses against murine myeloma challenge in lymphopenia mice through adoptive transfer of tumor antigen-specific lymphocytes and immunization of tumor vaccine secreting mIL-21. Cancer Gene Ther 17, 675–683 (2010). https://doi.org/10.1038/cgt.2010.23

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