Abstract
Mobilized peripheral blood (mPB) is a prevalent source of hematopoietic progenitors for transplantation; however, allogeneic and haploidentical transplants are often accompanied by severe GVHD. Following the observation that murine GVHD is ameliorated by pretransplant donor cell exposure to Fas-ligand (FasL) without host-specific sensitization, we assessed the susceptibility of mPB cells to spontaneous and receptor-induced apoptosis as a possible approach to GVHD prophylaxis. Short incubation for 4 h resulted in spontaneous apoptosis of 50% of the T and B lymphocytes and 60% myeloid cells. Although expression of Fas and TNF-R1 was proportionate to fractional apoptosis, cell death was dominated by spontaneous apoptosis. Functional assays revealed that the death receptors modulated mPB graft composition as compared with incubation in medium, without detectable quantitative variations. Removal of dead cells increased the frequency of mPB myeloid progenitors (P<0.001 vs medium), and recipients of mPB exposed to death ligands displayed reduced GVHD (P<0.01 vs medium) and improved survival following lipopolysacharide stimulation. mPB grafts exposed to the apoptotic challenge retained SCID reconstituting potential and graft versus tumor activity. These data emphasize that short-term exposure of mPB grafts to an apoptotic challenge is effective in reduction of GVHD effector activity.
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References
Shlomchik WD . Graft-versus-host disease. Nat Rev Immunol 2007; 7: 340–352.
Ferrara JL, Levine JE, Reddy P, Holler E . Graft-versus-host disease. Lancet 2009; 373: 1550–1561.
Ullrich SE . Photoinactivation of T-cell function with psoralen and UVA radiation suppresses the induction of experimental murine graft-versus-host disease across major histocompatibility barriers. J Invest Dermatol 1991; 96: 303–308.
Grass JA, Wafa T, Reames A, Wages D, Corash L, Ferrara JL et al. Prevention of transfusion-associated graft-versus-host disease by photochemical treatment. Blood 1999; 93: 3140–3147.
Waller EK, Boyer M . New strategies in allogeneic stem cell transplantation: immunotherapy using irradiated allogeneic T cells. Bone Marrow Transplant 2000; 25 (Suppl 2): S20–S24.
Giver CR, Montes RO, Mittelstaedt S, Li JM, Jaye DL, Lonial S et al. Ex vivo fludarabine exposure inhibits graft-versus-host activity of allogeneic T cells while preserving graft-versus-leukemia effects. Biol Blood Marrow Transplant 2003; 9: 616–632.
Godfrey W1R, Krampf MR, Taylor PA, Blazar BR . Ex vivo depletion of alloreactive cells based on CFSE dye dilution, activation antigen selection, and dendritic cell stimulation. Blood 2004; 103: 1158–1165.
Matsue H, Matsue K, Kusuhara M, Kumamoto T, Okumura K, Yagita H et al. Immunosuppressive properties of CD95L-transduced ‘killer’ hybrids created by fusing donor- and recipient-derived dendritic cells. Blood 2001; 98: 3465–3472.
Hartwig UF, Robbers M, Wickenhauser C, Huber C . Murine acute graft-versus-host disease can be prevented by depletion of alloreactive T lymphocytes using activation-induced cell death. Blood 2002; 99: 3041–3049.
Georgantas RW, Bohana-Kashtan O, Civin CI . Ex vivo soluble fas ligand treatment of donor cells to selectively reduce murine acute graft versus host disease. Transplantation 2006; 82: 471–478.
Bohana-Kashtan O, Morisot S, Hildreth R, Brayton C, Levitsky HI, Civin CI . Selective reduction of graft-versus-host disease-mediating human T cells by ex vivo treatment with soluble Fas ligand. J Immunol 2009; 183: 696–705.
Morecki S, Gelfand Y, Yacovlev E, Eizik O, Shabat Y, Sagiv I et al. Selective elimination of alloreactivity in vitro and in vivo while sparing other T-cell-mediated immune responses. Bone Marrow Transplant 2012; 47: 838–845.
Yarkoni S, Prigozhina TB, Slavin S, Askenasy N . IL-2-targeted therapy ameliorates the severity of graft-versus-host disease: ex vivo selective depletion of host-reactive T cells and in vivo therapy. Biol Blood Marrow Transplant 2012; 18: 523–535.
Askenasy N, Mizrahi K, Ash S, Askenasy EM, Yaniv I, Stein J . Depletion of naïve lymphocytes with Fas-ligand ex vivo prevents GvHD without impairing T cell support of engraftment or GVT activity. Biol Blood Marrow Transplant 2013; 19: 185–195.
Pearl-Yafe M, Yolcu ES, Stein J, Kaplan O, Shirwan H, Yaniv I et al. Expression of Fas and Fas-ligand in donor hematopoietic stem and progenitor cells is dissociated from the sensitivity to apoptosis. Exp Hematol 2007; 35: 1601–1612.
Pearl-Yafe M, Stein J, Yolcu ES, Farkas DL, Shirwan H, Yaniv I et al. Fas transduces dual apoptotic and trophic signals in hematopoietic progenitors. Stem Cells 2007; 25: 3194–3203.
Pearl-Yafe M, Mizrahi K, Stein J, Yolcu ES, Kaplan O, Shirwan H et al. Tumor necrosis factor receptors support murine hematopoietic progenitor function in the early stages of engraftment. Stem Cells 2010; 28: 1270–1280.
Askenasy N, Mizrahi K, Ash S, Askenasy EM, Yaniv I, Stein J . Regulatory functions of TRAIL in hematopoietic progenitors: human umbilical cord blood and murine bone marrow transplantation. Leukemia 2010; 24: 1325–1334.
Mizrahi K, Stein J, Yaniv I, Kaplan O, Askenasy N . TNF-α has tropic rather than apoptotic activity in human hematopoietic progenitors: involvement of TNF receptor-1 and caspase-8. Stem Cells 2013; 31: 156–166.
Kaminitz A, Yolcu ES, Askenasy EM, Stein J, Yaniv I, Shirwan H et al. Effector and naturally occurring regulatory T cells display no abnormalities in activation induced cell death in NOD mice. PLoS One 2011; 6: e21630.
Ash S, Gigi V, Askenasy N, Fabian I, Stein J, Yaniv I . Graft versus neuroblastoma reaction is efficiently elicited by allogeneic bone marrow transpl1antation through cytolytic activity in the absence of GVHD. Cancer Immunol Immunother 2009; 58: 2073–2084.
Reuveni D, Halperin D, Fabian I, Tsarfaty G, Askenasy N, Shalit I . Moxifloxacin increases anti-tumor and anti-angiogenic activity of irinotecan in human xenograft tumors. Biochem Pharmacol 2010; 79: 1100–1107.
Kaminitz A, Askenasy EM, Yaniv I, Stein J, Askenasy N . Apoptosis of purified CD4+ T cell subsets is dominated by cytokine deprivation and absence of other cells in new onset diabetic NOD mice. PLoS One 2010; 5: e15684.
Peters R, Leyvraz S, Perey L . Apoptotic regulation in primitive hematopoietic precursors. Blood 1998; 92: 2041–2052.
Pyatt DW, Stillman WS, Yang Y, Gross S, Zheng JH, Irons RD . An essential role for NF-{kappa}B in human CD34+ bone marrow cell survival. Blood 1999; 93: 3302–3308.
Maurillo L, Del Poeta G, Venditti A, Buccisano F, Battaglia A, Santinelli S et al. Quantitative analysis of Fas and bcl-2 expression in hematopoietic precursors. Haematologica 2001; 86: 237–243.
Fukuda S, Pelus LM . Regulation of the inhibitor-of-apoptosis family member survivin in normal cord blood and bone marrow CD34 cells by hematopoietic growth factors: implication of survivin expression in normal hematopoiesis. Blood 2001; 98: 2091–2100.
Kim H, Whartenby KA, Georgantas RW, Wingard J, Civin CI . Human CD34+ hematopoietic stem/progenitor cells express high levels of FLIP and are resistant to Fas-mediated apoptosis. Stem Cells 2002; 20: 174–182.
Gothot A, van der Loo JCM, Clapp DW, Srour EF . Cell cycle–related changes in repopulating capacity of human mobilized peripheral blood CD34 cells in non-obese diabetic/severe combined immunedeficient mice. Blood 1998; 92: 2641–2649.
Danet GH, Lee HW, Luongo JL, Simon MC, Bonnet DA . Dissociation between stem cell phenotype and NOD/SCID repopulating activity in human peripheral blood CD34(+) cells after ex vivo expansion. Exp Hematol 2001; 29: 1465–1473.
Young JC, Lin K, Travis M, Hansteen G, Abitorabi A, Sirenko O et al. Investigation into an engraftment defect induced by culturing primitive hematopoietic cells with cytokines. Cytotherapy 2001; 3: 307–320.
Iwasaki T, Hamano T, Saheki K, Kuroiwa T, Kataoka Y, Takemoto Y et al. Effect of graft-versus-host disease (GVHD) on host hematopoietic progenitor cells is mediated by Fas-Fas ligand interactions but this does not explain the effect of GVHD on donor cells. Cell Immunol 1999; 197: 30–38.
Jiang Z, Adams GB, Hanash AM, Scadden DT, Levy RB . The contribution of cytotoxic and noncytotoxic function by donor T-cells that support engraftment after allogeneic bone marrow transplantation. Biol Blood Marrow Transplant 2002; 8: 588–596.
Marks L, Altman NH, Podack ER, Levy RB . Donor T cells lacking Fas ligand and perforin retain the capacity to induce severe GvHD in minor histocompatibility antigen mismatched bone-marrow transplantation recipients. Transplantation 2004; 77: 804–812.
Mayumi H, Good RA . Long-lasting skin allograft tolerance in adult mice induced across fully allogeneic (multimajor H-2 plus multiminor histocompatibility) antigen barriers by a tolerance-inducing method using cyclophosphamide. J Exp Med 1989; 169: 213–238.
Colson YL, Wren SM, Schuchert MJ, Patrene KD, Johnson PC, Boggs SS et al. A nonlethal conditioning approach to achieve durable multilineage mixed chimerism and tolerance across major, minor, and hematopoietic histocompatibility barriers. J Immunol 1995; 155: 4179–4188.
Truitt RL, Johnson BD, Hanke C, Talib S, Hearst JE . Photochemical treatment with S-59 psoralen and ultraviolet A light to control the fate of naive or primed T lymphocytes in vivo after allogeneic bone marrow transplantation. J Immunol 1999; 163: 5145–5156.
Hannani D, Merlin E, Gabert F, Laurin D, Deméocq F, Chaperot L et al. Photochemotherapy induces a faster apoptosis of alloreactive activated T cells than of nonalloreactive resting T cells in graft versus host disease. Transplantation 2010; 90: 1232–1238.
Morecki S, Yacovlev E, Gelfand Y, Eizik O, Slavin S . Pretransplant treatment of donors with immunomodulators to control graft-versus-host disease (GVHD) in transplant recipients. Exp Hematol 2007; 35: 748–756.
Morecki S, Yacovlev E, Gelfand Y, Shabat Y, Slavin S . Induction of graft-versus-leukemia (GVL) effect without graft-versus-host disease (GVHD) by pretransplant donor treatment with immunomodulators. Biol Blood Marrow Transplant 2009; 15: 406–415.
Blazar BR, Carroll SF, Vallera DA . Prevention of murine graft-versus-host disease and bone marrow alloengraftment across the major histocompatibility barrier after donor graft preincubation with anti-LFA1 immunotoxin. Blood 1991; 78: 3093–3102.
Drobyski WR, Majewski D, Ozker K, Hanson G . Ex vivo anti-CD3 antibody-activated donor T cells have a reduced ability to cause lethal murine graft-versus-host disease but retain their ability to facilitate alloengraftment. J Immunol 1998; 161: 2610–2619.
Koh MB, Prentice HG, Corbo M, Morgan M, Cotter FE, Lowdell MW . Alloantigen-specific T-cell depletion in a major histocompatibility complex fully mismatched murine model provides effective graft-versus-host disease prophylaxis in the presence of lymphoid engraftment. Br J Haematol 2002; 118: 108–116.
Chen HR, Ji SQ, Wang HX, Yan HM, Zhu L, Liu J et al. Humanized anti-CD25 monoclonal antibody for prophylaxis of graft-vs-host disease (GVHD) in haploidentical bone marrow transplantation without ex vivo T-cell depletion. Exp Hematol 2003; 31: 1019–1025.
Davies JK, Koh MB, Lowdell MW . Antiviral immunity and T-regulatory cell function are retained after selective alloreactive T-cell depletion in both the HLA-identical and HLA-mismatched settings. Biol Blood Marrow Transplant 2004; 10: 259–268.
Ji SQ, Chen HR, Yan HM, Wang HX, Liu J, Zhu PY et al. Anti-CD25 monoclonal antibody (basiliximab) for prevention of graft-versus-host disease after haploidentical bone marrow transplantation for hematological malignancies. Bone Marrow Transplant 2005; 36: 349–354.
Bastien JP, Roy J, Roy DC . Selective T-cell depletion for haplotype-mismatched allogeneic stem cell transplantation. Semin Oncol 2012; 39: 674–682.
Askenasy N, Yolcu ES, Yaniv I, Shirwan H . Induction of tolerance using Fas ligand: a double-edged immunomodulator. Blood 2005; 105: 1396–1404.
Askenasy N . Hematopoietic transplants for disease suppression and cure in type 1 diabetes. Curr Stem Cell Res Ther 2013; 8: 333–339.
Ng YY, van Kessel B, Lokhorst HM, Baert MR, van den Burg CM, Bloem AC et al. Gene-expression profiling of CD34+ cells from various hematopoietic stem-cell sources reveal1s functional differences instem-cell activity. J Leukoc Biol 2004; 75: 314–323.
Huang S, Law P, Young D, Ho AD . Candidate hematopoietic stem cells from fetal tissues, umbilical cord blood vs adult bone marrow and mobilized peripheral blood. Exp Hematol 1998; 26: 1162–1171.
Tanavde VM, Malehorn MT, Lumkul R, Gao Z, Wingard J, Garrett ES et al. Human stem-progenitor cells from neonatal cord blood have greater hematopoietic expansion capacity than those from mobilized adult blood. Exp Hematol 2002; 30: 816–823.
Srour EF, Bregni M, Traycoff CM, Agüero B, Kosak ST, Hoffman R et al. Long-term hematopoietic culture-initiating cells are more abundant in mobilized peripheral blood grafts than in bone marrow but have a more limited ex vivo expansion potential. Blood Cells Mol Dis 1996; 22: 68–81.
Lemoli RM, Tafuri A, Fortuna A, Petrucci MT, Ricciardi MR, Catani L et al. Cycling status of CD34+ cells mobilized into peripheral blood of healthy donors by recombinant human granulocyte colony-stimulating factor. Blood 1997; 89: 1189–1196.
Traycoff CM, Orazi A, Ladd AC, Rice S, McMahel J, Srour EF . Proliferation-induced decline of primitive hematopoietic progenitor cell activity is coupled with an increase in apoptosis of ex vivo expanded CD34+ cells. Exp Hematol 1998; 26: 53–62.
Rutella S, Pierelli L, Sica S, Rumi C, Leone G . Transplantation of autologous peripheral blood progenitor cells: impact of CD34-cell selection on immunological reconstitution. Leuk Lymphoma 2001; 42: 1207–1220.
Chen YB, Cutler CS . Biomarkers for acute GVHD: can we predict the unpredictable? Bone Marrow Transplant 2013; 48: 755–760.
Paczesny S . Discovery and validation of graft-versus-host disease biomarkers. Blood 2013; 121: 585–594.
Pidala J, Sarwal M, Roedder S, Lee SJ . Biologic markers of chronic GVHD. Bone Marrow Transplant 2014; 49: 324–331.
Yolcu ES, Kaminitz A, Mizrahi K, Ash S, Yaniv I, Stein J et al. Immunomodulation with donor regulatory T cells armed with Fas-ligand alleviates graft-versus-host disease. Exp Hematol 2013; 41: 903–911.
Acknowledgements
This work was funded by grants from the Frankel Trust for Experimental Bone Marrow Transplantation. We thank Mrs Ela Zuzovsky and Mrs Ana Zemliansky for their outstanding technical support.
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Mizrahi, K., Yaniv, I., Ash, S. et al. Apoptotic signaling through Fas and TNF receptors ameliorates GVHD in mobilized peripheral blood grafts. Bone Marrow Transplant 49, 640–648 (2014). https://doi.org/10.1038/bmt.2014.12
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DOI: https://doi.org/10.1038/bmt.2014.12
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