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Feasibility of marrow harvesting from pediatric sibling donors without hematopoietic growth factors and allotransfusion

Bone Marrow Transplantation volume 49, pages 921–926 (2014)Cite this article

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Abstract

We retrospectively studied 108 marrow harvests from 105 pediatric sibling donors. The median age of donors was 8 years (range: 1–15) and the median body weight was 27 kg (range: 10–100). The volumes of aspirated marrow were 5.0–23.8 mL/kg donor body weight, and harvested bone marrow volume exceeded 15 mL/kg in 42% of the donors. A total of 100 autologous blood donations were performed, and eight donors had red cells salvaged from their harvests reinfused. The median Hb levels before and after harvests were 12.3 g/dL (range: 10.0–14.7) and 11.0 g/dL (range: 8.9–13.8), respectively. None of the donors received allogeneic blood transfusions or hematopoietic growth factors such as EPO and G-CSF before or after collection. Transplanted dose was 1.4–10.8 × 108 cells/kg recipient body weight without differences due to donor age. Higher concentrations of nucleated and CD34+ cells were obtained from younger donors. All donors tolerated the procedures well, with no serious complications. Thus, children may safely donate marrow for allogeneic transplantation, and the yields of nucleated cells for engraftment are substantial.

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Acknowledgements

We would like to thank the individual donors, patients and families in the study who made this work possible, and the members in the departments of blood transfusion service and cell transplantation and regenerative medicine of Tokai university hospital for their expert technical assistance.

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Authors and Affiliations

  1. Department of Cell Transplantation and Regenerative Medicine, Tokai University Hospital, Isehara, Japan

    M Yabe, S Kato & H Yabe

  2. Department of Pediatrics, Tokai University Hospital, Isehara, Japan

    T Morimoto, T Shimizu, T Koike, H Takakura, K Ohtsubo & A Fukumura

Authors
  1. M Yabe
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  2. T Morimoto
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  3. T Shimizu
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  4. T Koike
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  5. H Takakura
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  7. A Fukumura
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  8. S Kato
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Correspondence to H Yabe.

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Yabe, M., Morimoto, T., Shimizu, T. et al. Feasibility of marrow harvesting from pediatric sibling donors without hematopoietic growth factors and allotransfusion. Bone Marrow Transplant 49, 921–926 (2014). https://doi.org/10.1038/bmt.2014.73

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  • DOI: https://doi.org/10.1038/bmt.2014.73

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