Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Effect of sonoporation on cationic liposome-mediated IFNβ gene therapy for metastatic hepatic tumors of murine colon cancer

Cancer Gene Therapy volume 16, pages 638–643 (2009)Cite this article

A Corrigendum to this article was published on 16 September 2009

Abstract

To examine the efficacy of ultrasound-mediated gene therapy in the treatment of hepatic cancer, we performed cationic liposome-mediated interferon-β (IFNβ) gene therapy combined with sonoporation, using metastatic hepatic tumors of murine colon cancer. The combination of liposome/DNA treatment and ultrasound gave higher transgene IFNβ expression in Colon26 colon adenocarcinoma cells than that with microbubble/DNA in combination with ultrasound and that with liposome/DNA alone. Cationic liposome-mediated IFNβ gene therapy combined with ultrasound showed an antitumor effect in vitro and combination use of the anticancer drug cis-diamminedichloroplatinum (CDDP) showed a synergistic effect with the IFNβ gene therapy. The survival of mice with metastatic hepatic tumor of Colon26 cells was significantly prolonged by cationic liposome-mediated IFNβ gene therapy alone, and sonoporation had a synergistic effect on prolongation of survival and inhibition of tumor growth rate in these mice. Combination use of CDDP with IFNβ gene therapy also showed a synergistic effect on prolongation of survival and inhibition of tumor growth rate. In conclusion, ultrasound-mediated gene therapy for the treatment of hepatic cancers may be effective in a clinical setting.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3

Similar content being viewed by others

References

  1. Kim HJ, Greenleaf JF, Kinnick RR, Bronk JT, Bolander ME . Ultrasound-mediated transfection of mammalian cells. Hum Gene Ther 1996; 7: 1339–1346.

    Article  CAS  Google Scholar 

  2. Tachibana K, Uchida T, Ogawa K, Yamashita N, Tamura K . Induction of cell-membrane porosity by ultrasound. Lancet 1999; 353: 1409.

    Article  CAS  Google Scholar 

  3. Ward M, Wu J . Ultrasound-induced cell lysis and sonoporation enhanced by contrast agents. J Acoust Soc Am 1999; 105: 2951–2957.

    Article  CAS  Google Scholar 

  4. Taniyama Y, Tachibana K, Hiraoka K, Namba T, Yamasaki K, Hashiya N et al. Local delivery of plasmid DNA into rat carotid artery using ultrasound. Circulation 2002; 105: 1233–1239.

    Article  CAS  Google Scholar 

  5. Unger EC, McCreery TP, Sweitzer RH . Ultrasound enhances gene expression of liposomal transfection. Invest Radiol 1997; 32: 723–727.

    Article  CAS  Google Scholar 

  6. Vile RG, Russell SJ, Lemoine NR . Cancer gene therapy: hard lessons and new courses. Gene Therapy 2000; 7: 2–8.

    Article  CAS  Google Scholar 

  7. Kirn D . Clinical research results with dl1520 (Onyx-015), a replication-selective adenovirus for the treatment of cancer: what have we learned? Gene Therapy 2001; 8: 89–98.

    Article  CAS  Google Scholar 

  8. Ra SE, Chirmule N, Lee FS, Wivel NA, Bagg A, Gao GP et al. Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transfer. Mol Genet Metab 2003; 80: 148–158.

    Article  Google Scholar 

  9. Mizuno M, Yoshida J, Sugita K, Inoue I, Seo H, Hayashi Y et al. Growth inhibition of glioma cells transfected with the human β-interferon gene by liposome coupled with a monoclonal antibody. Cancer Res 1990; 50: 7826–7829.

    CAS  PubMed  Google Scholar 

  10. Nakanishi H, Mizutani Y, Kawauchi A, Ukimura O, Shiraishi T, Hatano M et al. Significant antitumoral activity of cationic multilamellar liposomes containing human IFN-beta gene against human renal cell carcinoma. Clin Cancer Res 2003; 9: 1129–1135.

    CAS  PubMed  Google Scholar 

  11. Haga N, Yagi K . An improvement method for entrapment of plasmids in liposomes. J Clin Biochem Nutr 1989; 7: 175–183.

    Article  CAS  Google Scholar 

  12. Szoka F, Papahadjopoulos D . Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc Natl Acad Sci USA 1978; 75: 4194–4198.

    Article  CAS  Google Scholar 

  13. Yoshida J, Mizuno M, Fujii M, Kajita Y, Nakahara N, Hatano M et al. Humana gene therapy for malignant gliomas ( glioblastoma multiforme and anaplastic astrocytoma) by in vivo transduction with human interferon beta gene using cationic liposomes. Hum Gene Ther 2004; 15: 77–86.

    Article  CAS  Google Scholar 

  14. Koch S, Pohl P, Cobet U, Rainov NG . Ultrasound enhancement of liposome-mediated cell transfection is caused by cavitation effects. Ultrasound Med Biol 2000; 26: 897–903.

    Article  CAS  Google Scholar 

  15. Shohet RV, Chen S, Zhou YT, Wang Z, Meidell RS, Unger RH et al. Echocardiographic destruction of albumin microbubbles directs gene delivery to the myocardium. Circulation 2000; 101: 2554–2556.

    Article  CAS  Google Scholar 

  16. Sakakima Y, Hayashi S, Yagi Y, Hayakawa A, Tachibana K, Nakao A . Gene therapy for hepatocellular carcinoma (HCC) using sonoporation enhanced by contrast agent. Cancer Gene Ther 2005; 12: 884–889.

    Article  CAS  Google Scholar 

  17. Natsume A, Mizuno M, Ryuke Y, Yoshida J . Antitumor effect and cellular immunity activation by murine interferon-beta gene transfer against intracerebral glioma in mouse. Gene Therapy 1999; 6: 1626–1633.

    Article  CAS  Google Scholar 

  18. Natsume A, Tsujjimura K, Mizuno M, Takahashi T, Yoshida J . IFN-beta gene therapy induces systemic antitumor immunity against malignant glioma. J Neurooncol 2000; 47: 117–124.

    Article  CAS  Google Scholar 

  19. Kageshita T, Mizuno M, Ono T, Matsumoto K, Saida T, Yoshida J . Growth inhibition of human malignant melanoma transfected with the human interferon-beta gene by means of cationic liposomes. Melanoma Res 2001; 11: 337–342.

    Article  CAS  Google Scholar 

  20. Manome Y, Nakamura M, Ohno T, Furuhata H . Ultrasound facilitates transduction of naked plasmid DNA into colon carcinoma cells in vitro and in vivo. Hum Gene Ther 2000; 11: 1521–1528.

    Article  CAS  Google Scholar 

  21. Michel MS, Erben P, Trojan L, Schaaf A, Kiknavelidze K, Knoll T et al. Acoustic energy: a new transfection method for cancer of the prostate, cancer of the bladder and benign kidney cells. Anticancer Res 2004; 24: 2303–2308.

    CAS  PubMed  Google Scholar 

  22. Fujiwara T, Grimm EA, Mukhopadhyay T, Zhang WW, Owen-Schaub LB, Roth JA . Induction of chemosensitivity in human lung cancer cells in vivo by adenovirus-mediated transfer of the wild-type p53 gene. Cancer Res 1994; 54: 2287–2291.

    CAS  PubMed  Google Scholar 

  23. Takaoka A, Hayakawa S, Yanai H, Stoiber D, Negishi H, Kikuchi H et al. Integration of interferon-α/β signaling to p53 responses in tumor suppression and antiviral defense. Nature 2003; 424: 516–523.

    Article  CAS  Google Scholar 

  24. Shimizu M, Akiyama S, Ito K, Kasai Y, Takagi H, Kito M et al. Anti-proliferative effects on human colon cancer cells of combined treatment with cationic liposome-entrapped human interferon-β or –γ gene and anti-cancer drug. J Clin Biochem Nutr 1997; 23: 77–83.

    Article  CAS  Google Scholar 

  25. Anwer K, Kao G, Proctor B, Anscombe I, Florack V, Earls R et al. Ultrasound enhancement of cationic lipid-mediated gene transfer to primary tumors following systemic administration. Gene Therapy 2000; 7: 1833–1839.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University, School of Medicine, 65 Tsurumai, Showa-ku, Nagoya, Japan

    S Hayashi

  2. Department of Neurosurgery, Nagoya University, School of Medicine, 65 Tsurumai, Showa-ku, Nagoya, Japan

    M Mizuno & J Yoshida

  3. Department of Surgery, Nagoya University, School of Medicine, 65 Tsurumai, Showa-ku, Nagoya, Japan

    A Nakao

Authors
  1. S Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M Mizuno
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J Yoshida
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A Nakao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S Hayashi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hayashi, S., Mizuno, M., Yoshida, J. et al. Effect of sonoporation on cationic liposome-mediated IFNβ gene therapy for metastatic hepatic tumors of murine colon cancer. Cancer Gene Ther 16, 638–643 (2009). https://doi.org/10.1038/cgt.2008.1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/cgt.2008.1

Keywords

This article is cited by

Search

Advanced search

Quick links